The total RNA was isolated from the hippocampus region. We used 1% agarose gel electrophoresis and NanoDrop ND-1000 (Thermo Fisher Scientific, Waltham, MA, USA) for sample quantification and qualification. Subsequently, we amplified and transcribed the total RNA into fluorescent cRNA using the Agilent Array platform, followed by hybridization using the Rat LncRNA Microarray v2.0 (Arraystar, Rockville, USA, including 13,611 lncRNAs and 24,626 protein-coding mRNAs) overnight. After that, the microarray was scanned using an Agilent DNA Microarray ScannerG2505C (Agilent Technologies, Santa Clara, CA, USA). We analyzed and processed the acquired images using the Agilent Feature Extraction (v11) and GeneSpringGXv12.1 (Agilent Technologies). Standard routine analyses, including probe summarization (RMA), quality control, and probe annotation, were performed. The differentially expressed lncRNA (DE lncRNAs) and genes (DEGs) between groups were identified using the t-test with the criteria of fold change > 1.5 and p <.05.
Rat lncrna microarray v2
Manufactured by Arraystar
Sourced in United States
The Rat LncRNA Microarray v2.0 is a high-throughput laboratory equipment designed for the analysis of long non-coding RNA (lncRNA) expression in rat samples. The microarray platform provides comprehensive coverage of the rat transcriptome, enabling researchers to study the differential expression of lncRNAs under various experimental conditions.
Automatically generated - may contain errors
Lab products found in correlation
Agilent array platform, by Agilent Technologies (3 mentions)
Agilent dna microarray scanner g2505c, by Agilent Technologies (3 mentions)
Agilent feature extraction v11, by Agilent Technologies (3 mentions)
Genespring gx v12, by Agilent Technologies (3 mentions)
Nanodrop nd 1000, by Thermo Fisher Scientific (1 mentions)
3 protocols using rat lncrna microarray v2
1
Transcriptional Profiling of Hippocampal lncRNAs
2
Hippocampal lncRNA and mRNA Expression Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The total RNA was isolated from the hippocampus region. We used 1% agarose gel electrophoresis and NanoDrop ND-1000 (Thermo Fisher Scienti c, Waltham, MA, USA) for sample quanti cation and quali cation. Subsequently, we ampli ed and transcribed the total RNA into uorescent cRNA using the Agilent Array platform, followed by hybridization using the Rat LncRNA Microarray v2.0 (Arraystar, Rockville, USA, including 13,611 lncRNAs and 24,626 protein-coding mRNAs) overnight. After that, the microarray was scanned using an Agilent DNA Microarray ScannerG2505C (Agilent Technologies, Santa Clara, CA, USA). We analyzed and processed the acquired images using the Agilent Feature Extraction (v11) and GeneSpringGXv12.1 (Agilent Technologies). Standard routine analyses, including probe summarization (RMA), quality control, and probe annotation, were performed. The differentially expressed lncRNA (DE lncRNAs) and genes (DEGs) between groups were identi ed using the t-test with the criteria of fold change > 1.5 and p <0 .05.
3
Hippocampal lncRNA and mRNA Expression Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The total RNA was isolated from the hippocampus region. We used 1% agarose gel electrophoresis and NanoDrop ND-1000 (Thermo Fisher Scienti c, Waltham, MA, USA) for sample quanti cation and quali cation. Subsequently, we ampli ed and transcribed the total RNA into uorescent cRNA using the Agilent Array platform, followed by hybridization using the Rat LncRNA Microarray v2.0 (Arraystar, Rockville, USA, including 13,611 lncRNAs and 24,626 protein-coding mRNAs) overnight. After that, the microarray was scanned using an Agilent DNA Microarray ScannerG2505C (Agilent Technologies, Santa Clara, CA, USA). We analyzed and processed the acquired images using the Agilent Feature Extraction (v11) and GeneSpringGXv12.1 (Agilent Technologies). Standard routine analyses, including probe summarization (RMA), quality control, and probe annotation, were performed. The differentially expressed lncRNA (DE lncRNAs) and genes (DEGs) between groups were identi ed using the t-test with the criteria of fold change > 1.5 and p <0 .05.
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