The MONO ZetaView® (PMX-120, Particle Metrix, Meerbusch, Germany) was used with 488 nm scatter mode to validate the quantity and size of particles. Using filtered PBS solution (HyClone laboratories, UT, USA), the EV samples were diluted to 107–108 particles/ml. For all samples, the detailed parameters for accurate analysis were tuned with sensitivity 75, shutter 100, minimum trace length 15, and cell temperature 25 °C. Transmission electron microscopy (TEM; Hitachi, H-7600, 80 kV, Japan) was used to examine the morphology of EVs. The EV solution was dried on a Formvar/copper grid with a carbon coating of 150 mesh (FCF150-CU, Electron Microscopy Sciences, USA). EVs were stained with 7% uranyl acetate or gadolinium acetate solution and dried for negative staining on a copper grid. The Formvar/copper grid was put on the grid box for TEM investigation after drying. The pH was detected by a pH meter using a microelectrode (InLab Ultra-Micro-ISM, Mettler Toledo, OH, USA) for small sample volumes.
Inlab ultra micro ism
Manufactured by Mettler Toledo
Sourced in United States
The InLab Ultra-Micro-ISM is a laboratory instrument designed for precise pH and ion measurements. It features a micro-sized electrode for sample volumes as low as 50 microliters. The device provides accurate and reliable measurements for a wide range of applications requiring small sample sizes.
Automatically generated - may contain errors
Lab products found in correlation
Sevencompact s220 micro kit, by Mettler Toledo (2 mentions)
H 7600, by Hitachi (1 mentions)
Mono zetaview, by Particle Metrix (1 mentions)
Fcf150 cu, by Electron Microscopy Sciences (1 mentions)
Sevencompact s210, by Mettler Toledo (1 mentions)
Au480 clinical chemistry analyzer, by Beckman Coulter (1 mentions)
G1780, by Promega (1 mentions)
8 protocols using inlab ultra micro ism
1
Characterization of Extracellular Vesicles by ZetaView and TEM
2
Monitoring pH and Redox Potential
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At the initial and final time points, 1 ml was taken from all biotic and abiotic enrichments to measure pH and oxidation–reduction potential (Inlab Ultra-micro ISM and Redox microelectrodes, Mettler Toledo, Seven Compact, Columbus, Ohio, USA).
3
Precision pH Measurement Technique
4
pH Titration Profiles Determination
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The pH titration profiles of was determined in a glass vessel using a pH meter (SevenCompactS210, Mettler Toledo, Columbus, OH, USA) equipped with a pH glass combined electrode (InLab Ultra-Micro-ISM, Mettler Toledo, Columbus, OH, USA) for pH measurements in aqueous solutions. The pH electrode was calibrated in aqueous solution with three standard buffers of pH 4.01, 7.00 and 9.21. The temperature of the titration vessel was controlled at 20 °C using a thermostatic water bath. For measuring the pH profile 5 mL of 0.01 M solution was freshly prepared in water and titrated with 0.01 M NaOH/HCl under continuous magnetic stirring. At least two repeated measurements were performed for the determination of pH profile.
5
Biochemical analysis of body fluids
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Blood and seminal plasma were analyzed for 12 biochemical parameters, including sodium (Na+), potassium (K+), calcium (Ca2+), magnesium (Mg2+), chlorine (Cl−), phosphate (PO43−), partial pressure of carbon dioxide (pCO2), total protein, cholesterol, glucose, triglyceride, and urea, using an Olympus AU 480 Clinical Chemistry Analyzer (Beckman Coulter, USA). Osmolality was measured by a freezing point depression osmometer (Osmomat 030; Genotec GmbH, Berlin, Germany), and pH was recorded using a micro-pH electrode (inlab ultra-micro-ISM; Mettler‐Toledo, Melbourne, Victoria, Australia).
6
Temperature and pH Monitoring in Embryo Storage
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The temperature of the holding medium was recorded with a data logger (testo 175, Testo, West Chester, PA, USA) every 10 min for 24 h. Briefly, a second tube with the same volume of holding medium as for the embryo was placed inside the Equitainer, and the fine sensor of the data logger was placed inside this holding medium for temperature monitoring. The pH was assessed in a sample of the holding medium immediately before and after embryo storage for 24h. The pH was assessed by a pH meter (SevenCompact S220-micro-kit, Mettler Toledo, Columbus, OH, USA) using the microelectrode (InLab Ultra-Micro-ISM, Mettler Toledo, Columbus, OH, USA) for small sample volumes.
7
Measuring Cell Culture Parameters
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For the testing of the potential of hydrogen (pH), we extract 15 µl culture medium from the slice culture system using a detecting instrument (InLab Ultra Micro-ISM, Mettler Toledo); Glucose was tested using a detecting instrument (GlucCell™, Brookfield), using 3 µl of culture medium; For LDH (lactate dehydrogenase), a detection kit (G1780, Promega Corp.) in a 96-well plate was used. All the processes were conducted using the operation manuals provided by the suppliers.
8
Equitainer Temperature and pH Monitoring
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The temperature of the holding medium was recorded with a data logger (testo 175, Testo, West Chester, PA, USA) every 10 min for 24 h. Briefly, a second tube with the same volume of holding medium as for the embryo was placed inside the Equitainer and the fine sensor of the data logger was placed inside this holding medium for temperature monitoring.
The pH was assessed in a sample of the holding medium immediately before and after embryo storage for 24 h. The pH was assessed by a pH meter (SevenCompact S220-micro-kit, Mettler Toledo, Columbus, OH, USA) using the microelectrode (InLab Ultra-Micro-ISM, Mettler Toledo) for small sample volumes.
The pH was assessed in a sample of the holding medium immediately before and after embryo storage for 24 h. The pH was assessed by a pH meter (SevenCompact S220-micro-kit, Mettler Toledo, Columbus, OH, USA) using the microelectrode (InLab Ultra-Micro-ISM, Mettler Toledo) for small sample volumes.
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