Gardasil

The Danish childhood vaccination programme is voluntary and free of charge. HPV vaccination with a quadrivalent vaccine (Gardasil^®, MSD) was included in the Danish schedule for 12-year-old girls on 1 January 2009, with catch-up vaccination of 13–15 year old girls starting October 2008. The bivalent vaccine (Cervarix^®, GSK) replaced the quadrivalent vaccine between 1 February 2016 and 31 October 2017. Since 1 November 2017, the nonavalent vaccine (Gardasil9^®, MSD) has been used. We obtained information on HPV vaccinations from the Danish Vaccination Registry, which contains individual-level longitudinal information on all vaccinations in the Danish childhood vaccination programme.⁹
We obtained information on birth order, the mother’s age at the time of birth and the mother’s civil status, from the Danish Civil Registration System for each girl in the study cohort.⁸ (link)

The antigen for experimental autoimmune encephalomyelitis (EAE) and pertussis toxin (Ptx) were purchased from Hook Laboratories, and the HPV vaccine, Gardasil, was purchased from MSD K.K. The following antibodies were used in this study: anti- NG2, anti-GAD67 and anti-tyrosine hydroxylase antibodies from Merck Millipore (Germany); anti-GFAP, CD31, anti-choline acetyltransferase and anti-Glutamine synthetase from Abcam (Cambridge, UK); anti-GABA antibody from Sigma-Aldrich.