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2 protocols using p toluene sulphonic acidmonohydrate

1

Synthesis and Characterization of Bioactive Compounds

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All
the reagents studied
in the present investigation (bromoacetic acid, n-octanol, n-hexane, ethyl acetate, chloroform, N-methyl imidazole, methanol, NaCl, MgCl2, CaCl2, sodium alginate, p-toluene sulphonic acid
monohydrate, sodium salicylate, CV, MO, AR, RB, and curcumin) were
of analytical grade, purchased from Sigma-Aldrich, and used as received.
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2

Mutagenicity Assay Chemical Reagents

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Acetoin ≥95%, dimethyl carbonate 99%, p-toluenesulphonic acid monohydrate 98.5%, 4-methoxyacetophenone 99%, anhydrous anisole 99.7%, acetic anhydride ≥99%, anhydrous acetonitrile 99.8%, anhydrous cyclohexane 99.5%, anhydrous diethyl ether ≥99%, anhydrous propylene carbonate 99.7%, iron(iii) chloride 97%, anhydrous ethyl acetate 99.8%, anhydrous magnesium sulphate 99.5%, Nile red ≥98%, 4-nitroaniline ≥99%, 4-nitroanisole analytical standard, 4-nitrophenol spectrophotometric grade, 1,2-dimethylimidazole 98%, 1-bromodecane 98%, methanol 99.9%, dichloromethane 99.8%, acetone 99.9%, chloroform-d (CDCl3, 99.8% D), chloroform 99.9%, dimethyl sulfoxide 99.9% were purchased from Sigma-Aldrich. Ames MPF 98/100 kits, 2-nitrofluorene and 4-nitroquinoline-N-oxide were purchased from Xenometrix. TA98 and TA100 were stored at −70 °C. QUANTOFIX® Peroxide 100 was purchased from Macherey-Nagel. N,N-Diethyl-4-nitroaniline was purchased from VWR.
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