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Grass sd9k stimulator

Manufactured by Natus
Sourced in Canada

The Grass SD9K stimulator is a laboratory device used to generate electrical stimuli. It is designed to provide controlled and programmable electrical pulses for various applications in research and clinical settings. The core function of the SD9K is to deliver consistent and repeatable electrical stimulation to study physiological responses or to elicit specific reactions in experimental subjects.

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Lab products found in correlation

2 protocols using grass sd9k stimulator

1

Isolation of Hippocampal Neurons from CA1 Region

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Individual hippocampal neurons were isolated from the CA1 region using an enzyme-free mechanical dissociation procedure, as described previously (Akaike and Moorhouse, 2003 (link); Jun et al., 2011 ; Vorobjev, 1991 (link)). Briefly, slices including hippocampus were transferred into a 35 mm culture dish containing an external recording buffer with the following composition (in mM): 150 NaCl, 5 KCl, 2.5 CaCl2, 1 MgCl2, 10 HEPES, and 10 D-Glucose, pH adjusted to 7.4 with NaOH, osmolarity adjusted to 320 mOsm with sucrose. A fire-polished glass micropipette was placed on the surface of the CA1 region. The pipette tip was vibrated horizontally within the CA1 region at 30Hz over a distance of 100–200 μm for ~2 min using a piezoelectric manipulator (LSS-3000, Burleigh/Exfo, Quebec, Canada) triggered by a Grass SD9K stimulator (Grass Technologies, West Warwick, RI). After the slice was removed, the isolated neurons were allowed to settle on the bottom of the dish for 10–15 min. For purely electrophysiological experiments, cells were isolated/examined in standard 35 mm cell culture dishes, (BD Biosciences, San Jose, CA). For experiments involving imaging, cells were isolated/examined in 35 mm cell culture dishes containing a glass coverslip insert (WillCo Wells B.V., Amsterdam, Netherlands). Cover-slips were coated with poly-l-lysine prior to use.
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2

Isolation of Hippocampal Neurons from CA1 Region

Check if the same lab product or an alternative is used in the 5 most similar protocols
Individual hippocampal neurons were isolated from the CA1 region using an enzyme-free mechanical dissociation procedure, as described previously (Akaike and Moorhouse, 2003; Jun et al., 2011; Vorobjev, 1991) . Briefly, slices including hippocampus were transferred into a 35 mm culture dish containing an external recording buffer with the following composition (in mM): 150 NaCl, 5 KCl, 2.5 CaCl 2 , 1 MgCl 2 , 10 HEPES, and 10 D-Glucose, pH adjusted to 7.4 with NaOH, osmolarity adjusted to 320 mOsm with sucrose. A firepolished glass micropipette was placed on the surface of the CA1 region. The pipette tip was vibrated horizontally within the CA1 region at 30Hz over a distance of 100-200 μm for ~2 min using a piezoelectric manipulator (LSS-3000, Burleigh/Exfo, Quebec, Canada) triggered by a Grass SD9K stimulator (Grass Technologies, West Warwick, RI). After the slice was removed, the isolated neurons were allowed to settle on the bottom of the dish for 10-15 min. For purely electrophysiological experiments, cells were isolated/examined in standard 35 mm cell culture dishes, (BD Biosciences, San Jose, CA). For experiments involving imaging, cells were isolated/examined in 35 mm cell culture dishes containing a glass coverslip insert (WillCo Wells B.V., Amsterdam, Netherlands). Cover-slips were coated with poly-l-lysine prior to use.
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