Hexamethyldisilizane

Manufactured by Merck Group

Sourced in United States

Hexamethyldisilizane is a chemical compound used in various industrial applications. It is a clear, colorless liquid with a low viscosity. Hexamethyldisilizane is commonly used as a reagent in organic synthesis and as a surface treatment agent in the production of semiconductor devices.

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Lab products found in correlation

Em ace600 sputter coater, by Leica (2 mentions) Sigma 300 sem, by Zeiss (2 mentions) Thermanox coverslip, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Glutaraldehyde, by Electron Microscopy Sciences (1 mentions) 0.1 m cacodylate buffer, by Merck Group (1 mentions) Sc7620, by Quorum Technologies (1 mentions) Versa 3d, by Thermo Fisher Scientific (1 mentions) Glutaraldehyde, by Agar Scientific (1 mentions) Sodium cacodylate buffer, by Merck Group (1 mentions) Em ace600, by Leica (1 mentions) Glutaraldehyde, by Merck Group (1 mentions) Sputter coater 108 auto, by Cressington (1 mentions) Evo ma10, by Zeiss (1 mentions) Aluminium specimen stubs, by Agar Scientific (1 mentions) Sigma 300 scanning electron microscope, by Zeiss (1 mentions) Paraformaldehyde (pfa), by Thermo Fisher Scientific (1 mentions) Jsm it100, by JEOL (1 mentions)

8 protocols using hexamethyldisilizane

Biofilm Formation on Knitted Implants

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Biofilm formation on knitted implants
after 24 h of incubation
was imaged with scanning electron microscopy (SEM). After incubation,
the samples were fixed in 4% paraformaldehyde and 1% glutaraldehyde
for 4 h at room temperature followed by a washing step with PBS and
dehydration using a graded ethanol series. In order to reduce sample
surface tension, samples were immersed in hexamethyldisilizane (Sigma-Aldrich)
for 30 min and air dried before mounting on aluminum SEM stubs and
sputter-coating with a 6 nm platinum-palladium layer, using a Leica
EM ACE600 sputter coater (Leica Microsystems). Pictures were taken
at random locations with a magnification of 100 × and 3000 ×
using a Zeiss Sigma 300 SEM (Zeiss).

Verhorstert K.W., Guler Z., de Boer L., Riool M., Roovers J.P, & Zaat S.A. (2020). In Vitro Bacterial Adhesion and Biofilm Formation on Fully Absorbable Poly-4-hydroxybutyrate and Nonabsorbable Polypropylene Pelvic Floor Implants. ACS Applied Materials & Interfaces, 12(48), 53646-53653.

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SEM Analysis of Cell Morphology on Thermanox

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Cells were grown on Thermanox coverslips (Nunc; Thermo Fisher Scientific). After a quick wash (<10 s) in fresh culture medium without protein, cells were fixed with 2% paraformaldehyde (Electron Microscopy Science) and 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M cacodylate buffer (Sigma-Aldrich), pH 7.4. Cells were rinsed in 0.1 M cacodylate buffer (3 × 5 min). Dehydration was performed in a graded ethanol series (30, 50, 70, 90, 95, and 100% ethanol, 3 × 2 min each step). After dehydration, specimens were rinsed with hexamethyldisilizane (Sigma-Aldrich) and dried in an oven (60°C for 2 h). Samples were mounted on aluminum stubs by fixing the coverslip with double-sided carbon tape and sputter coated (SC7620; Quorum) with gold/palladium (5–8 nm). Cells were examined with a scanning electron microscope (Versa 3D; FEI) using the ETD detector.

Heusermann W., Hean J., Trojer D., Steib E., von Bueren S., Graff-Meyer A., Genoud C., Martin K., Pizzato N., Voshol J., Morrissey D.V., Andaloussi S.E., Wood M.J, & Meisner-Kober N.C. (2016). Exosomes surf on filopodia to enter cells at endocytic hot spots, traffic within endosomes, and are targeted to the ER. The Journal of Cell Biology, 213(2), 173-184.

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Biofilm Fixation and SEM Imaging

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Following biofilm formation, the culture medium was removed, and samples were rinsed in PBS. They were then fixed by addition of 2.5% glutaraldehyde (electron microscopy (EM) grade, Agar Scientific, UK) in 0.1 M sodium cacodylate buffer (Sigma UK) pH 7.2 for 10 min at room temperature, followed by dehydration in an ethanol series of increasing concentration from 20%–100%. The 100% ethanol was replaced by hexamethyldisilizane (Sigma, UK), which was removed by evaporation. The samples with biofilms were acquired with the same SEM methodology as described earlier.

Barkarmo S., Longhorn D., Leer K., Johansson C.B., Stenport V., Franco‐Tabares S., Kuehne S.A, & Sammons R. (2019). Biofilm formation on polyetheretherketone and titanium surfaces. Clinical and Experimental Dental Research, 5(4), 427-437.

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Scanning Electron Microscopy Sample Preparation

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Samples were fixed in 4% paraformaldehyde and 1% glutaraldehyde for 1 hr at room temperature and dehydrated using an ethanol series. To reduce sample surface tension, samples were immersed in hexamethyldisilizane (Sigma-Aldrich) for 30 min and air dried. Before imaging, samples were mounted on aluminum SEM stubs and sputter-coated with a 4 nm platinum-palladium layer using a Leica EM ACE600 sputter coater (Leica Microsystems, Illinois, USA). Images were acquired at 2 kV using a Zeiss Sigma 300 SEM (Zeiss, Germany).

