Bgc803

Five human GC cell lines (AGS, MKN-45, BGC-803, MGC-823, HGC-27) and one human normal gastric mucosal epithelial cell line (GES-1) were purchased from the cell bank of the Chinese Academy of Sciences (Shanghai Cancer Institute, Shanghai, China) or the American Type Culture Collection (Gaithersburg, MD, USA). GC cells and GES-1 cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum (both from Thermo Scientific, Shanghai, China) and 1% penicillin-streptomycin (Beijing Solaibo Technology, Beijing, China) in an incubator at 37°C and 5% CO₂.

Human GC cell lines NCI-N87, SGC7901, BGC823, BGC803, and AGS; human normal gastric epithelium cell line GES-1; human kidney epithelial cell line HEK293; human lung fibroblast CCL-153; and human endothelial cell line EC-304 were all purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). Cells were plated in tissue culture dishes and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; Hyclone, Logan, UT, USA) in a humidified 5% CO₂ incubator (Thermo Fisher Scientific) at 37°C for 2–4 days until 80% confluence was reached. Before experiment treatment, cells were digested using 0.05% trypsin solution (Ameresco, Framingham, MA, USA) and seeded into 6-well or 96-well tissue culture plates.