Middlebook oadc

Potato dextrose agar, Middlebook 7H9 broth, polysorbate 80 and Middlebook OADC were purchased from BD (Franklin Lakes, NJ, USA). All other chemicals including organic solvents were purchased from Wako Pure Chemical Industries (Osaka, Japan).

General Experimental Procedures Specific rotations were assessed with a JASCO P-2300 digital polarimeter (JASCO, Ltd., Tokyo, Japan). UV spectra were measured on a U-3310 UV-Vis spectrophotometer (Hitachi, Ltd., Tokyo, Japan) and IR spectra on a PerkinElmer, Inc. Spectrum One Fourier transform infrared spectrometer (PerkinElmer, Inc., Waltham, MA, U.S.A.). ECD spectra were measured with a J-720 spectrometer (JASCO). NMR spectra were recorded on a JEOL JNM-AL-400 NMR spectrometer (400 MHz for 1 H and 100 MHz for 13 C) in CDCl 3 (δ H 7.24, δ C 77.0). FAB-MS and HR-FAB-MS were performed using a JMS-MS 700 mass spectrometer (JEOL, Tokyo, Japan). Preparative HPLC was performed with a Hitachi L-6200 system (Hitachi, Ltd.).
Materials Middlebook 7H9 broth, polysorbate 80, and Middlebook OADC were purchased from BD (Franklin Lakes, NJ, U.S.A.). All other chemicals including organic solvents were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). ). 1 (link) H-and 13 C-NMR (CDCl 3 ), see Table 1.