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Cenp a and anti ssdna

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CENP-A is a recombinant protein that serves as a marker for centromeres. Anti-ssDNA is an antibody that binds to single-stranded DNA. These lab equipment products are used in research applications, but their core functions are to provide tools for the study of cellular processes and molecular biology without interpretation of intended use.

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Lab products found in correlation

Dld 1 cells, by Merck Group (2 mentions) Flag m2 3165, by Merck Group (2 mentions) Rat anti brdu bu1 75 and fancd2, by Novus Biologicals (2 mentions) Hela cell, by American Type Culture Collection (2 mentions) Bromodeoxyuridine (brdu), by Merck Group (2 mentions) Bromodeoxyuridine (brdu), by BD (2 mentions) β actin antibody, by Santa Cruz Biotechnology (2 mentions)

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Anti–CENP-A (2 citations)

2 protocols using cenp a and anti ssdna

1

PTEN Regulation of DNA Damage Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pten+/+ and Pten−/− mouse embryo fibroblasts (MEFs, provided by P.P. Pandolfi, Harvard Medical School), PTEN+/+ and PTEN−/− HCT116 cells (a kind gift from T. Waldman, Georgetown University), PTEN+/+ and PTEN−/− DLD-1 cells (from Sigma-Aldrich) and HeLa cells (from ATCC) were cultured in MEM supplemented with 10% FBS. The pSUPER RNAi system (Oligoengine Inc.) was used to construct PTEN-specific shRNA expression plasmids and scrambled control shRNA. PTEN, Rad51 and Chk1 expression plasmids were constructed in a pCMV expression vector. BrdU, IdU (5-Iodo-2′-deoxyuridine), CldU (5-Chloro-2′-deoxyuridine), APH (aphidicolin) and HU (hydroxyurea) were from Sigma-Aldrich. The following antibodies were used: PTEN (A2B1), Rad51 (H92), PCNA (FL-261), Chk1 (G4), PARP1/2 (H250), β-Actin antibodies from Santa Cruz; BrdU from BD Pharmingen; CENP-A and anti-ssDNA from EMD Millipore; Rat anti-BrdU BU1/75 and FANCD2 from Novus; FLAG (M2-3165) from Sigma-Aldrich.
He J., Kang X., Yin Y., Chao K.S, & Shen W.H. (2015). PTEN Regulates DNA Replication Progression and Stalled Fork Recovery. Nature communications, 6, 7620.
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2

PTEN Regulation of DNA Damage Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pten+/+ and Pten−/− mouse embryo fibroblasts (MEFs, provided by P.P. Pandolfi, Harvard Medical School), PTEN+/+ and PTEN−/− HCT116 cells (a kind gift from T. Waldman, Georgetown University), PTEN+/+ and PTEN−/− DLD-1 cells (from Sigma-Aldrich) and HeLa cells (from ATCC) were cultured in MEM supplemented with 10% FBS. The pSUPER RNAi system (Oligoengine Inc.) was used to construct PTEN-specific shRNA expression plasmids and scrambled control shRNA. PTEN, Rad51 and Chk1 expression plasmids were constructed in a pCMV expression vector. BrdU, IdU (5-Iodo-2′-deoxyuridine), CldU (5-Chloro-2′-deoxyuridine), APH (aphidicolin) and HU (hydroxyurea) were from Sigma-Aldrich. The following antibodies were used: PTEN (A2B1), Rad51 (H92), PCNA (FL-261), Chk1 (G4), PARP1/2 (H250), β-Actin antibodies from Santa Cruz; BrdU from BD Pharmingen; CENP-A and anti-ssDNA from EMD Millipore; Rat anti-BrdU BU1/75 and FANCD2 from Novus; FLAG (M2-3165) from Sigma-Aldrich.
He J., Kang X., Yin Y., Chao K.S, & Shen W.H. (2015). PTEN Regulates DNA Replication Progression and Stalled Fork Recovery. Nature communications, 6, 7620.
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