Log In Sign up
The largest database of trusted experimental protocols

Gentra puregene tissue kit 4g

Manufactured by Qiagen
Visit Supplier
Sourced in Germany

The Gentra Puregene tissue kit 4g is a laboratory equipment product designed for the purification of genomic DNA from a variety of tissue samples. It provides a simple and efficient method for extracting high-quality DNA from various tissue types.

Automatically generated - may contain errors

Lab products found in correlation

Nanodrop, by Thermo Fisher Scientific (2 mentions) Quant it picogreen dsdna assay, by Thermo Fisher Scientific (2 mentions) Tapestation system, by Agilent Technologies (1 mentions) Genomic dna screentape, by Agilent Technologies (1 mentions) Hiseq 2000, by Illumina (1 mentions) Nextera dna kit, by Illumina (1 mentions)

Spelling variants of this product

Gentra Puregene kit (329 citations) Gentra Puregene Tissue Kit (233 citations) Puregene kit (161 citations) Gentra Puregene (27 citations) Puregene Tissue Kit (16 citations)

4 protocols using gentra puregene tissue kit 4g

1

Comprehensive DNA Extraction and Quality Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples included were extracted using the Gentra Puregene tissue kit 4g (Qiagen, Hilden, Germany), following the manufacturer's instructions. All DNA samples were quantified by the fluorometric method (Quant-iT PicoGreen dsDNA Assay, Life Technologies, CA, USA), and assessed for purity by NanoDrop (Thermo Scientific, MA, USA) 260/280 and 260/230 ratio measurements. DNA integrity of Fresh Frozen samples was checked by electrophoresis in a 1.3% agarose gel.
Alcala N., Leblay N., Gabriel A.A., Mangiante L., Hervas D., Giffon T., Sertier A.S., Ferrari A., Derks J., Ghantous A., Delhomme T.M., Chabrier A., Cuenin C., Abedi-Ardekani B., Boland A., Olaso R., Meyer V., Altmuller J., Le Calvez-Kelm F., Durand G., Voegele C., Boyault S., Moonen L., Lemaitre N., Lorimier P., Toffart A.C., Soltermann A., Clement J.H., Saenger J., Field J.K., Brevet M., Blanc-Fournier C., Galateau-Salle F., Le Stang N., Russell P.A., Wright G., Sozzi G., Pastorino U., Lacomme S., Vignaud J.M., Hofman V., Hofman P., Brustugun O.T., Lund-Iversen M., Thomas de Montpreville V., Muscarella L.A., Graziano P., Popper H., Stojsic J., Deleuze J.F., Herceg Z., Viari A., Nuernberg P., Pelosi G., Dingemans A.M., Milione M., Roz L., Brcic L., Volante M., Papotti M.G., Caux C., Sandoval J., Hernandez-Vargas H., Brambilla E., Speel E.J., Girard N., Lantuejoul S., McKay J.D., Foll M, & Fernandez-Cuesta L. (2019). Integrative and comparative genomic analyses identify clinically relevant pulmonary carcinoid groups and unveil the supra-carcinoids. Nature Communications, 10, 3407.
+ Open protocol
+ Expand
2

Efficient DNA Extraction and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Included samples were extracted using the Gentra Puregene Tissue Kit (4 g) (158667; Qiagen), following the manufacturer’s instructions. All DNA samples were quantified using the fluorometric method (Quant-iT PicoGreen dsDNA Assay; Life Technologies) and assessed for purity by NanoDrop (Thermo Scientific) 260/280 and 260/230 ratio measurements. The DNA integrity of the fresh frozen samples was checked with a TapeStation system (Agilent Biotechnologies) using Genomic DNA ScreenTape (Agilent Biotechnologies).
Mangiante L., Alcala N., Sexton-Oates A., Di Genova A., Gonzalez-Perez A., Khandekar A., Bergstrom E.N., Kim J., Liu X., Blazquez-Encinas R., Giacobi C., Le Stang N., Boyault S., Cuenin C., Tabone-Eglinger S., Damiola F., Voegele C., Ardin M., Michallet M.C., Soudade L., Delhomme T.M., Poret A., Brevet M., Copin M.C., Giusiano-Courcambeck S., Damotte D., Girard C., Hofman V., Hofman P., Mouroux J., Cohen C., Lacomme S., Mazieres J., de Montpreville V.T., Perrin C., Planchard G., Rousseau N., Rouquette I., Sagan C., Scherpereel A., Thivolet F., Vignaud J.M., Jean D., Ilg A.G., Olaso R., Meyer V., Boland-Auge A., Deleuze J.F., Altmuller J., Nuernberg P., Ibáñez-Costa A., Castaño J.P., Lantuejoul S., Ghantous A., Maussion C., Courtiol P., Hernandez-Vargas H., Caux C., Girard N., Lopez-Bigas N., Alexandrov L.B., Galateau-Salle F., Foll M, & Fernandez-Cuesta L. (2023). Multiomic analysis of malignant pleural mesothelioma identifies molecular axes and specialized tumor profiles driving intertumor heterogeneity. Nature Genetics, 55(4), 607-618.
+ Open protocol
+ Expand
3

EMS Mutagenesis and Screening for Mutants

Check if the same lab product or an alternative is used in the 5 most similar protocols
EMS mutagenesis [64] (link) was performed on a strain (# 1284, see Text S1) carrying the EGA-GFP (integrated at two chromosomal locations to increase GFP fluorescence). F2 animals derived from ≈10.000 F1s were screened. Candidate mutations were identified as previously described [65] (link). Each mutant was back-crossed four times against the parental strain before genome sequencing. Genomic DNAs (gDNAs) were isolated using Gentra Puregene Tissue Kit 4 g (Qiagen). DNA libraries were created from 50 ng of gDNA (Nextera DNA kit from Illumina). The sequencing data were generated using Hi Seq 2000 (Illumina).
Tocchini C., Keusch J.J., Miller S.B., Finger S., Gut H., Stadler M.B, & Ciosk R. (2014). The TRIM-NHL Protein LIN-41 Controls the Onset of Developmental Plasticity in Caenorhabditis elegans. PLoS Genetics, 10(8), e1004533.
+ Open protocol
+ Expand
4

Mutagenesis Screen for Mutant Identification

Check if the same lab product or an alternative is used in the 5 most similar protocols
The mutagenic chemical, ethyl methanesulfonate (EMS), was applied to the R-1 reporter-carrying strain (rrrSi482), according to described procedures with some modifications [49 (link)]. To facilitate the screen, 10 F1s were pooled on a single plate and F2s from 300 pools were screened. Candidate mutants, expressing the R-1 GFP, were identified with a florescent microscope.
Then, to identify underlying mutations, the isolated mutants were backcrossed to P0. In case of viable mutants, animals descending from ~30 singled homozygous F2s were used for DNA extraction. In case of the sterile mutant, heterozygous mutants were backcrossed to P0. Then, animals descending from ~30 singled heterozygous F2s were used for DNA extraction (S2 Fig). Genomic DNA was extracted using Gentra Puregene Tissue Kit 4g (Qiagen) and submitted for whole genomic DNA sequencing with HiSeq2000.
Guo Y., Tocchini C, & Ciosk R. (2021). CLK-2/TEL2 is a conserved component of the nonsense-mediated mRNA decay pathway. PLoS ONE, 16(1), e0244505.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!