Elf1 cells, a naïve human ESC line isolated from an 8-cell human embryo, were procured from Wicell (Madison, WI, USA) and maintained according to a previously published protocol [22 (link)]. Briefly, Elf1 cells were grown adherent to mouse embryonic feeder (MEF) coated 6-well plates and incubated in a medium containing KnockOut/F12 DMEM with GlutaMax (Life Technologies, Carlsbad, CA, USA) with 20% KnockOut serum replacement (Life Technologies), 1 mM sodium pyruvate (Life Technologies), 0.1 mM 2-Mercaptoehtanol (Life Technologies), 1% non-essential amino acids (Life Technologies), and 0.2% penicillin-streptomycin solution (Life Technologies), and supplemented with 12 ng/mL FGF2 (Prospec, Ness Ziona, Israel), 1.5 μM CHIR99021 (Cayman Chemical, Ann Arbor, MI, USA), 0.4 μM PD03296501 (Caymen Chemical) and 0.01 μg/mL human LIF (Prospec). Prior to encapsulation in 3-D scaffolds, cells were transitioned to feeder-free culture in Matrigel-coated 6-well plates (Fisher Scientific, Pittsburgh, PA, USA).
Knockout f12 dmem with glutamax
Manufactured by Thermo Fisher Scientific
Sourced in United States
KnockOut/F12 DMEM with GlutaMax is a cell culture medium designed for the growth and maintenance of a variety of cell types, including embryonic stem cells and other pluripotent cells. It contains a combination of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 nutrient mixture, as well as GlutaMax, a stable glutamine alternative.
Automatically generated - may contain errors
Lab products found in correlation
Sodium pyruvate, by Thermo Fisher Scientific (2 mentions)
Knockout serum replacement (ksr), by Thermo Fisher Scientific (2 mentions)
2 mercaptoehtanol, by Thermo Fisher Scientific (2 mentions)
Human lif, by ProSpec (2 mentions)
Matrigel coated 6 well plates, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (2 mentions)
Chir99021, by Cayman Chemical (2 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions)
Fibroblast growth factor 2 (fgf2), by ProSpec (2 mentions)
2 protocols using knockout f12 dmem with glutamax
1
Naive Human ESC Line Maintenance
2
Cultivating Naïve Human ESCs on MEFs
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Elf1 cells, a naïve human ESC line isolated from an 8-cell human embryo, were procured from Wicell (Madison, WI), and maintained according to a previously published protocol [22] . Briefly, Elf1 cells were grown adherent to mouse embryonic feeder (MEF) coated 6-well plates, and incubated in medium containing KnockOut/F12 DMEM with GlutaMax (Life Technologies, Carlsbad, CA) with 20% KnockOut serum replacement (Life Technologies), 1mM sodium pyruvate (Life Technologies), 0.1mM 2-Mercaptoehtanol (Life Technologies), 1% non-essential amino acids (Life Technologies), and 0.2% penicillin-streptomycin solution (Life Technologies), and supplemented with 12ng/ml FGF2 (Prospec, Ness Ziona, Israel), 1.5µM CHIR99021 (Cayman Chemical, Ann Arbor, MI), 0.4µM PD03296501 (Caymen Chemical) and 0.01µg/ml human LIF (Prospec). Prior to encapsulation in 3-D scaffolds, cells were transitioned to feeder-free culture in Matrigel-coated 6-well plates (Fisher Scientific, Pittsburgh, PA).
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