Analytical reference standards of 29 acyl-carnitines (Sigma-Aldrich, Co., St. Louis, MO, USA) were employed for quality control of compounds in samples. We defined acyl-carnitines and major acyl-carnitine fractions as free-, short-, medium-, or long-chain acyl-carnitines according to the length of carbon chains in the molecular structure (i.e., free carnitine: C0, short-chain: C2-C5, medium-chain: C6-C12, long-chain: C14-C18)29 (link),30 (link). Deuterium-labeled carnitine and acyl-carnitines (NSK-B Set, Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA) were used as internal standards, including 2H9-carnitine (free carnitine, CN), 2H3-acetylcarnitine (C2), 2H3-propionylcarnitine (C3), 2H3-butyrylcarnitine (C4), 2H9-isovalerylcarnitine (C5), 2H3-octanoylcarnitine (C8), 2H9-myristoylcarnitine (C14), and 2H3-palmitoylcarnitine (C16). HPLC-grade ammonium acetate was purchased from Sigma-Aldrich (St. Louis, MO, USA); HPLC-grade formic acid and methanol were supplied by Thermo Fisher Scientific (Waltham, MA, USA); pure water was purchased from Wahaha Co., Ltd. (Hangzhou, China).
2h3 propionylcarnitine
Manufactured by Cambridge Isotopes
Sourced in United States
2H3-propionylcarnitine is a stable isotope-labeled compound used for analytical and research purposes. It is a deuterated form of propionylcarnitine, which is involved in the metabolism of certain fatty acids and amino acids. This product can be used as an internal standard or tracer in analytical techniques such as mass spectrometry.
Automatically generated - may contain errors
Lab products found in correlation
2h9 carnitine, by Cambridge Isotopes (2 mentions)
2h3 acetylcarnitine, by Cambridge Isotopes (2 mentions)
2h3 butyrylcarnitine, by Cambridge Isotopes (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Hplc grade formic acid, by Thermo Fisher Scientific (1 mentions)
Hplc grade ammonium acetate, by Merck Group (1 mentions)
2h9 isovalerylcarnitine, by Cambridge Isotopes (1 mentions)
2h3 octanoylcarnitine, by Cambridge Isotopes (1 mentions)
2h3 palmitoylcarnitine, by Cambridge Isotopes (1 mentions)
2h9 myristoylcarnitine c14, by Cambridge Isotopes (1 mentions)
Agilent 6460 triple quadrupole mass spectrometer, by Agilent Technologies (1 mentions)
2 protocols using 2h3 propionylcarnitine
1
Analytical Reference Standards for Acyl-Carnitines
2
Acylcarnitine and Amino Acid Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma acylcarnitines and amino acids were measured and analyzed by liquid chromatography and tandem mass spectrometry (LC-MS/MS) on an Agilent 6460 Triple Quadrupole Mass Spectrometer by the Mount Sinai Biochemical Genetic Testing Lab (now Sema4), as previously described (Le et al. 2014; Leandro et al. 2019; Ranea-Robles et al. 2020) . The L-carnitine internal standard ( 2 H 9 -carnitine) and the acylcarnitine internal standard mix containing 2 H 9 -carnitine, 2 H 3 -acetylcarnitine (C2), 2 H 3 -propionylcarnitine (C3), 2 H 3 -butyrylcarnitine (C4), 2 H 9 -isovalerylcarnitine (C5), 2 H 3 -octanoylcarnitine (C8), 2 H 9myristoylcarnitine (C14), and 2 H 3 -palmitoylcarnitine (C16), were from Cambridge Isotope Laboratories (Tewksbury, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!