Torin

Manufactured by Axon Medchem

Sourced in United States

Torin is a versatile laboratory equipment used for various applications in scientific research. It is a highly precise and reliable instrument designed to perform a wide range of tasks, including sample preparation, analysis, and processing. The core function of Torin is to provide accurate and consistent control over temperature, pressure, and other key parameters, ensuring the integrity and reproducibility of experimental results.

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Lab products found in correlation

2 protocols using torin

Primary Hepatocyte Inhibitor Treatment

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For the treatment of the primary hepatocytes from C57BL/6N mice with the inhibitors, we used cells from one mice and treated them with final concentrations of 10 µM cyclopamine (LC Laboratories, Woburn, MA, USA), 50 nM rapamycin (Sigma-Aldrich, St. Louis, MO, USA) or 5 µM Torin (Axon Medchem LLC, Reston, VA, USA) diluted in dimethyl sulfoxide (DMSO) (Carl Roth GmbH + Co. KG, Karlsruhe, Germany) were added to the serum-free media. For combinatory treatment with cyclopamine, higher concentrated inhibitors were added to reach the final concentration with the same amount of DMSO. As controls we used media without any addition, to detect possible effects of the DMSO, and media added with the same amount of DMSO as was added with the inhibitors. This experiment was repeated 3–5 times with different male and female mice.

Spormann L., Rennert C., Kolbe E., Ott F., Lossius C., Lehmann R., Gebhardt R., Berg T, & Matz-Soja M. (2020). Cyclopamine and Rapamycin Synergistically Inhibit mTOR Signalling in Mouse Hepatocytes, Revealing an Interaction of Hedgehog and mTor Signalling in the Liver. Cells, 9(8), 1817.

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Innate Immune Stimuli Combination Protocol

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300 ng/mL Pam3Cys-SKKKK (EMC-microcollections L2000), 20 μg/mL LMW polyinosinicpolycytidylic acid (InvivoGen tlrl-picw), 100 ng/mL lipopolysaccharides E. coli 055:B5 (Sigma L6529), 5 μg/mL FLA (B. subtilis) ~32 kDa Gram-positive flagellin (InvivoGen FLA-BS), 10 μg/mL R848 imidazoquinoline compound (InvivoGen tlrl-r848), 5 μM CpG (ODN 1826) unmethylated CpG dinucleotides (InvivoGen tlrl-1826), 10 μg/mL muramyl dipeptide (InvivoGen tlrl-mdp), 10 μg/mL synthetic analog of trehalose-6,6- dimycolate (TDB, Trehalose-6,6-dibehenate) (InvivoGen tlrl-tdb), MOI ~10 Sendai virus (Sendai strain: Cantell) (ATCC VR-907), 5 μg/mL zymosan (S. cerevisiae) (Sigma Z4250), 20 μg/mL HT-DNA (herring testis DNA) (Trevigen). DMSO (Sigma), 10 μM forskolin (Cayman Chemical), 25 μM tert-Butylhydroquinone (tBHQ) (Sigma), 5 μM prostaglandin E2 (pGE2) (Sigma), 1 μM torin (Axon Medchem), 10 μM halofuginone (Santa Cruz Biotechnology), 100 nM bafilomycin (Sigma), 20 μM ZVAD pan-caspase inhibitor (Calbiochem), caspase I inhibitor IV (Calbiochem), 5 μg/mL brefeldin A, 5 μg/mL tunicamycin (Sigma).

O'Connell D.J., Kolde R., Sooknah M., Graham D.B., Sundberg T.B., Latorre I., Mikkelsen T.S, & Xavier R.J. (2016). Simultaneous Pathway Activity Inference and Gene Expression Analysis Using RNA Sequencing. Cell systems, 2(5), 323-334.

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