Qiaprep amp viral rna um kit

Manufactured by Qiagen

Sourced in United States

The QIAprep Viral RNA UM Kit is a laboratory equipment product designed for the purification of viral RNA from various sample types. It is used to extract viral RNA for further analysis and downstream applications.

Automatically generated - may contain errors

Lab products found in correlation

Sars cov 2 n1 n2 assay kit, by Qiagen (2 mentions) Qiaamp viral rna mini kit, by Qiagen (1 mentions)

Close spelling variants of this product

QIAprep kit QIAprep

3 protocols using qiaprep amp viral rna um kit

SARS-CoV-2 Infection Detection via RT-qPCR

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Quantitative real-time PCR (RT qPCR) was performed as described previously (20 (link)) at the time of sample collection for flow cytometry experiments. This procedure was essential to ensure that no SARS-CoV-2 infection or reinfection occurred in the HC and recovered volunteers. The RNA isolated from nasopharyngeal or oropharyngeal swabs was extracted (QIAprep& Viral RNA UM Kit, QIAGEN, USA) and amplified by one-step RT qPCR (SARS-CoV-2 N1+N2 Assay Kits, QIAGEN, USA).

de Sousa Palmeira P.H., Peixoto R.F., Csordas B.G., de Medeiros I.A., de Azevedo F.D., Veras R.C., Janebro D.I., Amaral I.P, & Keesen T.S. (2023). Differential regulatory T cell signature after recovery from mild COVID-19. Frontiers in Immunology, 14, 1078922.

+ Open protocol

+ Expand

SARS-CoV-2 Genome Sequencing Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The selected samples were subjected to extraction and quantitative detection using RNA extraction and real-time PCR-based virus detection system QIAprep & amp Viral RNA UM Kit (Qiagen). Ct value of selected samples was (< 20) as demanded by the sequencing laboratory. The RNA was then extracted from the viral transport medium by QIAamp Viral RNA Mini Kit (Qiagen), and transported to the Intergen commercial company (Ankara, Turkey) on dry ice for whole-genome sequencing.

Ahmed J.Q., Maulud S.Q., Al-Qadi R., Mohamed T.A., Tayib G.A., Hassan A.M., Taha L.S., Qasim K.M, & Tawfeeq M.A. (2022). Sequencing and mutations analysis of the first recorded SARS-CoV-2 Omicron variant during the fourth wave of pandemic in Iraq. The Brazilian Journal of Infectious Diseases, 26(5), 102677.

+ Open protocol

+ Expand

SARS-CoV-2 Detection by RT-qPCR

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

During blood collection for flow cytometry experiments, quantitative real-time PCR (RT qPCR) RT qPCR was performed as described previously [18] (link). RNA was isolated from nasopharyngeal or oropharyngeal swabs, and RNA was extracted (QIAprep& Viral RNA UM Kit QIAGEN, USA) and amplified by one-step RT qPCR (SARS-CoV-2N1 + N2 Assay Kits - QIAGEN, USA). This procedure is essential to ensure no SARS-CoV-2 infection or reinfection in the HC and recovered patients, respectively.

Csordas B.G., de Sousa Palmeira P.H., Peixoto R.F., Comberlang F.C., de Medeiros I.A., Azevedo F.L., Veras R.C., Janebro D.I., Amaral I.P., Barbosa-Filho J.M, & Keesen T.S. (2022). Is IFN expression by NK cells a hallmark of severe COVID-19?. Cytokine, 157, 155971.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!