Sybr green based qpcr master mixes

Manufactured by Qiagen

SYBR Green-based qPCR master mixes are a ready-to-use solution for quantitative real-time PCR (qPCR) experiments. They contain all the necessary components, including SYBR Green I dye, DNA polymerase, and buffers, to perform qPCR reactions.

Automatically generated - may contain errors

Lab products found in correlation

Rneasy mini kit, by Qiagen (3 mentions)

Close spelling variants of this product

SYBR Green Master Mix (452 citations) SYBR Green (372 citations) SYBR Green Mix (45 citations) SYBR master mix (17 citations) QPCR SYBR Green Master Mix (7 citations) QPCR master mix (5 citations) SYBR green qPCR mix (2 citations) QPCR SYBR Green (2 citations)

3 protocols using sybr green based qpcr master mixes

Quantitative Analysis of Oncogene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was isolated from thyroid cancer cells using RNeasy Mini Kit (74104, Qiagen, Hilden, Gemany) according to the manufacturer's protocol. SYBR Green-based qPCR master mixes (330600) were obtained from Qiagen. Expression of Cyclin D1 (CCND1), c-Myc were examined using specific primers as following: CCND1, 5′-CCCTCGGTGTCCTACTTCAAA-3′ and 5′ CCAGGTTCCACTTGAGCTTGT-3′, c-Myc, 5′-CCTCAACGTTAGCTTCACCAA-3′ and 5′-TTTGATGAA GGTCTCGTCGTC-3′.

Liu M., Zhang Z., Wang H., Chen X, & Jin C. (2019). Activation of AMPK by metformin promotes renal cancer cell proliferation under glucose deprivation through its interaction with PKM2. International Journal of Biological Sciences, 15(3), 617-627.

+ Open protocol

+ Expand

Analysis of Glycolysis Regulators in Thyroid Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was isolated from thyroid cancer cells using RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol. SYBR Green-based qPCR master mixes were obtained from Qiagen. Expression of genes with an established role in regulation of glycolysis was examined using 'glycolysis' real-time PCR arrays (Qiagen). Expression of PKM (with specific isoforms being PKM1 and PKM2), glucose-6-phosphate dehydrogenase (G6PD), and fructose-1,6-biphosphatase 1 (F1) were examined using specific primers.

Bikas A., Jensen K., Patel A., Costello J J.r., McDaniel D., Klubo-Gwiezdzinska J., Larin O., Hoperia V., Burman K.D., Boyle L., Wartofsky L, & Vasko V. (2015). Glucose-deprivation increases thyroid cancer cells sensitivity to metformin. Endocrine-related cancer, 22(6).

+ Open protocol

+ Expand

Quantitative Analysis of Thyroid Cancer Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was isolated from thyroid cancer cells using the RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol. SYBR green-based qPCR master mixes were obtained from Qiagen. Quantitative RT-PCR screening of thyroid-specific genes (NKX2-1, SLC5A5 (sodium iodide symporter) and TG (thyroglobulin)) as well as genes with an established role in the regulation of epithelial-tomesenchymal transition (TwiST1, ViM (vimentin), CDH1 (E-Cadherin), CDH2 (N-Cadherin)) was performed using commercially available primers and probes from Qiagen.

Jensen K., Bikas A., Patel A., Kushchayeva Y., Costello J., McDaniel D., Burman K, & Vasko V. (2017). Nelfinavir inhibits proliferation and induces DNA damage in thyroid cancer cells. Endocrine-related cancer, 24(3).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!