Fak inhibitor 14

Manufactured by R&D Systems

Sourced in Germany

FAK Inhibitor 14 is a small molecule that inhibits the activity of Focal Adhesion Kinase (FAK). FAK is a non-receptor tyrosine kinase that plays a role in various cellular processes, including cell adhesion, migration, and survival. This product is intended for research use only.

Automatically generated - may contain errors

Lab products found in correlation

Pf 573228, by R&D Systems (1 mentions) α sma, by Agilent Technologies (1 mentions) Phospho fak, by Thermo Fisher Scientific (1 mentions) β actin, by BioLegend (1 mentions) Gapdh, by Merck Group (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions) Ripa lysis buffer, by Santa Cruz Biotechnology (1 mentions)

2 protocols using fak inhibitor 14

Integrin Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following monoclonal antibodies were used: mouse AC-15 to actin (Sigma, München, Germany), mouse B-1 to Akt1 (Santa Cruz, Heidelberg, Germany), rabbit D9E to phospho-Akt (S473) (Cell signaling technology, Danvers, USA), mouse 36/E-cadherin (BD Bioscience, Heidelberg, Germany), mouse 4.47 to phospho-FAK Y397 (Millipore, Darmstadt, Germany) and rat MB1.2 to β1 integrin (Merck, Darmstadt, Germany). The polyclonal antibodies were: rabbit ERK2 (C-14), rabbit GAPDH and rabbit integrin β4 (H-101) (Santa Cruz, Heidelberg, Germany), rabbit phospho-p44/42 MAPK (ERK1/2; T202/Y204) (Cell signaling technology, Danvers, USA), rabbit phospho-FAK (Y397) (Invitrogen, Karlsruhe, Germany), rabbit Keratin 5 (HISS Diagnostic, Freiburg, Germany) and the rabbit Endo-2 antibody to ColXVII [15] (link).
A polyclonal rabbit antibody specific for the phosphorylated residue S1356 on the β4 integrin subunit was kindly provided by A. Sonnenberg (The Netherlands Cancer Institute) [16] .
The inhibitor PI3K (LY294002) was obtained from Merck Biosciences (Darmstadt, Germany). Selective focal adhesion kinase (FAK) inhibitors PF 573228 and FAK Inhibitor 14 were obtained from R&D Systems (Wiesbaden-Nordenstadt, Germany). Recombinant EGF was obtained from ImmunoTools (Friesoythe, Germany). All inhibitors are used in indicated concentrations.

Löffek S., Hurskainen T., Jackow J., Sigloch F.C., Schilling O., Tasanen K., Bruckner-Tuderman L, & Franzke C.W. (2014). Transmembrane Collagen XVII Modulates Integrin Dependent Keratinocyte Migration via PI3K/Rac1 Signaling. PLoS ONE, 9(2), e87263.

+ Open protocol

+ Expand

Renal Protein Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were extracted from renal tissue using RIPA lysis buffer (Santa Cruz Biotechnology). Western blot for CD146 (Epitomics) were performed using standard techniques as previously described. 27 (link) GAPDH (Sigma-Aldrich) was used as loading control.
For siCD146 experiments, glomerular endothelial cells were supplemented or not with 1 µM of focal adhesion kinase (FAK) inhibitor 14 (R&D systems) for 24 hours. Then, cells were lysed in RIPA+PI and protein levels of Vimentin (Cell signaling), α-SMA (Dako), FAK, and phospho-FAK (Life Technologies) were quantified using Western Blot. β-actin (Biolegend) was used as loading control.

Abed A., Leroyer A.S., Kavvadas P., Authier F., Bachelier R., Foucault-Bertaud A., Bardin N., Cohen C.D., Lindenmeyer M.T., Genest M., Joshkon A., Jourde-Chiche N., Burtey S., Blot-Chabaud M., Dignat-George F, & Chadjichristos C.E. (2021). Endothelial-Specific Deletion of CD146 Protects Against Experimental Glomerulonephritis in Mice. Hypertension (Dallas, Tex. : 1979), 77(4).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!