H1819

Lung cancer cell lines (A549, H2009, H1819, Calu3, H23, H1993, H226, H460, and H1299) and the normal bronchial epithelial cell line (BEAS2B) were obtained from the American Type Culture Collection, as previously described [7 (link)]. The cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, Waltham, MA, USA) or RPMI-1640 medium (Thermo Fisher Scientific), supplemented with 1% penicillin–streptomycin and 10% fetal bovine serum.

An immortalized human airway epithelial cell line (16HBE14o, Simian virus 40 (SV40)-transformed human bronchial epithelial cells) described by Cozens AL et al. (1994) [14] (link) was kindly provided by Grunert DC (California Pacific Medical Center Research Institute). A sub-clone of 16HBE14o cells, described as NHBE-T in this study, was used. Immortalized airway epithelial cell lines (HPL1D and HPL1A, SV40-transformed human small airway epithelial cells) were established by Masuda A et al. (1997) [15] (link). Human lung cancer cell lines (A549, H322M, H358, H522, H820, H2087, H23, EKVX, H226, H827, H1819, H441, H4006, HOP62, H1299, and H460) and a human embryonic kidney cell line (HEK293T) were purchased from the American Type Culture Collection (ATCC, Manassas, VA). The human lung cancer cell line LC2AD, Lu130, Lu135, Lu139, and Lu140, was purchased from the Riken Cell Bank (Tsukuba, Japan). The human lung cancer cell lines, PC9 and HARA, were from Immuno-Biological Laboratories Co. (Gunma, Japan). The human lung cancer cell lines, TKB1, TKB2, TKB4, TKB5, TKB6, TKB7, TKB8, TKB9, TKB12, TKB14, TKB15, TBK17, and TKB20, were obtained from Dr. Hiroshi Kamma via Dr. Takuya Yazawa (Kyorin University School of Medicine) [16] (link). Primary small airway epithelial cells (SAEC) and normal human bronchial epithelial cells (NHBE) were purchased from SANKO Kagaku (Tokyo, Japan).