Log In Sign up
The largest database of trusted experimental protocols

Cha-GAL80 is a genetic tool used in Drosophila research. It functions as a transcriptional repressor, inhibiting the activity of the GAL4 transcriptional activator in the presence of choline. This allows for spatiotemporal control of gene expression in Drosophila models.

Automatically generated - may contain errors

Lab products found in correlation

Gr43a gal4, by Bloomington Drosophila Stock Center (1 mentions) Gr64a gal4, by Bloomington Drosophila Stock Center (1 mentions) Gr5a gal4, by Bloomington Drosophila Stock Center (1 mentions) Tsh gal80, by Bloomington Drosophila Stock Center (1 mentions) Fru gal4, by Bloomington Drosophila Stock Center (1 mentions) Canton s strain, by Bloomington Drosophila Stock Center (1 mentions)

2 protocols using cha gal80

1

Drosophila Rearing and Genetic Manipulations

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flies (Drosophila melanogaster) were reared on a standard corn meal, yeast and glucose agar medium at 24.5 °C under a 12-h:12-h light: dark (LD) cycle as described before unless otherwise stated10 (link). Control (w1118) and UAS-TrpA1 fly stocks were described previously10 (link),13 (link). Gr5a-GAL4 (#57592), Gr43a-GAL4 (#57636) and Gr64a-GAL4 (#57661) were obtained from the Bloomington Stock Center; Cha-GAL80, Gad-GAL80 and UAS-Shits1 were from Dr. T. Sakai (Tokyo Metropolitan University). To remove possible modifiers and allow for comparisons in a common genetic background, we outcrossed all the strains into a control (w1118) background for at least five consecutive generations.
Hasegawa T., Tomita J., Hashimoto R., Ueno T., Kume S, & Kume K. (2017). Sweetness induces sleep through gustatory signalling independent of nutritional value in a starved fruit fly. Scientific Reports, 7, 14355.
+ Open protocol
+ Expand
2

Genetic Manipulation of Drosophila Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following strains were used in this study: fru-GAL4 7 , dsx-GAL4 11 (link) , UAS>stop>TNTin, UAS>stop>TNT 7 , UAS>stop>mCD8::GFP 9 (link) , FLP 335 and FLP 386 were generated as described in previous study 27 (link) , cha-GAL80 39 (link) , tsh-GAL80 from Julie Simpson, and Canton-S strain from the Bloomington Stock Center, Bloomington, IN. Oenocyte less (oe-) ies and their controls were generated as previously described 36 .
Jois S., Chan Y., Fernández M.d.l.P., Pujari N., Janz L.J., & Leung A.K. (2021). Sexually dimorphic mechanosensory neurons regulate copulation duration and persistence in male Drosophila.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!