Blood samples were obtained at the time of hospital admission. Complete blood counts were performed utilizing a Sysmex XE‐5000 analyzer (Sysmex). Plasma BNP was measured with the AIA‐2000 enzymatic immunoassay analyzer (TOSOH). Other biomarkers were measured using a LABOSPECT 008 autoanalyzer (Hitachi Co.). The estimated glomerular filtration rate (eGFR) was calculated by the Modification of Diet in Renal Disease formula (Matsuo et al., 2009 (link)). Echocardiographic examination was performed by an experienced sonographer using Vivid E9 with XD clear (GE Healthcare). The images were recorded in a console and analyzed offline. Left ventricular ejection fraction was calculated using the modified Simpson's rule.
Xe 5000 analyzer
Manufactured by Sysmex
Sourced in Japan
The XE-5000 analyzer is a flagship hematology instrument manufactured by Sysmex. It is designed to perform comprehensive blood cell analysis, providing detailed information on various blood cell parameters. The XE-5000 utilizes advanced technology to deliver accurate and reliable results for clinical diagnostic purposes.
Automatically generated - may contain errors
Lab products found in correlation
Labospect 008 autoanalyzer, by Hitachi (2 mentions)
Cobas 6000 analyzer, by Roche (2 mentions)
Xd clear, by GE Healthcare (1 mentions)
Vivid e9, by GE Healthcare (1 mentions)
Cobas integra 800, by Roche (1 mentions)
Cobas t 711, by Roche (1 mentions)
Cobas t511, by Roche (1 mentions)
Latex agglutination test, by Denka Seiken (1 mentions)
Elisa kit, by Assaypro (1 mentions)
Vista analyzer, by Siemens (1 mentions)
Cobas 6000, by Roche (1 mentions)
Dimension xpand, by Siemens (1 mentions)
Cobas 8000 modular analyzer series, by Roche (1 mentions)
Vantera analyzer, by LabCorp (1 mentions)
10 protocols using xe 5000 analyzer
1
Biomarker Assessment in Hospital Admissions
2
Comprehensive Coagulation Profile Evaluation
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All patients agreed to provide samples to conduct TEG assays, PLT, and coagulation tests. All samples were collected at the appropriate time and analyzed within 2 hours after collection. The citrated blood samples were tested and assayed using Sysmex XE5000 analyzer (Sysmex, Tokyo, Japan). Heparin-anticoagulated samples underwent chemistry tests and assayed using COBAS INTEGRA 800 biochemical analyzer (Roche, Basel, Switzerland). aPTTs and PTs were performed using the Sysmex CS2100i coagulometer with Innovin (Sysmex Europe GmbH, Norderstedt, Germany).
3
Comprehensive Hematological and Inflammatory Profiling
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The following parameters were measured using EDTA anti-coagulated whole blood:
Neutrophil granulocytes (*109/L), RBCs (*1012/L), thrombocytes (*109/L), relative numbers of eosinophils (%), leukocytes (*109/L), RDW (%), Hct (%), Hb concentration (g/dL), MCH (pg), MCHC (g/dL), MCV (fl), C-reactive protein (CRP) (mg/L), ferritin (µg/L), D-dimers (mg/IU), and fibrinogen concentration (g/L). Parameters were determined and analyzed by clinical standards using the Sysmex XE-5000 analyzer (Sysmex, Hamburg, Germany) system (based on resistance measurement principle (impedance measurement, Coulter measurement principle), photometric measurement, differentiation in a flow cell by means of laser via VCSn technology (volume, conductivity, scatter)) as well as ECLIA (Roche Immunoassay Analyzer Cobas 8000, Cobas t 711 and Cobas t 511, Basel, Switzerland) and ELISA measurements.
