Anti cd140a

Manufactured by BioLegend

Sourced in United States

Anti-CD140a is a monoclonal antibody that binds to the CD140a (also known as PDGFRα) cell surface receptor. CD140a is a member of the platelet-derived growth factor receptor family and is expressed on various cell types, including mesenchymal stem cells, fibroblasts, and certain tumor cells. The core function of Anti-CD140a is to facilitate the detection and analysis of cells expressing CD140a.

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Lab products found in correlation

Anti cd31, by BioLegend (3 mentions) Anti cd45, by BioLegend (2 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) Anti cd45, by Thermo Fisher Scientific (1 mentions) Anti ki67, by Thermo Fisher Scientific (1 mentions) Anti ly 6a e sca 1, by BioLegend (1 mentions) Anti mouse cd16 cd32 fc block clone 2.4g2, by BD (1 mentions) Aria 3 flow cytometer, by BD (1 mentions) Anti cd26, by BioLegend (1 mentions) Anti ter119, by BioLegend (1 mentions)

Spelling variants of this product

CD140a (12 citations) Anti‐PDGFRa (CD140a)‐PE (2 citations) PE-conjugated anti-PDGFRA (CD140a) antibody (2 citations) Rat anti‐CD140a (2 citations) PE anti-mouse CD140a (PDGFRa) (2 citations) Anti-PDGFRα (CD140a)-APC (2 citations) PE-conjugated anti-CD140a (2 citations)

3 protocols using anti cd140a

Isolation and Analysis of Stromal Vascular Cells

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Stromal Vascular cells (SVC) from the donor site after mechanical stress were isolated as previously described [65 (link)]. Briefly, adipose tissues were digested in phosphate-buffered saline (PBS) with 0.5% BSA and 1 mg/mL type II collagenase for 25 min at 37 °C, and stromal vascular cells (SVC) were separated from adipocytes by centrifugation. Isolated SVC were stained using live/dead fixable dyes (Invitrogen): anti-CD45 (eBioscience, 30-F11), anti-Ly-6A/E(Sca1) (BioLegend, D7, San Diego, CA, USA), anti-CD31 (BioLegend, 390, San Diego, CA, USA), and anti-CD140a (BioLegend, APA5, San Diego, CA, USA) and anti-Ki67 (eBioscience, SolA15, San Diego, CA, USA). Cells were analyzed on a FACS Canto II Flow Cytometer (BD Biosciences, San Jose, CA, USA) using the FlowJo 10.6 software (Tree Star, Ashland, OR, USA).

Chun J.J., Chang J., Soedono S., Oh J., Kim Y.J., Wee S.Y., Cho K.W, & Choi C.Y. (2022). Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation. International Journal of Molecular Sciences, 23(19), 11839.

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Isolation and Analysis of Adipose-Derived SVF Cells

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Samples of SVF cells from the mammary glands (subcutaneous adipose tissue) were first incubated on ice for 20 minutes in 200 uL of 2% FBS/PBS containing anti-mouse CD16/CD32 Fc Block (clone 2.4G2, BD) (1:200). Cells were then incubated with primary antibody (anti-CD31, 1:200, anti-CD45, 1:200, anti-CD140a, 1:100, all from BioLegend) and were incubated rotating at 4°C for 30 minutes. They were then washed three times with 2% FBS/PBS, and either analyzed using a FACSCantoIITM flow cytometer or sorted by a FACSAriaTM flow cytometer (UT Southwestern Medical Center Flow Cytometry Core Facility). For sorting, cells were initially selected by size, on the basis of forward scatter (FSC) and side scatter (SSC). Live cells were then gated on both SSC and FSC Width singlets, ensuring that individual cells were analyzed. SVF cells isolated from wild-type mice, along with fluorescent-minus-one (FMO) controls, were used to determine background fluorescence levels. Cells were sorted into FBS and then cultured in a 24-well dish. All flow cytometry antibodies were rat-derived and conjugated to APC or PE.

Wang Q.A., Song A., Chen W., Schwalie P.C., Zhang F., Vishvanath L., Jiang L., Ye R., Shao M., Tao C., Gupta R.K., Deplancke B, & Scherer P.E. (2018). Reversible De-differentiation of Mature White Adipocytes into Preadipocyte-like Precursors During Lactation. Cell metabolism, 28(2), 282-288.e3.

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Fibroblast Immunophenotyping by Flow Cytometry

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After the extraction of fibroblasts, they were incubated with antibodies and left to sit at 4 °C for 30 min. Subsequently, the antibodies were washed off, and the cells were resuspended and sorted using a BD Aria III flow cytometer. The antibodies used for staining were as follows: anti-CD31 (BioLegend #102417), anti-ter119 (BioLegend #116221), anti-CD45 (BioLegend #100432), anti-CD140a (BioLegend #135907), anti-CD142 (Sino Biological #50413-RP01), anti-CD26 (BioLegend #137804), and anti-CD36 (ThermoFisher #56-0362-82). FITC coupling was required for the anti-CD26 antibody, and both antibodies were used at a 1:100 dilution.

Li J., Ma R., Wang X., Lu Y., Chen J., Feng D., Zhou J., Xia K., Klein O., Xie H, & Lu P. (2024). Sprouty genes regulate activated fibroblasts in mammary epithelial development and breast cancer. Cell Death & Disease, 15(4), 256.

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