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Rasputin software

Manufactured by Plexon
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RASPUTIN is a comprehensive data acquisition software suite designed for Plexon's multi-channel neural recording systems. It provides tools for configuring and controlling the hardware, as well as for real-time visualization and analysis of the acquired neural data.

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Lab products found in correlation

Offline sorter, by Plexon (4 mentions) Urethane, by Merck Group (3 mentions) Map recording system, by Plexon (1 mentions)

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RASPUTIN (3 citations)

4 protocols using rasputin software

1

Primate Visual Cortex Electrophysiology

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We recorded multi-unit (MUA) and single-unit (SUA) activity from ventral area V4 (81 MUA, 19 SUA), the thalamic pulvinar (dorsal pulvinar: 102 MUA, 34 SUA; ventral pulvinar: 108 MUA, 12 SUA) and the LGN (89 MUA, 16 SUA) in two adult Rhesus macaques (Macaca mulatta, monkey E and B). Data were recorded during a total of 113 recording sessions (62 monkey E, 51 monkey B) with 4 to 8 microelectrodes simultaneously (Thomas Recording GmbH, Giessen, Germany). Of those sessions, 95 recordings (49 monkey E, 46 monkey B) contained sites that met our inclusion criteria (uninterrupted recordings without drifts, a minimum number of 10 identical trials as well as a minimum mean firing rate of 5 spikes/s) and were considered for analysis. Spiking activity was collected with the MAP recording system (Plexon Inc., Dallas TX, USA). Spike candidates were identified online through RASPUTIN software (Plexon Inc., Dallas TX, USA) on a PC receiving the digitized signals. Waveform thresholds were manually adjusted for each channel before the start of data acquisition. After the experiment, units were isolated in PCA space in the commercially available “Offline Sorter” (Plexon Inc., Dallas TX, USA) and time stamps were saved for further analysis.
Poland E., Donner T.H., Müller K.M., Leopold D.A, & Wilke M. (2019). Thalamus exhibits less sensory variability quenching than cortex. Scientific Reports, 9, 7590.
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2

Single-Unit Recordings in Rat PVT

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Rats infected with eNpHR-eYFP in PL were anesthetized with urethane (1 g/Kg, i.p.; Sigma-Aldrich) and placed in a stereotaxic. An optrode consisting of an optic fiber surrounded by 8 single unit-recording wires (NB Labs) was inserted directed to PVT (−3.1 mm anterior, 1.8 mm lateral, 5.5 mm ventral from bregma with a 20° angle). The optrode was ventrally advanced in steps of 0.03 mm. Single units were monitored online (RASPUTIN software, Plexon Inc). When a single unit was isolated, a 593.5 nm laser was activated for 10 s within a 20 s period, at least 5 times. Single units were recorded and stored for spike sorting (Offline Sorter, Plexon Inc) and spike train analysis (Neuroexplorer, NEX Technologies) as described for single-units recording methods.
Do-Monte F.H., Quinones-Laracuente K, & Quirk G.J. (2015). A temporal shift in the circuits mediating retrieval of fear memory. Nature, 519(7544), 460-463.
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3

Single-Unit Recordings in Rat PVT

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Rats infected with eNpHR-eYFP in PL were anesthetized with urethane (1 g/Kg, i.p.; Sigma-Aldrich) and placed in a stereotaxic. An optrode consisting of an optic fiber surrounded by 8 single unit-recording wires (NB Labs) was inserted directed to PVT (−3.1 mm anterior, 1.8 mm lateral, 5.5 mm ventral from bregma with a 20° angle). The optrode was ventrally advanced in steps of 0.03 mm. Single units were monitored online (RASPUTIN software, Plexon Inc). When a single unit was isolated, a 593.5 nm laser was activated for 10 s within a 20 s period, at least 5 times. Single units were recorded and stored for spike sorting (Offline Sorter, Plexon Inc) and spike train analysis (Neuroexplorer, NEX Technologies) as described for single-units recording methods.
Do-Monte F.H., Quinones-Laracuente K, & Quirk G.J. (2015). A temporal shift in the circuits mediating retrieval of fear memory. Nature, 519(7544), 460-463.
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4

Optogenetic Modulation of Neural Circuits

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Rats expressing eNpHR in aPVT were anaesthetized with urethane (1g/Kg, i.p.; Sigma Aldrich) and mounted in a stereotaxic frame. An optrode consisting of an optical fiber surrounded by 16 single-unit recording wires (NB Labs) was inserted aiming at the NAcsh (AP, +1.0 mm; ML: −0.9; DV: −7.2) or the CeA (AP, −2.8 mm; ML, −4.2 mm; DV, −7.0 mm). The optrode was ventrally advanced in steps of 0.03 mm. Single-units were monitored in real time (RASPUTIN software, Plexon Inc.). After isolating a single-unit, a 593.5 nm laser was activated for 10 s within a 20 s period, at least 5 times. Single-units were recorded and stored for spike sorting (Offline Sorter, Plexon Inc.) and spike-train analysis (Neuorexplorer, NEX Technologies). In NAcsh, a total of 68 neurons were recorded from 3 rats. In CeA, a total of 44 neurons were recorded from 1 rat. Excitatory and inhibitory responses were calculated by comparing the average firing rate of each neuron during the 10 s of laser off with the 10 s of laser on (Paired t-test, 1 s bins).
Do-Monte F.H., Minier-Toribio A., Quiñones-Laracuente K., Medina-Colón E.M, & Quirk G.J. (2017). Thalamic regulation of sucrose-seeking during unexpected reward omission. Neuron, 94(2), 388-400.e4.
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