Enzyme activity assay kit for β n acetyl glucosaminidase

Manufactured by Merck Group

The Enzyme activity assay kit for β‐N‐acetyl‐glucosaminidase (NAG) is a laboratory tool used to measure the activity of the NAG enzyme. NAG is an enzyme involved in the breakdown of glycosaminoglycans. The assay kit provides the necessary reagents and protocols to quantify the enzymatic activity of NAG in samples.

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Lab products found in correlation

Formic acid, by Thermo Fisher Scientific (6 mentions) Acetonitrile, by Thermo Fisher Scientific (4 mentions) Methanol, by Thermo Fisher Scientific (4 mentions) Deep well 1 ml 96 well plate protein lobind, by Eppendorf (2 mentions) Recombinant mouse hexb protein his tag, by MyBioSource (2 mentions) Recombinant nag enzyme, by New England Biolabs (2 mentions) Instant pc kit, by Agilent Technologies (2 mentions) Tris hcl buffer, by Thermo Fisher Scientific (2 mentions) Dithiothreitol (dtt), by Thermo Fisher Scientific (2 mentions) Iodoacetamide iam, by Thermo Fisher Scientific (2 mentions) Sequencing grade modified trypsin, by Promega (2 mentions) Alcohol dehydrogenase, by Merck Group (1 mentions) Myoglobin, by Merck Group (1 mentions) Ammonium bicarbonate, by Avantor (1 mentions)

Close spelling variants of this product

Enzyme activity kit Activity Assay Kits

2 protocols using enzyme activity assay kit for β n acetyl glucosaminidase

Quantitative Proteomics Protocol

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Dithiothreitol (DTT) and iodoacetamide (IAM) were purchased from Thermo Pierce (Rockford, IL). Tris–HCl buffer and high‐performance liquid chromatography (HPLC) grade solvents, methanol, acetonitrile (ACN), formic acid (FA), were purchased from Thermo Fisher Scientific (Waltham, MA). The custom stable isotopic labeled (SIL) surrogate peptide (GILIDTSR, AQUA Ultimate grade) with 13C and 15N‐labeled arginine (R*), and nonlabeled surrogate peptide were synthesized from Thermo Fisher Scientific. Sequencing grade modified trypsin was purchased from Promega (Madison, WI). Ammonium bicarbonate and urea were purchased from JT Baker (Center Valley, PA). Deep well 1‐ml 96‐well plate (Protein LoBind) was purchased from Eppendorf (Hauppauge, NY). Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH) from yeast, carbonic anhydrase ii (CA2) from Bos Taurus, myoglobin (MB) from equine heart, phosphorylase‐b (PYGM) from rabbit muscle and enolase (ENL) from baker's yeast, and enzyme activity assay kit for β‐N‐acetyl‐glucosaminidase (NAG) were purchased from Sigma (St. Louis, MO). Recombinant NAG enzyme was from New England Biolabs (Beverly, MA). Instant PC kit was from (Agilent, formerly ProZyme, Palo Alto, CA).

Li X., An Y., Liao J., Xiao L., Swanson M., Martinez‐Fonts K., Pavon J.A., Sherer E.C., Jawa V., Wang F., Gao X., Letarte S, & Richardson D.D. (2021). Identification and characterization of a residual host cell protein hexosaminidase B associated with N‐glycan degradation during the stability study of a therapeutic recombinant monoclonal antibody product. Biotechnology Progress, 37(3), e3128.

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Quantitative Proteomics Using SIL Peptides

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Dithiothreitol (DTT) and iodoacetamide (IAM) were purchased from Thermo Pierce (Rockford, IL). Tris-HCl buffer and high-performance liquid chromatography (HPLC) grade solvents, methanol, acetonitrile (ACN), formic acid (FA), were purchased from Thermo Fisher Scientific (Waltham, MA). The custom stable isotopic labeled (SIL) surrogate peptide (GILIDTSR, AQUA Ultimate grade) with 13C and 15N labeled arginine (R*), and non-labeled surrogate peptide were synthesized from Thermo Fisher Scientific (Waltham, MA). The mAbs are from the pipeline of Merck & Co., Inc., Kenilworth, NJ, USA. Sequencing grade modified trypsin was purchased from Promega (Madison, WI). Ammonium bicarbonate and urea were purchased from JT Baker (Center Valley, PA). Deep well 1-mL 96 well plate (Protein LoBind) was purchased from Eppendorf (Hauppauge, NY). Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH) from yeast, carbonic anhydrase ii (CA2) from Bos Taurus, myoglobin (MB) from equine heart, phosphorylase-b (PYGM) from rabbit muscle and enolase (ENL) from baker's yeast, and enzyme activity assay kit for β-N-Acetyl-glucosaminidase (NAG) were purchased from Sigma (St. Louis, MO).
Recombinant NAG enzyme was from New England Biolabs (Beverly, MA). Instant PC kit was from Agilent (Palo Alto, CA).

Li X., An Y., Liao J., Xiao L., Swanson M.R., Martinez‐Fonts K., Pavon J.A., Sherer E.C., Jawa V., Gao X., Letarte S., & Richardson D.D. (2020). Identification and Characterization of a Residual Host Cell Protein Hexosaminidase B Associated with N-glycan Degradation During the Stability Study of a Therapeutic Recombinant Monoclonal Antibody Product.

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