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Pcdna3 flag crbn

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The pcDNA3-FLAG-CRBN is a plasmid vector that enables expression of the CRBN protein with a FLAG tag. The vector is designed for mammalian cell expression.

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Lab products found in correlation

Tet plko puro, by Addgene (2 mentions) Superscript 3 first strand kit, by Thermo Fisher Scientific (2 mentions) Quikchange 2 xl site directed mutagenesis kit, by Agilent Technologies (2 mentions)

2 protocols using pcdna3 flag crbn

1

Evaluating Tet-regulated shRNA Knockdown of CDK4/6 and CDC37

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tet-pLKO-puro (item #21915) and pcDNA3-FLAG-CRBN (item #107380) was purchased from Addgene. shCDK4 (TRCN0000018364 and TRCN0000010520), shCDK6 (TRCN0000010081: and TRCN0000000488) and shCDC37 (TRCN0000116633 and TRCN0000116632) (Supplementary Table 1) were subcloned into Tet-pLKO plasmid. Human CDK6 coding sequences were amplified from HEK293H cDNA using a SuperScript III First-Strand kit (Thermo Fisher). C-terminal V5-tagged CDK6 were subcloned using pcDNA3 as a backbone. shRNA-resistant form of V5-CDK6 and S178P V5-CDK6 were generated with a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent).
Wu X., Yang X., Xiong Y., Li R., Ito T., Ahmed T.A., Karoulia Z., Adamopoulos C., Wang H., Wang L., Xie L., Liu J., Ueberheide B., Aaronson S.A., Chen X., Buchanan S.G., Sellers W.R., Jin J, & Poulikakos P.I. (2021). Distinct CDK6 complexes determine tumor cell response to CDK4/6 inhibitors and degraders. Nature cancer, 2(4), 429-443.
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2

Evaluating Tet-regulated shRNA Knockdown of CDK4/6 and CDC37

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tet-pLKO-puro (item #21915) and pcDNA3-FLAG-CRBN (item #107380) was purchased from Addgene. shCDK4 (TRCN0000018364 and TRCN0000010520), shCDK6 (TRCN0000010081: and TRCN0000000488) and shCDC37 (TRCN0000116633 and TRCN0000116632) (Supplementary Table 1) were subcloned into Tet-pLKO plasmid. Human CDK6 coding sequences were amplified from HEK293H cDNA using a SuperScript III First-Strand kit (Thermo Fisher). C-terminal V5-tagged CDK6 were subcloned using pcDNA3 as a backbone. shRNA-resistant form of V5-CDK6 and S178P V5-CDK6 were generated with a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent).
Wu X., Yang X., Xiong Y., Li R., Ito T., Ahmed T.A., Karoulia Z., Adamopoulos C., Wang H., Wang L., Xie L., Liu J., Ueberheide B., Aaronson S.A., Chen X., Buchanan S.G., Sellers W.R., Jin J, & Poulikakos P.I. (2021). Distinct CDK6 complexes determine tumor cell response to CDK4/6 inhibitors and degraders. Nature cancer, 2(4), 429-443.
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