RNA was prepared from frozen samples using Zymo Research Quick RNA MIcro Prep Kit # R1050, and cleaned using Zymo Research RNA Clean and Concentrator, #R1015. Samples were analyzed on an Agilent QC 2100 Bioanalyzer to determine quality prior to library preparation. cDNA was prepared used the Nugen Ovation RNA Sequencing System V2, #7102 and cleaned using the QIAGEN QIAquick PCR purification kit, #28104, as recommended in the protocol and analyzed on the Agilent QC Bioanalyzer. If needed, samples were size-selected using Zymo Research Select-A-Size DNA Clean and Concentrator #D4080 prior to barcoding. Final library was prepared using NEB NEBNext Ultra II DNA Library Prep Kit #27645 and barcoded using NEBNext Multiplex Oligos for Illumina #E6609S. All magnetic bead purification was accomplished using BullDogBio CleanNGS RNA and DNA Spri Beads #CNGS005. Samples were then analyzed on the Agilent QC 2100 Bioanalyzer to determine the concentration of each sample prior to determine dilution prior to sequencing. On average, 12 million 2×150 bp reads (Illumina Nextseq 500) were sequenced for each library.
Select a size dna clean and concentrator
Manufactured by Zymo Research
The Select-A-Size DNA Clean and Concentrator is a lab equipment product designed to purify and concentrate DNA samples. It enables users to selectively recover DNA fragments of specific sizes from a sample, allowing for efficient downstream processing and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Nextseq 500, by Illumina (2 mentions)
Nebnext ultra 2 dna library prep kit, by Illumina (2 mentions)
Quick rna microprep kit, by Zymo Research (2 mentions)
Rna clean and concentrator, by Zymo Research (2 mentions)
Ovation rna sequencing system v2, by Qiagen (2 mentions)
Qiaquick pcr purification kit, by Agilent Technologies (2 mentions)
Agilent qc bioanalyzer, by Agilent Technologies (2 mentions)
Miseq instrument, by Illumina (1 mentions)
Tapestation, by Agilent Technologies (1 mentions)
Quick 16s ngs library prep kit, by Zymo Research (1 mentions)
3 protocols using select a size dna clean and concentrator
1
RNA Sequencing Library Preparation
2
16S rRNA Sequencing with Zymo Kit
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Bacterial 16S ribosomal RNA gene-targeted sequencing was performed using the Quick-16S NGS Library Prep Kit (Zymo Research). The bacterial 16S primers amplified the V1–V2 or V3–V4 regions of the 16S rRNA gene. Primers were custom-designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity.
The sequencing library was prepared using a process in which PCR reactions were performed in real-time PCR format to control cycles and prevent/limit PCR chimera formation. The final PCR products were quantified by qPCR fluorescence readings and pooled together based on equal molarity. The pooled library was cleaned up with the Select-a-Size DNA Clean and Concentrator (Zymo Research) and then quantified with TapeStation (Agilent Technologies) on the Qubit platform. The final library was sequenced on an Illumina MiSeq instrument (RRID:SCR_016379) using the v3 reagent kit (600 cycles). The sequencing was performed with >10% PhiX spike-in.
The sequencing library was prepared using a process in which PCR reactions were performed in real-time PCR format to control cycles and prevent/limit PCR chimera formation. The final PCR products were quantified by qPCR fluorescence readings and pooled together based on equal molarity. The pooled library was cleaned up with the Select-a-Size DNA Clean and Concentrator (Zymo Research) and then quantified with TapeStation (Agilent Technologies) on the Qubit platform. The final library was sequenced on an Illumina MiSeq instrument (RRID:SCR_016379) using the v3 reagent kit (600 cycles). The sequencing was performed with >10% PhiX spike-in.
3
RNA Sequencing Library Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RNA was prepared from frozen samples using Zymo Research Quick RNA MIcro Prep Kit # R1050, and cleaned using Zymo Research RNA Clean and Concentrator, #R1015. Samples were analyzed on an Agilent QC 2100 Bioanalyzer to determine quality prior to library preparation. cDNA was prepared used the Nugen Ovation RNA Sequencing System V2, #7102 and cleaned using the QIAGEN QIAquick PCR purification kit, #28104, as recommended in the protocol and analyzed on the Agilent QC Bioanalyzer. If needed, samples were size-selected using Zymo Research Select-A-Size DNA Clean and Concentrator #D4080 prior to barcoding. Final library was prepared using NEB NEBNext Ultra II DNA Library Prep Kit #27645 and barcoded using NEBNext Multiplex Oligos for Illumina #E6609S. All magnetic bead purification was accomplished using BullDogBio CleanNGS RNA and DNA Spri Beads #CNGS005. Samples were then analyzed on the Agilent QC 2100 Bioanalyzer to determine the concentration of each sample prior to determine dilution prior to sequencing. On average, 12 million 2×150 bp reads (Illumina Nextseq 500) were sequenced for each library.
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