Arts J.J., Mahlandt E.K., Grönloh M.L., Schimmel L., Noordstra I., Gordon E., van Steen A.C., Tol S., Walzog B., van Rijssel J., Nolte M.A., Postma M., Khuon S., Heddleston J.M., Wait E., Chew T.L., Winter M., Montanez E., Goedhart J, & van Buul J.D. (2021). Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration. eLife, 10, e66074.

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Ultrastructural Analysis of Meniscus Tissue

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Axial and circumferential slices obtained from blocks of meniscus tissue were fixed with a 2% glutaraldehyde (Sigma-Aldrich Co., St. Louis, MO) in PBS solution. Samples were then dehydrated in a graded series of ethanol (20%, 50%, 70%, 90%, 100%) and dried by immersion in hexamethyldisilizane (Sigma-Aldrich Co., St. Louis, MO) [26 ]. The samples were sputter-coated with gold/palladium (Cressington Scientific 108auto Sputter Coater, Redding, CA). High-resolution SEM images were obtained using an Environmental Scanning Electron Microscope (FEI/Phillips XL-30 FEG ESEM, Hillsboro, OR).

Travascio F., Devaux F., Volz M, & Jackson A.R. (2020). MOLECULAR AND MACROMOLECULAR DIFFUSION IN HUMAN MENISCUS: RELATIONSHIPS WITH TISSUE STRUCTURE AND COMPOSITION. Osteoarthritis and cartilage, 28(3), 375-382.

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Visualizing Single-Subspecies Biofilm Architecture via SEM

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In order to examine single-subspecies biofilm architecture using SEM, biofilms were grown on poly-L-lysine coated plastic (Thermanox™) coverslips in 24-well plates. Biofilms were fixed using 2.5% glutaraldehyde (Agar Scientific, Stansted, United Kingdom) in 0.1 M sodium cacodylate buffer (pH 7.4, BioWorld, Dublin, Ireland) for 10 min at room temperature. Following fixation, biofilms were dehydrated with increasing ethanol concentrations (20–100%) and incubated for 10 min at each step.
Finally, drying agent hexamethyldisilizane (Sigma-Aldrich/Merck, Darmstadt, Germany) was applied and left to evaporate overnight. Coverslips with biofilms were mounted onto aluminium specimen stubs (Agar Scientific, Stansted, United Kingdom), sputter coated with two layers of gold and visualised using a scanning electron microscope (Zeiss EVO MA10). A visible layer of biofilm was chosen after visual evaluation of each specimen under low magnification (50X) and recorded at 1000X and 5000X magnification.

Muchova M., Balacco D.L., Grant M.M., Chapple I.L., Kuehne S.A, & Hirschfeld J. (2022). Fusobacterium nucleatum Subspecies Differ in Biofilm Forming Ability in vitro. Frontiers in Oral Health, 3, 853618.

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SEM Imaging of Cell Proliferation and ECM

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Cell proliferation and ECM deposition on the
scaffolds were imaged after 14 and 28 days of incubation using SEM.
The samples were fixed in 4% paraformaldehyde and 1% glutaraldehyde
for 4 h at room temperature, followed by dehydration using a graded
ethanol series. The samples were immersed in hexamethyldisilizane
(Sigma-Aldrich, USA) for 30 min to reduce sample surface tension.
After air-drying, the samples were mounted on aluminum stubs and sputter-coated
with a 6 nm platinum–palladium layer using a Leica EM ACE600
sputter coater (Leica Microsystems, Germany). Micrographs were taken
at random locations at a magnification of 100× using a Zeiss
Sigma 300 scanning electron microscope (Zeiss, Germany).

Verhorstert K., Gudde A., Weitsz C., Bezuidenhout D., Roovers J.P, & Guler Z. (2022). Absorbable Electrospun Poly-4-hydroxybutyrate Scaffolds as a Potential Solution for Pelvic Organ Prolapse Surgery. ACS Applied Bio Materials, 5(11), 5270-5280.

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Caco-2 Cell Growth on Membranes

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To investigate whether our membranes provide a good support for the growth and brush border formation of Caco-2 cells, the membranes cultured with cells were imaged using SEM (JEOL JSM-IT 100, Tokyo, Japan) after 7,14 and 21 days of culture. Membranes with cells were fixed in 4% (v/v) paraformaldehyde (Thermoscientific, Rockford, USA) for 1 h at room temperature, dehydrated in water-ethanol solutions following a gradient (volume ratio water: ethanol of 100:0, 50:50, 25:75, 10:90, 5:95 and 0:100) and dried after dipping into hexamethyldisilizane (Sigma-Aldrich, Saint Louis, USA) overnight. The dried membranes were placed on the SEM holders and sputter-coated with a 2 nm-thick gold layer prior to imaging.

Gommers L.M.M., Skrzypek K., Bolhuis-Versteeg L., Pinckaers N.E.T., Vrijhof R., van der Wijst J., de Baaij J.H.F., Stamatialis D, & Hoenderop J.G.J. (2019). Development of a villi-like micropatterned porous membrane for intestinal magnesium and calcium uptake studies. Acta biomaterialia, 99.

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