1290 infinity 2 lc 6545 quadrupole tof

Manufactured by Agilent Technologies

Sourced in United States

The 1290 Infinity II LC-6545 Quadrupole-TOF is a liquid chromatography-mass spectrometry (LC-MS) system designed for high-performance analytical applications. It combines a high-performance liquid chromatography (HPLC) system with a quadrupole time-of-flight (Q-TOF) mass spectrometer to provide accurate mass measurement and identification of compounds.

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Lab products found in correlation

Eclipse plus c18 rapid resolution hd column, by Agilent Technologies (2 mentions) Eclipse plus c18, by Agilent Technologies (1 mentions) Zorbax eclipse plus c18 column, by Agilent Technologies (1 mentions) Zorbax eclipse plus column, by Agilent Technologies (1 mentions)

Close spelling variants of this product

1290 Infinity II LC 1290 Infinity LC 1290 Infinity II 1290 Infinity Infinity II

5 protocols using 1290 infinity 2 lc 6545 quadrupole tof

Quantitative Analysis of Eucam in Methanol-Water

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Briefly
Lipo-Eucam was measured and dissolved in methanol: water (70:30 v/v).
The solution was thoroughly vortexed and centrifuged (8000 rpm for
10 min). Thereafter, the Eucam solution was filtered through a 0.2
μm nylon membrane syringe filter. The clear Eucam solution was
then analyzed by UPLC-DAD-ESI-QTOF-MS as previously described^{59 (link)} using liquid chromatograph-quadrupole time-of-flight
mass spectrometer (LC-QTOF MS), 1290 Infinity II LC-6545 Quadrupole-TOF
(Agilent Technologies, USA) with a Zorbax Eclipse Plus C18 2.1 ×
150 mm, 1.8 μm column.

Huang Y., An M., Fang A., Olatunji O.J, & Eze F.N. (2022). Antiproliferative Activities of the Lipophilic Fraction of Eucalyptus camaldulensis against MCF-7 Breast Cancer Cells, UPLC-ESI-QTOF-MS Metabolite Profile, and Antioxidative Functions. ACS Omega, 7(31), 27369-27381.

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Ethanol-Soluble Lipid Extraction and Analysis

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Ethanol soluble lipids were prepared using the method of Raju et al. (18 (link)). Briefly, oil samples (100 mg) were added to 1 ml of ethanol and vortexed vigorously. The prepared mixture was then stored at −20°C for 3 h. At the end of the incubation period, the mixtures were centrifuged at 4,000 × g and the upper phase was collected. The upper phase (500 μl) was subjected to liquid chromatography quadrupole time-of-flight mass spectrometer (LC-QTOF MS), 1290 Infinity II LC-6545 Quadrupole-TOF (Agilent Santa Clara, CA, USA). The instrument was equipped with Zorbax Eclipse Plus C18 column (150 mm length × 2.1 mm inner-diameter, particle size 1.8 μm) (Agilent Santa Clara, CA, USA). Mobile phase A: 50 mM ammonium acetate/methanol [20/80 (v/v)] and B: Isopropanol/methanol [70/30 (v/v)] were used for the separation. Positive atmospheric pressure chemical ionization was done and compounds were identified based on mass and spectrum compared with MassHunter METLIN PCD library.

Raju N., Gulzar S., Buamard N., Ma L., Ying X., Zhang B, & Benjakul S. (2021). Comparative Study of Astaxanthin, Cholesterol, Fatty Acid Profiles, and Quality Indices Between Shrimp Oil Extracted From Hepatopancreas and Cephalothorax. Frontiers in Nutrition, 8, 803664.

