Aspc 1

Manufactured by Keygen Biotech

Sourced in China

The AsPC-1 is a laboratory cell line derived from a human pancreatic adenocarcinoma. It is commonly used in research to study the biology and behavior of pancreatic cancer cells.

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Lab products found in correlation

4 protocols using aspc 1

PANCREATIC CANCER CELL LINES PROTOCOL

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PANC-1, AsPC-1, BxPC-3 and MiaPaca-2 were bought from KeyGEN Biotech (Nanjing, China) and maintained as recommended. The cells cultured in a medium containing different concentrations of DMAPT were grown for 48 h. During this period, all assays, including MTT, cell cycle, Western blotting and IP, were performed every 12 h. All cell lines used in this study were authenticated by short tandem repeat DNA finger-printing and tested for mycoplasma at GENEWIZ, Inc. (Beijing, China) before use. The reports are provided in Supplementary Figure 6.

Shi C., Wang Y., Guo Y., Chen Y, & Liu N. (2017). Cooperative down-regulation of ribosomal protein L10 and NF-κB signaling pathway is responsible for the anti-proliferative effects by DMAPT in pancreatic cancer cells. Oncotarget, 8(21), 35009-35018.

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Diverse Cell Lines for Cancer Research

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The following human cell lines were obtained KeyGen Biotech (Nanjing, China): NCI-H446, and A549 lung cancer cell lines; PLC, SMMC7721, HepG2, MHCC97H, and MHCC97L hepatoma carcinoma lines; LOVO colon cancer line; PC-3 prostate cancer cell line; A875, A375, Mum2B, and Mum2C melanoma lines; MCF-7 and MDA-MB-231 breast cancer cell lines; SGC-7901gastric cancer cell line; PCNA-1 and AsPC-1 pancreatic cancer cell lines; HeLa cervical cancer line; K562 and HL60 leukemia lines; and SH-SY5Y neuroblastoma line. Cells were cultured in medium supplemented with 10% heat-inactivated (56°C, 30 min) fetal calf serum (Hyclone, USA) and maintained at 37°C with 5% CO₂ in a humidified atmosphere. Details are shown in Table S2.

Qin Y., Zhang Q., Lee S., Zhong W.L., Liu Y.R., Liu H.J., Zhao D., Chen S., Xiao T., Meng J., Jing X.S., Wang J., Sun B., Dai T.T., Yang C., Sun T, & Zhou H.G. (2015). Doxycycline reverses epithelial-to-mesenchymal transition and suppresses the proliferation and metastasis of lung cancer cells. Oncotarget, 6(38), 40667-40679.

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Culturing Panc-1 and Aspc-1 Cells

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The human pancreatic cancer cell lines Panc-1 and Aspc-1 were purchased from KeyGEN BioTECH, and maintained in media recommended by the vendors. The human pancreatic cancer cell lines Panc-1 and Aspc-1 were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS). The cells were cultured at 37°C with 5% CO₂ in a humidified atmosphere.

Liu H., Tao H., Wang H., Yang Y., Yang R., Dai X., Ding X., Wu H., Chen S, & Sun T. (2021). Doxycycline Inhibits Cancer Stem Cell-Like Properties via PAR1/FAK/PI3K/AKT Pathway in Pancreatic Cancer. Frontiers in Oncology, 10, 619317.

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Cell Line Culture and Compound Screening

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Aspc-1, HepG2, Panc02, and H22 cell lines were obtained from the KeyGEN Biotechnology Company (China). HT1080 and SW480 were obtained from the FuHeng BioLogy Company (China). HT1080 cancer cells were cultured in Eagle’s Minimum Essential Medium supplemented with 10% fetal bovine serum (FBS), glutamine (2 mM), penicillin (100 U/ml), and streptomycin (0.1 mg/ml). SW480, Aspc-1, HepG2, Panc02, and H22 were cultured in high Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS, L-glutamine (4 mM), and penicillin (100 U/ml) and streptomycin (0.1 mg/ml). All cell lines were maintained in a humidified atmosphere containing 5% CO₂ at 37 °C and tested for mycoplasma prior to the commencement of experiments. Unless otherwise indicated, cell culture medium was changed every 3 days, and cells were passaged using 0.05% trypsin/EDTA. Erastin (#HY-15763), sorafenib (#HY-10201), sulfasalazine (#HY-14655), DON (#HY-108357), RSL3 (#HY-100218A), L-Buthionine-(S,R)-sulfoximine (BSO, #HY-106376A), ferrostatin-1 (#HY-100579), Z-VADFMK (#HY-16658), AICAR (#HY-13417), BafA1 (#HY-100558), N-Acetylcysteine (#HY-B0215), and Necrosulfonamide (#HY-100573) were purchased from MedChemExpress (USA). Compound C (#ab120843) was purchased from Abcam. Deferoxamine mesylate (#D9533) was purchased from Sigma-Aldrich.

Wang K., Zhang Z., Tsai H.I., Liu Y., Gao J., Wang M., Song L., Cao X., Xu Z., Chen H., Gong A., Wang D., Cheng F, & Zhu H. (2020). Branched-chain amino acid aminotransferase 2 regulates ferroptotic cell death in cancer cells. Cell Death and Differentiation, 28(4), 1222-1236.

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