Bone marrow-derived human mesenchymal stem cells (MSCs) were obtained from Lonza. MSCs were cultured directly on TCPS in Minimal Essential Media-α (CellGro) with 5% Penicillin/Streptomycin (Gibco) and 10% v/v fetal bovine serum (Gibco). MSCs were used between passage 7 and passage 10. Detachment for passage and when needed for experiments was accomplished via treatment with trypsin (Hyclone) for 5 min and subsequent washing of the cell culture surface. MSCs were recovered via centrifugation at 0.2 kRCF for 5 min, counted on a hemocytometer, and seeded directly into the experiment or a new TCPS culture flask.
Minimal essential media α
Manufactured by Corning
Minimal Essential Media-α (MEM-α) is a cell culture medium used to support the growth and maintenance of a variety of cell types in vitro. It provides a balanced salt solution, amino acids, vitamins, and other nutrients essential for cell proliferation and viability.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using minimal essential media α
1
Expansion of Human Mesenchymal Stem Cells
2
Culturing Human Dermal Fibroblasts
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!