Dade innovin prothrombin time reagent

Manufactured by Siemens

The Dade Innovin prothrombin time reagent is a laboratory diagnostic product manufactured by Siemens. The core function of this reagent is to assist in the measurement of prothrombin time, which is a common coagulation test used to evaluate the time it takes for blood to clot.

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Lab products found in correlation

Alexa fluor 647 conjugated human fibrinogen, by Thermo Fisher Scientific (2 mentions) Anti human cd61 antibody, by BD (1 mentions) Phe pro arg chloromethylketone ppack, by Prolytix (1 mentions) Sigmacote, by Merck Group (1 mentions) H gly pro arg pro oh gprp, by Merck Group (1 mentions) Anti human cd61, by BD (1 mentions) Collagen type 1 chrono par aggregation reagent, by Chrono-Log (1 mentions) D dimer elisa kit, by Abcam (1 mentions) Sigmacote siliconizing reagent, by Merck Group (1 mentions) Sylgard 184 silicone elastomer kit, by Dow (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Elisa kit, by MyBioSource (1 mentions) Enzygnost f1 2 monoclonal kit, by Siemens (1 mentions)

2 protocols using dade innovin prothrombin time reagent

Evaluating Fibrin Formation and Platelet Function

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The following reagents were obtained: Sigmacote (Millipore Sigma, Cat# SL2-100ML), collagen (type I; Chrono-Log, Cat# 385), Dade Innovin prothrombin time reagent (Siemens, Cat# B4212-40), corn trypsin inhibitor (CTI; Haematologic Technologies, Cat# CTI-01), fluorescently labeled fragment derived from α2-AP (Ac-GNQEQVSPLTLLKW-C[IAF], α2-APF, CPC Scientific), antihuman CD61 antibody (BD Biosciences, Cat# 555754), Alexa Fluor 647 Conjugated Human Fibrinogen (Life Technologies, Cat# F35200), H-Gly-Pro-Arg-Pro-OH (GPRP; Millipore Sigma, Cat# 03-34-0001), Phe-Pro-Arg-chloromethylketone (PPACK; Haematologic Technologies, Cat# FPRCK-01), 1,3,4,5-tetramethyl-2-([2-oxopropyl]thio) imidazolium chloride (T101; Zedria GmbH), Recombinant Human Tissue Plasminogen Activator Protein (tPA; Abcam, Cat# ab92637), and rhosin (Rho inhibitor; Millipore Sigma, CAS 1173671-63-0).

Liu Y., Crossen J., Stalker T.J, & Diamond S.L. (2023). Fluorescent peptide for detecting factor XIIIa activity and fibrin in whole blood clots forming under flow. Research and Practice in Thrombosis and Haemostasis, 8(1), 102291.

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Platelet Aggregation and Coagulation Assay

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The following reagents were obtained: anti-human CD61 (BD Biosciences, San Jose, CA), Alexa Fluor647-conjugated human fibrinogen (Life Technologies, Grand Island, NY), collagen type I Chrono-Par aggregation reagent (Chrono-log, Havertown, PA), CTI (corn trypsin inhibitor; Hematologic Technologies, Essex Junction, VT), Phe-Pro-Arg-chloromethylketone (PPACK; Hematologic Technologies, Essex Junction, VT), Dade Innovin prothrombin time reagent (Siemens, Malvern, PA), Enzygnost F1+2 monoclonal kit (Siemens Healthcare Diagnostic, Tarrytown, NY), D-dimer ELISA Kit (Abcam, Cambridge, MA), human FPA (fibrinopeptide-A), ELISA Kit (MyBioSource, San Diego, CA), EDTA (Sigma, St. Louis, MO), H-Gly-Pro-Arg-Pro-OH (EMD Chemicals, San Diego, CA), fluorescein Phe-Pro-Arg-chloromethylketone (fluorescein-PPACK; Hematologic Technologies, Essex Junction, VT), Sigmacote siliconizing reagent (Sigma, St. Louis, MO), and Sylgard 184 Silicone Elastomer kit (Dow Corning, Auburn, MI). O1A6 FXI antibody was a gift from Dr Andras Gruber (Department of Biomedical Engineering, Oregon Health and Science University). See the online-only Data Supplement for catalog numbers.

Zhu S., Chen J, & Diamond S.L. (2018). Establishing the Transient Mass Balance of Thrombosis: From Tissue Factor to Thrombin to Fibrin Under Venous Flow. Arteriosclerosis, Thrombosis, and Vascular Biology, 38(7), 1528-1536.

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