Lab type sso one lambda typing kit

Genomic DNA from patients and controls was isolated from whole blood samples using QuickGene-800 assays (Fujifilm, Tokyo, Japan). HLA typing in AIH patients was carried out using a Luminex multi-analyzer profiling system with a LAB type SSO One Lambda typing kit (One Lambda, Inc. Canoga Park, CA). HLA genotypes were determined by sequence-based typing, as earlier described^{32 (link)}. The rs9277534 SNP in the HLA-DP locus was genotyped using a TaqMan 5′ exonuclease assay (Applied Biosystems, Tokyo, Japan). The polymerase chain reaction (PCR) was performed with the StepOne Plus Real-Time PCR System (Applied Biosystems) following the manufacturer’s instructions. Pairwise linkage disequilibrium patterns between HLA-DPB1 alleles and rs9277534 (A/G) were assessed by the Arlequin program (ver. 3.5.2.2) (http://lgb.unige.ch/arlequin/)^{33 (link)}. The linkage of rs9277534 to HLA-DPB1 was examined in all AIH patients pursuant to a previous report^{23 (link)}.

Genomic DNA from patients and healthy individuals was isolated by phenolic extraction of sodium dodecyl sulfate-lysed and proteinase K-treated cells, as described previously.[22] (link) HLA typing was carried out using a Luminex multi-analyzer profiling system with a LAB type SSO One Lambda typing kit (One Lambda, Inc. Canoga Park, CA). HLA genotypes were determined by sequence-based typing, as earlier described.[23] (link) HLA-Bw4, HLA-Bw6, HLA-C1, and HLA-C2 KIR ligands were assigned based on the amino acid residues of the HLA-A, HLA-B, and HLA-C alleles. The peptide sequences of all HLA-DRB1, DQB1, and DPB1 alleles in the IMGT/HLA database release 3.14.0 (October 2013) were aligned.