Neutrophil granulocytes (*109/L), RBCs (*1012/L), thrombocytes (*109/L), relative numbers of eosinophils (%), leukocytes (*109/L), RDW (%), Hct (%), Hb concentration (g/dL), MCH (pg), MCHC (g/dL), MCV (fl), C-reactive protein (CRP) (mg/L), ferritin (µg/L), D-dimers (mg/IU), and fibrinogen concentration (g/L). Parameters were determined and analyzed by clinical standards using the Sysmex XE-5000 analyzer (Sysmex, Hamburg, Germany) system (based on resistance measurement principle (impedance measurement, Coulter measurement principle), photometric measurement, differentiation in a flow cell by means of laser via VCSn technology (volume, conductivity, scatter)) as well as ECLIA (Roche Immunoassay Analyzer Cobas 8000, Cobas t 711 and Cobas t 511, Basel, Switzerland) and ELISA measurements.
4
Analyzing RDW and Biomarkers in Patients
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Complete blood counts for RDW values were performed utilizing a Sysmex XE-5000 analyzer (Sysmex, Kobe, Japan). The normal range of RDW in this system is 11.5-14.5%. Blood samples were obtained at the time of hospital admission and at discharge. Changes in RDW were calculated as the mean difference between RDW values on admission and those at the time of hospital discharge. In some analyses, we divided the patients into two groups according to whether they had higher or lower than the normal range of RDW levels on admission. Plasma B-type natriuretic peptide (BNP) was measured with the AIA-2000 enzymatic immunoassay analyzer (TOSOH, Tokyo, Japan). Other biomarkers were measured using a LABOSPECT 008 autoanalyzer (Hitachi Co., Tokyo, Japan). Estimated glomerular filtration rate (eGFR) was calculated by the Modification of Diet in Renal Disease (MDRD) formula [16] .
5
Blood Cell Analysis and Inflammation Markers
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White blood cell (WBC), neutrophil (NEU), lymphocyte (LY) and platelet (PLT) were collected from the blood acquired during a routine examination, and the results were detected using a Sysmex XE5000 analyzer (Sysmex, Japan). The NLR was defined as the ratio of NEUs and LYs, and the PLR was defined as the ratio of PLTs and LYs. Serum C-reactive protein (CRP) was determined by a latex-enhanced homogeneous immunoassay on a Hitachi LAS008 analyzer (Hitachi, Japan).
6
Biomarker Assessment in Fasted Subjects
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Blood samples for the measurement of the ZAG levels, complete blood count, and biochemical analysis were obtained from the antecubital vein of the subjects after an 8-h fast. Complete blood count (CBC) and biochemical analysis were done in the central laboratory in our hospital, which are accredited by College of American Pathologists (CAP) and ISO15189. The CBC samples were analyzed on the Sysmex XE5000 analyzer (Kakogawa, Japan). Biochemical analysis was performed on the “Beckman Coulter” automated chemistry analyzer AU5800 (Shizuoka-Ken, Japan). For the ZAG study, the blood was then immediately centrifuged. The plasma samples obtained after centrifugation were then frozen at −70 °C until analysis. Plasma ZAG levels were measured by the commercial enzyme-linked immunosorbent assay (ELISA) kits (BioVendor, Brno, Czech). Plasma tumor-necrosis factor alpha (TNF-α) levels were measured by the commercial enzyme-linked immunosorbent assay (ELISA) kits (Assaypro LLC, Saint Charles, MO). Plasma CRP levels were measured by the latex agglutination test (Denka Seiken, Gosen, Niigata, Japan).
7
Comprehensive Blood and Serum Biomarker Analysis
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Hemoglobin, hematocrit, mean corpuscular volume (MCV), absolute reticulocyte count, and percentage reticulocyte count were measured in EDTA-anticoagulated whole blood using a Sysmex XE-5000 analyzer (Chicago, IL). Glucose, total bilirubin, direct bilirubin, liver enzymes, blood urea nitrogen, creatinine, vitamin B12, and folate were measured in serum using the Vista analyzer (Siemens Healthcare, Malvern, PA). Insulin was measured in serum on the Cobas 6000 instrument (Roche Diagnostics, Indianapolis, IN). Iron, transferrin, and ferritin were analyzed in serum on the Immulite XP and analyzer (Siemens Healthcare), and urinary microalbumin was measured using the Dimension Xpand (Siemens Healthcare).
8
Septic Shock Hematological Markers Study
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This retrospective cohort study was carried out in a tertiary medical center of over 1800 beds disserving a large area in the north of China.
