Novex tbe urea gel
The Novex TBE-Urea gel is a pre-cast polyacrylamide gel designed for high-resolution separation of nucleic acid samples under denaturing conditions. The gel is formulated with a Tris-Borate-EDTA (TBE) buffer and contains urea to maintain the denaturing environment, enabling the analysis of single-stranded DNA and RNA samples.
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42 protocols using novex tbe urea gel
Small RNA-Seq Library Preparation and Analysis
RNA Extraction and Small RNA Sequencing
Small RNAs (20–30 nt) were purified from 15% Novex TBE-Urea Gel (Invitrogen) and ligated first with the 5’ RNA adaptor and then with the 3’ RNA adaptor provided by Illumina TruSeq small RNA sample preparation protocol. In each step, the ligated product was PAGE-gel purified. After first-strand synthesis and 11 cycles of PCR amplification, the product was PAGE-gel purified and submitted for sequencing on an Illumina NextSeq500 at LAS (Seoul, Korea;
Quantifying Steady-State mt-tRNA Levels
Northern Blot for RNA Analysis
For Northern blot: After UV crosslinking, the membrane was blocked by ExpressHyb Hybridization Solution (Takara Bio #636833) and probed with biotinylated DNA probe (probe sequences see Supplementary Table
For immuno-Northern blot: After UV crosslinking, the membrane was blocked with 3% milk in PBST and detected by m1A primary antibody (MBL #D3453, used at 1:2000 dilution) followed by anti-mouse HRP-linked secondary antibody (Cell Signaling #7076, used at 1:5000 dilution). Chemiluminescence detection was performed with Immobilon HRP substrate (Millipore #WBKLS0500). Oligo size was compared to microRNA marker (NEB #N2102S) on the gel.
Ring-nuclease-mediated cOA4 processing
Bacterial RNA Extraction and Purification
Northern Blot Analysis of Mvh Expression
Northern Blot Analysis of RNA
Cas12a Family-Mediated DNA Cleavage
TracrRNA Stability Determination in FBS
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