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Dylight fluor coupled secondary antibodies

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DyLight Fluor-coupled secondary antibodies are a type of fluorescent-labeled secondary antibody products designed for use in various immunodetection techniques. They provide a simple and reliable way to detect and visualize target proteins or molecules in biological samples.

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Lab products found in correlation

Mab1125, by R&D Systems (2 mentions) Af3628, by R&D Systems (2 mentions) Alexa fluor 555 conjugated 70 kda dextran, by Thermo Fisher Scientific (2 mentions) P0799, by Merck Group (2 mentions)

2 protocols using dylight fluor coupled secondary antibodies

1

Vascular Perfusion Analysis in Embryos

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E12.5 mutant and control embryos underwent cardiac perfusion with Alexa Fluor 555-conjugated 70 kDa dextran (50 μL of 2.5 mg/mL in PBS; Invitrogen) using a pulled glass pipette with mouth connector and tubing (Sigma, P0799) in the course of the 15 to 20 minutes when a visible heart beat could be observed. Fluorescent visualization of the entire vasculature confirmed the perfusion. Tracer was left circulating for 10 minutes at room temperature, embryos were decapitated, their heads fixed in 4% paraformaldehyde overnight at 4°C, cryosectioned and stained as described above. Staining was performed with the following primary antibodies; CD31/PECAM1 (R&D systems, AF3628) 1:1000 and monoclonal TER-119 (R&D Systems, MAB1125) 1:250 followed by a 2 hours incubation at room temperature with DyLight Fluor-coupled secondary antibodies (1:1000, Thermo Scientific Pierce).
Jensen L.D., Hot B., Ramsköld D., Germano R.F., Yokota C., Giatrellis S., Lauschke V.M., Hubmacher D., Li M.X., Hupe M., Arnold T.D., Sandberg R., Frisén J., Trusohamn M., Martowicz A., Wisniewska-Kruk J., Nyqvist D., Adams R.H., Apte S.S., Vanhollebeke B., Stenman J.M, & Kele J. (2019). Disruption of the Extracellular Matrix Progressively Impairs Central Nervous System Vascular Maturation Downstream of β-Catenin Signaling. Arteriosclerosis, Thrombosis, and Vascular Biology, 39(7), 1432-1447.
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2

Vascular Network Visualization in Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols
E12.5 mutant and control embryos underwent cardiac perfusion with Alexa Fluor 555-conjugated 70 kDa dextran (50 μl of 2.5 mg/ml in PBS;Invitrogen) using a pulled glass pipette with mouth connector and tubing (Sigma, P0799) in the course of the 15–20 min when a visible heart beat could be observed. Fluorescent visualization of the entire vasculature confirmed the perfusion. Tracer was left circulating for 10 min at room temperature, embryos were decapitated, their heads fixed in 4% PFA overnight at 4°C, cryosectioned and stained as described above. Staining was performed with the following primary antibodies; CD31/PECAM1 (R&D systems, AF3628) 1:1000 and monoclonal TER-119 (R&D systems, MAB1125) 1:250 followed by a 2h incubation at room temperature with DyLight Fluor-coupled secondary antibodies (1:1000, Thermo Scientific Pierce).
Jensen L.D., Hot B., Ramsköld D., Germano R.F., Yokota C., Giatrellis S., Lauschke V.M., Hubmacher D., Li M.X., Hupe M., Arnold T.D., Sandberg R., Frisén J., Trusohamn M., Martowicz A., Wisniewska-Kruk J., Nyqvist D., Adams R.H., Apte S.S., Vanhollebeke B., Stenman J.M, & Kele J. (2019). Disruption of the Extracellular Matrix Progressively Impairs Central Nervous System Vascular Maturation Downstream of β-Catenin Signaling. Arteriosclerosis, Thrombosis, and Vascular Biology, 39(7), 1432-1447.
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