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Phosphorylated mek1 2 p mek ser221 166f8

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Phosphorylated MEK1/2 (p-MEK) (Ser221) 166F8 is an antibody product that specifically detects MEK1/2 protein phosphorylated at serine 221. This antibody can be used to identify the phosphorylation state of the MEK1/2 protein, which is a key component of the MAPK signaling pathway.

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Lab products found in correlation

Goat anti rat igg hrp sc 2032, by Santa Cruz Biotechnology (2 mentions) Activation state antibodies, by Cell Signaling Technology (2 mentions) Mlk4 nbp1 41081, by Novus Biologicals (2 mentions) Rat anti dykddddk flag 200474 21, by Agilent Technologies (2 mentions) Immun star goat anti mouse igg hrp conjugate 1705047, by Bio-Rad (2 mentions) Anti rabbit igg hrp conjugate w401b, by Promega (2 mentions) β actin c 4 sc 47778, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

MEK1/2 (167 citations) P-MEK (139 citations) P-MEK1/2 (95 citations) P-MEK1 (5 citations) Phosphorylated MEK (4 citations) Phosphorylated MEK1/2 (2 citations)

2 protocols using phosphorylated mek1 2 p mek ser221 166f8

1

Immunoblotting Analysis of Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting was performed with the following Santa Cruz Biotechnology (Dallas, TX, USA) antibodies: MLK1 (N-20) sc-19120, MLK3 (C-20) sc-536, B-Raf (F-3) sc-55522, MEK1/2 (12-B) sc-436, ERK2 (C-14) sc-154, JNK1 (C-17) sc-474, GST (B-14) sc-138, β-actin (C-4) sc-47778, and goat anti-rat IgG-HRP sc-2032. Activation state antibodies from Cell Signaling Technology (Beverly, MA, USA) include: phosphorylated MEK1/2 (p-MEK) (Ser221) 166F8, phosphorylated ERK1/2 (p-ERK) (Thr202/Tyr204) #4370S, and phosphorylated JNK (p-JNK) (Thr183/Tyr185) 9251L. Other antibodies used in this study were MLK4 NBP1-41081 (Novus Biologicals, Littleton, CO, USA), rat anti-DYKDDDDK (FLAG) 200474-21 (Agilent Technologies, Santa Clara, CA, USA), anti-rabbit IgG HRP conjugate W401B (Promega), and Immun-Star goat anti-mouse IgG-HRP conjugate 1705047 (Bio-Rad, Hercules, California, USA).
Schroyer A.L., Stimes N.W., Abi Saab W.F, & Chadee D.N. (2017). MLK3 phosphorylation by ERK1/2 is required for oxidative stress-induced invasion of colorectal cancer cells. Oncogene, 37(8), 1031-1040.
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2

Immunoblotting Analysis of Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting was performed with the following Santa Cruz Biotechnology (Dallas, TX, USA) antibodies: MLK1 (N-20) sc-19120, MLK3 (C-20) sc-536, B-Raf (F-3) sc-55522, MEK1/2 (12-B) sc-436, ERK2 (C-14) sc-154, JNK1 (C-17) sc-474, GST (B-14) sc-138, β-actin (C-4) sc-47778, and goat anti-rat IgG-HRP sc-2032. Activation state antibodies from Cell Signaling Technology (Beverly, MA, USA) include: phosphorylated MEK1/2 (p-MEK) (Ser221) 166F8, phosphorylated ERK1/2 (p-ERK) (Thr202/Tyr204) #4370S, and phosphorylated JNK (p-JNK) (Thr183/Tyr185) 9251L. Other antibodies used in this study were MLK4 NBP1-41081 (Novus Biologicals, Littleton, CO, USA), rat anti-DYKDDDDK (FLAG) 200474-21 (Agilent Technologies, Santa Clara, CA, USA), anti-rabbit IgG HRP conjugate W401B (Promega), and Immun-Star goat anti-mouse IgG-HRP conjugate 1705047 (Bio-Rad, Hercules, California, USA).
Schroyer A.L., Stimes N.W., Abi Saab W.F, & Chadee D.N. (2017). MLK3 phosphorylation by ERK1/2 is required for oxidative stress-induced invasion of colorectal cancer cells. Oncogene, 37(8), 1031-1040.
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