From January to December 2012, all patients diagnosed with septic shock were evaluated for inclusion in the study. Exclusion criteria were as follows: patients with concomitant hematological diseases (hematological malignancies, autoimmune thrombocytopenic purpura, reactive thrombocytosis, and hypersplenism), patients receiving platelets or fresh frozen plasma, and pregnant or breastfeeding women. All medical charts were independently reviewed by two physicians: if there was discrepancy between them regarding patient inclusion, all other authors were consulted to reach agreement.
The complete blood counts (CBCs) performed on the first five days after admission, on the penultimate and last day of hospital stay were reviewed. The CBC included: the PLT count, MPV, PDW, PLCR, PCT, HCT and WBC count. The CBCs were performed using a Sysmex XE 5000 analyzer (Japan).The normal range of PLT, MPV, PDW, PLCR, and PCT were 100–300×109/L, 7–13 fl, 9–17%, 13–43%, and 0.11–0.28% respectively. Lactate, procalcitonin and calcium levels as well as hepatic and renal functions, performed on the same days as the CBC, were also reviewed.
From January to December 2012, all patients diagnosed with septic shock were evaluated for inclusion in the study. Exclusion criteria were as follows: patients with concomitant hematological diseases (hematological malignancies, autoimmune thrombocytopenic purpura, reactive thrombocytosis, and hypersplenism), patients receiving platelets or fresh frozen plasma, and pregnant or breastfeeding women. All medical charts were independently reviewed by two physicians: if there was discrepancy between them regarding patient inclusion, all other authors were consulted to reach agreement.
The complete blood counts (CBCs) performed on the first five days after admission, on the penultimate and last day of hospital stay were reviewed. The CBC included: the PLT count, MPV, PDW, PLCR, PCT, HCT and WBC count. The CBCs were performed using a Sysmex XE 5000 analyzer (Japan).The normal range of PLT, MPV, PDW, PLCR, and PCT were 100–300×109/L, 7–13 fl, 9–17%, 13–43%, and 0.11–0.28% respectively. Lactate, procalcitonin and calcium levels as well as hepatic and renal functions, performed on the same days as the CBC, were also reviewed.
9
Blood Sample Processing for Analysis
10
Metabolic and Hematological Biomarkers
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Disposition Index (DI)25 (link) :
Glucose, insulin, liver enzymes were analyzed in serum with Roche Cobas 6000 analyzer (Roche Diagnostics, Indianapolis, Indiana, USA).
Cholesterol, triglycerides (TG) and direct high-density lipoprotein cholesterol (HDL-C) were measured enzymatically on the Cobas 6000 analyzer (Roche Diagnostics). Low-density lipoprotein cholesterol (LDL-C) was calculated using the Friedewald equation.26 (link) LDL particle number, and HDL and LDL size were measured by nuclear magnetic resonance (NMR) on the Vantera analyzer, using the Lp3 algorithm (LabCorp).
Hemoglobin, hematocrit, white cells and mean corpuscular volume (MCV) were measured in EDTA-anticoagulated whole blood using a Sysmex XE-5000 analyzer (Chicago, Illinois, USA). Iron, transferrin and ferritin were measured in serum (Roche Cobas 6000 analyzer).
Glucose, insulin, liver enzymes were analyzed in serum with Roche Cobas 6000 analyzer (Roche Diagnostics, Indianapolis, Indiana, USA).
Cholesterol, triglycerides (TG) and direct high-density lipoprotein cholesterol (HDL-C) were measured enzymatically on the Cobas 6000 analyzer (Roche Diagnostics). Low-density lipoprotein cholesterol (LDL-C) was calculated using the Friedewald equation.26 (link) LDL particle number, and HDL and LDL size were measured by nuclear magnetic resonance (NMR) on the Vantera analyzer, using the Lp3 algorithm (LabCorp).
Hemoglobin, hematocrit, white cells and mean corpuscular volume (MCV) were measured in EDTA-anticoagulated whole blood using a Sysmex XE-5000 analyzer (Chicago, Illinois, USA). Iron, transferrin and ferritin were measured in serum (Roche Cobas 6000 analyzer).
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