Ccr2rfp rfp b6.129 cg ccr2tm2 1ifc j

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This mouse strain is a homozygous knock-in of the red fluorescent protein (RFP) reporter gene into the C-C chemokine receptor type 2 (Ccr2) locus. It can be used to track Ccr2-expressing cells in vivo.

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Lab products found in correlation

Cx3cr1 gfp, by Jackson ImmunoResearch (1 mentions) C57bl 6jrj male mice, by Janvier Labs (1 mentions) C57bl 6j b6, by Jackson ImmunoResearch (1 mentions) B6.129p cx3cr1tm1litt j, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

B6.129(Cg)-Ccr2tm2.1Ifc/J (12 citations) Ccr2RFP (7 citations) CCR2RFP/RFP (6 citations)

4 protocols using ccr2rfp rfp b6.129 cg ccr2tm2 1ifc j

Genetically Modified Mice for Immunology

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Wild-type (WT) C57BL/6 mice were purchased from the National Cancer Institute (Frederick, MD, USA). Homozygous CCL2 deficient (CCL2^−/−) mice were originally obtained from B. J. Rollins (Dana-Farber Cancer Institute, Boston, MA, USA). CX3CR1^GFP/GFP (B6.129P-Cx3cr1^tm1Litt/J) and CCR2^RFP/RFP (B6.129(Cg)-Ccr2^tm2.1Ifc/J) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA) and crossed to generate CX3CR1^GFP/+CCR2^RFP/+ mice. Transgenic mice were bred and maintained at the Biological Research Institute under sterile conditions. All procedures were preformed in compliance with the Cleveland Clinic Institutional Animal Care and Use Committee approved protocols.

Savarin C., Dutta R, & Bergmann C.C. (2018). Distinct Gene Profiles of Bone Marrow-Derived Macrophages and Microglia During Neurotropic Coronavirus-Induced Demyelination. Frontiers in Immunology, 9, 1325.

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C57BL/6JRj and Ccr2 Mice Protocol

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Eight to 12-weeks-old C57BL/6JRj male mice were purchased from Janvier SA (Le Genest-Saint-Isle, France). Ccr2^RFP/RFP (B6.129(Cg)-Ccr2^tm2.1Ifc/J) mice were purchased from Jackson Laboratory (Bar Harbor, USA). Mice were maintained at the Institut de la Vision animal facility under pathogen-free conditions. All animals were housed in a 12-h/12-h light/dark cycle with food and water available ad libitum.

Roubeix C., Dominguez E., Raoul W., Guillonneau X., Paques M., Sahel J.A, & Sennlaub F. (2019). Mo-derived perivascular macrophage recruitment protects against endothelial cell death in retinal vein occlusion. Journal of Neuroinflammation, 16, 157.

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Transgenic Mouse Models for Immune Tracking

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C57BL/6J (B6), B6.129P-CX3CR1^tm1Litt/J (Cx3cr1^gfp/gfp), and B6.129(Cg)-CCR2^tm2.1Ifc/J (Ccr2^rfp/rfp) mice were purchased from Jackson Laboratories. Cx3cr1^gfp/+Ccr2^rfp/+ double reporter mice were generated by breeding Cx3cr1^gfp/gfp and Ccr2^rfp/rfp together. Lysm^gfp/+ were provided by T. Graf^{56 (link)} and maintained in closed breeding colony at the NIH. All mice in this study were handled in accordance with the guidelines set forth by the NIH Animal Care and Use Committee.

Russo M.V., Latour L.L, & McGavern D.B. (2018). Distinct myeloid cell subsets promote meningeal remodeling and vascular repair after mild traumatic brain injury. Nature immunology, 19(5), 442-452.

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Intravital Imaging and Monocyte Profiling

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Male 8- to 10-week-old wild-type C57BL/6 J mice, weighing approximately 25 g, were used for all experiments, unless otherwise stated. All mice were purchased from BioLASCO or National Laboratory Animal Center, Taiwan. Mice were housed in a 12-hour day/night cycle with unlimited access to food and water. Homozygous B6.129(Cg)-Ccr2^tm2.1Ifc/J (CCR2^RFP/RFP) and B6.129P2(Cg)-Cx3cr1^tm1Litt/J (CX3CR1^GFP/GFP) mice were purchased from the Jackson laboratory, USA. Heterozygous CCR2^RFP/+ and CX3CR1^GFP/+ mice were generated from Institute of Biomedical Sciences, Academia Sinica, Taiwan. For both intravital imaging and monocyte profiling, 6- to 8-week-old CCR2^RFP/+ mice were used, while 10- to 12-week-old CX3CR1^GFP/+ mice were used for intravital imaging. All mouse experiments have been approved by Academia Sinica Institutional Animal Care and Use Committee.

Huang S.S., Lee K.J., Chen H.C., Prajnamitra R.P., Hsu C.H., Jian C.B., Yu X.E., Chueh D.Y., Kuo C.W., Chiang T.C., Choong O.K., Huang S.C., Beh C.Y., Chen L.L., Lai J.J., Chen P., Kamp T.J., Tien Y.W., Lee H.M, & Hsieh P.C. (2021). Immune cell shuttle for precise delivery of nanotherapeutics for heart disease and cancer. Science Advances, 7(17), eabf2400.

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