Recombinant human HMGB1 (rHMGB1) and H-89 was purchased from Sigma (St. Louis, MO). The small hairpin RNA (shRNA) specifically targeting human HMGB1 and their control plasmids were purchased from Open Biosystems (Pittsburgh, PA, USA). Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, CA, USA). 8-Br-cAMP was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Transwell chamber was the product of Corning Company (Corning, NY, USA). Antibodies against CREB, CREB phosphorylation at Ser133, SP1, Cofilin, Profilin, nWASP, nWASP phosphorylation at Tyr256 or Ser484 were products from Cell Signaling Technologies (Beverly, MA). Antibodies against α-Tubulin, GFP, β-Actin, HMGB1, Lamin B, PKA RI, PKA RII, PKA-Cα, and GAPDH were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
8 br camp
Manufactured by Santa Cruz Biotechnology
Sourced in United States
8-Br-cAMP is a chemical compound that serves as a cell-permeable analog of cyclic adenosine monophosphate (cAMP). It functions as a second messenger in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Cofilin, by Cell Signaling Technology (1 mentions)
Pka cα, by Santa Cruz Biotechnology (1 mentions)
N wasp, by Cell Signaling Technology (1 mentions)
Recombinant human hmgb1 rhmgb1, by Merck Group (1 mentions)
Antibodies against α tubulin, by Santa Cruz Biotechnology (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Lamin b, by Santa Cruz Biotechnology (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Transwell chamber, by Corning (1 mentions)
Dulbecco modified eagle medium (dmem), by Fujifilm (1 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using 8 br camp
1
HMGB1 Regulation of Actin Cytoskeleton
2
UV-C Stress Response in Cell Lines
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HCT116 cells were cultured in McCoy's 5A medium (16600‐082; Gibco) containing 10% FBS and antibiotics (15240062; Thermo Fisher Scientific). HEK293T cells were cultured in DMEM (044‐29765; WAKO) containing 10% FBS. All cells were cultured at 37°C under 5% CO2. Cells were treated with 4,5,6,7‐tetrabromobenzotriazole (TBB) (2275; Tocris), H89 (CAY10010556; Cayman Chemical), and 8Br‐cAMP (201564; Santa Cruz). Cells were rinsed with PBS and exposed to 100 J/m2 or 25 J/m2 UV‐C in a StrataLinker 1800 crosslinker (Agilent‐Stratagene, Santa Clara, CA, USA).
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