Dissected larval pancreata were mounted in fluorescent mounting medium (Dako) and imaged with a Nikon A1-si Laser Scanning Confocal Head mounted on a TiE inverted microscope through a Plan Apo 20× VC (N.A. 0.75) (Nikon). Acquisition and analysis was performed using NIS-Elements software (Ver 3.22, build 710). Cell number was quantified by counterstaining nuclei with DAPI (0.2 μg/ml) and counted by using NIS-Elements software (Nikon).
A1 si laser scanning confocal head
Manufactured by Nikon
The A1-si Laser Scanning Confocal Head is a core component of Nikon's advanced confocal microscopy system. It is designed to provide high-resolution, high-sensitivity imaging capabilities for various scientific and research applications. The A1-si utilizes laser excitation and a confocal pinhole arrangement to enable precise optical sectioning and improved signal-to-noise ratio, allowing for the capture of detailed, three-dimensional images of samples.
Automatically generated - may contain errors
2 protocols using a1 si laser scanning confocal head
1
Quantifying Pancreatic Cell Abundance
2
Quantitative imaging of larval pancreata
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