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2 protocols using lpc 20 0

1

Obtaining Lysophosphatidylcholine Lipids

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Lysophosphatidylcholines (LPC
16:0, LPC 18:0, LPC 18:1, LPC 18:2, LPC 20:0, and LPC 22:0) were obtained
from Avanti Polar Lipids (Alabaster, AL). All solvents and organic
reagents were of the highest grade commercially available.
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2

Lipid Profiling of Biological Samples

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The reference standards of LPC(13:0) (internal standard, IS), LPC(14:0), LPC(16:0), LPC(17:0), LPC(18:1) and LPC(20:0) were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA). Mass spectrometry grade-methanol and acetonitrile were obtained from Merck Inc. (Darmstadt, HE, Germany). Analytical reagent-chloroform and HPLC grade-formic acid were purchased from Xilong Technology Co., Ltd. (Shantou, China). Phosphate buffer saline (PBS) was supplied by Servicebio Technology Co., Ltd. (Wuhan, China). Bovine serum albumin (BSA) was provided by Energy Chemical Technology Co., Ltd. (Shanghai, China). CH and APAP were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Isoflurane was supplied by RWD Life Science CO., Ltd. (Shenzhen, China)
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