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LC-QTOF MS Analysis of M. oleifera Leaf Extract

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The extract of M. oleifera leaf (100 mg/mL) in 50% MeOH was ultra-sonicated for 20 min and centrifuged (10,000 rpm at 4 °C) for 10 min. The supernatant was filtrated through the 0.2 µm nylon membrane and the filtrate was injected into a liquid chromatograph-quadrupole time-of-flight mass spectrometer (LC-QTOF MS), (1290 Infinity II LC-6545 Quadrupole-TOF, Agilent Technologies, Santa Clara, CA, USA). HPLC was equipped with a Zorbax Eclipse Plus column (C18 2.1 × 150 mm, 1.8 µ, Agilent Technologies, Santa Clara, CA, USA). Formic acid (0.1%) in water (solvent A) and acetonitrile (solvent B) were used as the mobile phase and the gradient elution was as follows: 98% A, 0–2 min; 90% A, 2–25 min; 85% A, 25–40 min; 80% A, 40–48 min; 75% A, 48-68 min; 70% A, 68–80 min; 50% A, 80–85 min; 0% A, 85–90 min.; 98% A, 90–100 min. Peaks were detected at the wavelengths of 254 nm and 280 nm. The MS spectra were acquired in both positive and negative ion modes with auto MS/MS. Full-scan mode from m/z 100 to 1700 was applied. The HPLC peaks were identified by using the spectrum database for organic compounds in the METLIN database.

Hashim F.J., Vichitphan S., Han J, & Vichitphan K. (2021). Alternative Approach for Specific Tyrosinase Inhibitor Screening: Uncompetitive Inhibition of Tyrosinase by Moringa oleifera. Molecules, 26(15), 4576.

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Metabolite Profiling of Medicinal Extracts

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The metabolite profiles of M. fragrans extract, A. lancea extract, and Prabchompoothaweep remedy extract were determined using by an ultra-high performance liquid chromatography (UHPLC) instrument equipped with an electrospray ionization source (ESI). The UHPLC system consisted of a Zorbax Eclipse Plus C18 Rapid Resolution HD column (150 mm length × 2.1 mm inner-diameter, particle size 1.8 µm) with an LC-QTOF MS instrument (1290 Infinity II LC-6545 Quadrupole-TOF, Agilent Technologies, Santa Clara, CA, USA). The mobile phase comprised solvent A (0.1% formic acid in water) and solvent B (acetonitrile). The volume of injection was 2.0 µL, and the column temperature was set at 25 °C. Qualitative analysis of LC-MS/MS was performed in negative ion mode with a scanning range from m/z 100 to 1200 using a Dual AJS ESI ion source. The phytochemical compounds in the extract samples were identified by comparing the retention time, mass data, and fragmentation patterns with known compounds in the library search of the Mass Hunter METLIN database (Agilent Technologies). The compound selection was selected and identified from the peak with 90% similarity in the database.

Plirat W., Chaniad P., Phuwajaroanpong A., Septama A.W, & Punsawad C. (2022). Phytochemical, Antimalarial, and Acute Oral Toxicity Properties of Selected Crude Extracts of Prabchompoothaweep Remedy in Plasmodium berghei-Infected Mice. Tropical Medicine and Infectious Disease, 7(12), 395.

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Tri-TT Extract Composition Analysis

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The composition of Tri-TT extract was analyzed by UHPLC with a column from Zorbax Eclipse Plus C18 Rapid Resolution HD column (150 mm length^∗ 2.1 mm inner diameter, particle size 1.8 μm), using a liquid chromatograph-quadrupole time-of-flight mass spectrometry (LC-QTOF MS) instrument (1290 Infinity II LC-6545 Quadrupole-TOF, Agilent Technologies, USA). The temperature was maintained at 40°C, and the injection volume was 2 μL. Elution was performed with the following 30 min, and mobile phase program was as follows: A: 0.1% formic acid in water, B: acetonitrile, and flow rate: 0.2 mL/min. LC–MS/MS analysis was performed in negative ion mode with a scanning range from m/z 100 to 1500 using a Dual AJS ESI ion source.

Wetchakul P., Chonsut P., Punsawad C, & Sanpinit S. (2022). LC-QTOF-MS Characterization, Antioxidant Activity, and In Vitro Toxicity of Medicinal Plants from the Tri-Than-Thip Remedy. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 4477003.

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