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In situ apoptosis detection kit

Manufactured by Thermo Fisher Scientific
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The In situ Apoptosis Detection kit is a tool used to detect and visualize apoptosis, a form of programmed cell death, in tissue samples. The kit provides a reliable and standardized method for identifying apoptotic cells through the labeling of DNA fragmentation, a hallmark of apoptosis.

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Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection Apoptosis detection kit

2 protocols using in situ apoptosis detection kit

1

TUNEL Staining for Apoptosis

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TUNEL staining was performed via an In situ Apoptosis Detection kit (Invitrogen), as the manufacturer instructed. After TUNEL staining, heart sections were stained with antibodies specific for CMs or hMSCs. Samples were imaged under a confocal microscope, and five views were randomly selected from each section to quantify the number of TUNEL-positive cells.
Park S.J., Kim R.Y., Park B.W., Lee S., Choi S.W., Park J.H., Choi J.J., Kim S.W., Jang J., Cho D.W., Chung H.M., Moon S.H., Ban K, & Park H.J. (2019). Dual stem cell therapy synergistically improves cardiac function and vascular regeneration following myocardial infarction. Nature Communications, 10, 3123.
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2

Apoptosis Detection in Adenomyosis Uterus

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Apoptotic cells in the myometrium of adenomyosis uterus were further detected using a TUNEL colorimetric staining (In situ apoptosis detection kit, Invitrogen) according to the manufacturer's instructions. TUNEL staining was performed with fluorescein‐dUTP for apoptotic cell nuclei, and DAPI was used to stain all cell nuclei. After labelling, the TUNEL‐positive cells were visualized and counted under a fluorescence microscopy.
Che X., Wang J., He J., Yu Q., Sun W., Chen S., Zou G., Li T., Guo X, & Zhang X. (2019). A new trick for an old dog: The application of mifepristone in the treatment of adenomyosis. Journal of Cellular and Molecular Medicine, 24(2), 1724-1737.
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