B16 f10 luc

Manufactured by American Type Culture Collection

Sourced in United States

The B16–F10-luc is a cell line that expresses the firefly luciferase reporter gene. It is derived from the B16-F10 mouse melanoma cell line. The core function of this product is to serve as a tool for researchers to study various biological processes, such as gene expression, signal transduction, and tumor growth, in a standardized and reproducible manner.

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Lab products found in correlation

Hemn lp, by Thermo Fisher Scientific (1 mentions) Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) B16f10 luc, by PerkinElmer (1 mentions) 4t1 luc, by PerkinElmer (1 mentions) 4t1 luc, by American Type Culture Collection (1 mentions) Rpmi 1640 medium, by Macgene (1 mentions) Penicillin, by Macgene (1 mentions) Streptomycin, by Macgene (1 mentions) Dulbecco s modified eagle s medium, by Macgene (1 mentions) Fetal bovine serum (fbs), by Macgene (1 mentions)

4 protocols using b16 f10 luc

Melanoma Cell Culture Protocols

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B16–F10-luc murine melanoma cell line was obtained from the ATCC and cultured in RPMI 1640 supplemented with 10% FBS, 100 IU/mL Penicillin, and 100 μg/mL Streptomycin. The human melanocyte cell line HEMn-LP was obtained from Invitrogen and grow in Medium 254 supplemented with HMGS. Human melanoma cell lines were obtained from Dr. Smalley’s Lab at Moffitt Cancer Center. All cell lines were grown under humidified conditions at 37 °C and 5% CO₂.

Woan K.V., Lienlaf M., Perez-Villaroel P., Lee C., Cheng F., Knox T., Woods D.M., Barrios K., Powers J., Sahakian E., Wang H.W., Canales J., Marante D., Smalley K.S., Bergman J., Seto E., Kozikowski A., Pinilla-Ibarz J., Sarnaik A., Celis E., Weber J., Sotomayor E.M, & Villagra A. (2015). Targeting histone deacetylase 6 mediates a dual anti‐melanoma effect: Enhanced antitumor immunity and impaired cell proliferation. Molecular Oncology, 9(7), 1447-1457.

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Culturing Murine and Human Melanoma Cell Lines

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B16-F10-luc murine melanoma cell line was obtained from ATCC and cultured in RPMI 1640 supplemented with 10% FBS, 100IU/mL Penicillin, and 100μg/mL Streptomycin. SM1 cell line was obtained from Dr. Antoni Ribas's Lab at University of California Los Angeles. Human melanoma cell lines were obtained from Dr. Smalley's Lab at Moffitt Cancer Center. Cells were cultured in RPMI 1640 media, supplemented with 10% FBS, penicillin/streptomycin (50 U/ml), L-glutamine (2 mM), and 2-mercaptoethanol (50μM) (complete media), and grown under humidified conditions at 37 °C and 5% CO₂.

Lienlaf M., Perez-Villarroel P., Knox T., Pabon M., Sahakian E., Powers J., Woan K.V., Lee C., Cheng F., Deng S., Smalley K.S., Montecino M., Kozikowski A., Pinilla-Ibarz J., Sarnaik A., Seto E., Weber J., Sotomayor E.M, & Villagra A. (2016). Essential role of HDAC6 in the regulation of PD‐L1 in melanoma. Molecular Oncology, 10(5), 735-750.

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Culturing Mouse Melanoma and Mammary Carcinoma Cells

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The mouse melanoma cell line B16F10-Luc and the mouse mammary carcinoma cell line 4T1-Luc were obtained from the American Type Culture Collection. B16F10-Luc and 4T1-Luc cells were obtained from PerkinElmer. The culture medium for B16F10-Luc cells was Dulbecco’s modified Eagle’s medium (HyClone) containing 10% fetal bovine serum (Invitrogen) and penicillin and streptomycin (100 U ml⁻¹) (Invitrogen). The culture medium for 4T1-Luc cells was RPMI 1640 medium (HyClone) containing 10% fetal bovine serum (Invitrogen) and penicillin and streptomycin (100 U ml⁻¹) (Invitrogen). Cells were tested every 3 months for potential mycoplasma. Re-authentication of cells was not performed after receipt.

Han X., Shen S., Fan Q., Chen G., Archibong E., Dotti G., Liu Z., Gu Z, & Wang C. (2019). Red blood cell–derived nanoerythrosome for antigen delivery with enhanced cancer immunotherapy. Science Advances, 5(10), eaaw6870.

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Cell Culture Conditions for Cancer and Immune Cell Lines

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Human embryonic kidney 293T (HEK 293T) cells were purchased from the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (Beijing, China). Mouse dendritic (DC 2.4) cells were purchased from BeNa Culture Collection (Xinyang, Henan, China). Mouse melanoma cell lines (B16F10, B16F10-Luc, B16F10-OVA) and breast cancer 4T1 cells were purchased from American Type Culture Collection (ATCC, USA). HEK 293T cells, melanoma cells and DC 2.4 cells were cultured in Dulbecco's modified Eagle's medium (Macgene, Beijing, China) at 37 °C under 5 % CO₂. 4T1 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Macgene, Beijing, China) at 37 °C under 5 % CO₂. All these culture media were supplemented with 10 % fetal bovine serum (FBS; PAN, Beijing local agent, Germany), 100 U/mL penicillin, and 100 μg/mL streptomycin (Macgene, Beijing, China).

Li P., Xie Y., Wang J., Bao C., Duan J., Liu Y., Luo Q., Xu J., Ren Y., Jiang M., Li J., Guo H., Zhao H., Wang G., Liang Y, & Lu W. (2024). Gene engineered exosome reverses T cell exhaustion in cancer immunotherapy. Bioactive Materials, 34, 466-481.

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