Formalin xed, para n-embedded intestinal (cecal, ileum) tissue sections (4 µm) were incubated overnight at 4 °C with a primary antibody targeting the mouse antigens 1) histamine receptor 2 (AHR-002, Alomone labs, Israel), 2) Tryptase (ab2378, Abcam, USA), after antigen retrieval according to the manufacturer's instructions. Samples were washed and subsequently incubated with secondary antibody for 45 min at RT (Histo ne simple stain anti-rabbit, 414341F, Nacalai, USA and Alexa uor® 647, ab150131, Abcam, USA). Sections were counter-stained either with the diaminobenzidine substrate kit (Nacalai, USA) followed by haematoxylin or sections were stained with 6-diamidino-2-phenylindole (DAPI, Thermo scher, USA) as previously described for visualization of cell nuclei.
Alexa uor 647
Manufactured by Abcam
Sourced in United States
Alexa Fluor® 647 is a fluorescent dye that can be used to label biomolecules for detection and imaging applications. It has an excitation maximum at 650 nm and an emission maximum at 665 nm, making it suitable for detection with commonly used fluorescence instrumentation.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using alexa uor 647
1
Immunohistochemical Analysis of Intestinal Tissue
2
Immunohistochemical Analysis of Intestinal Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Formalin xed, para n-embedded intestinal (cecal, ileum) tissue sections (4 µm) were incubated overnight at 4 °C with a primary antibody targeting the mouse antigens 1) histamine receptor 2 (AHR-002, Alomone labs, Israel), 2) Tryptase (ab2378, Abcam, USA), after antigen retrieval according to the manufacturer's instructions. Samples were washed and subsequently incubated with secondary antibody for 45 min at RT (Histo ne simple stain anti-rabbit, 414341F, Nacalai, USA and Alexa uor® 647, ab150131, Abcam, USA). Sections were counter-stained either with the diaminobenzidine substrate kit (Nacalai, USA) followed by haematoxylin or sections were stained with 6-diamidino-2-phenylindole (DAPI, Thermo scher, USA) as previously described for visualization of cell nuclei.
3
Immunohistochemical Analysis of Intestinal Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Formalin xed, para n-embedded intestinal (cecal, ileum) tissue sections (4 µm) were incubated overnight at 4 °C with a primary antibody targeting the mouse antigens 1) histamine receptor 2 (AHR-002, Alomone labs, Israel), 2) Tryptase (ab2378, Abcam, USA), after antigen retrieval according to the manufacturer's instructions. Samples were washed and subsequently incubated with secondary antibody for 45 min at RT (Histo ne simple stain anti-rabbit, 414341F, Nacalai, USA and Alexa uor® 647, ab150131, Abcam, USA). Sections were counter-stained either with the diaminobenzidine substrate kit (Nacalai, USA) followed by haematoxylin or sections were stained with 6-diamidino-2-phenylindole (DAPI, Thermo scher, USA) as previously described for visualization of cell nuclei.
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