Cells were grown and selected for using standard molecular biology techniques (Sherman, 2002 (link)). Yeast transformations were performed using lithium acetate–based methods (Gietz et al., 1995 (link)). In brief, log phase cultures were transformed for 45 min at 42°C with the vector/PCR product in a mixture of ∼40% wt/vol PEG-3350 (Sigma-Aldrich), 0.1 M lithium acetate (Sigma-Aldrich), and 0.28 mg/ml SS carrier DNA (Sigma-Aldrich). Transformants were always selected for on appropriate synthetic media. Table S1 shows the strains used in this study.
Ss carrier dna
Manufactured by Merck Group
SS carrier DNA is a laboratory reagent used in molecular biology and genetics research. It is a single-stranded DNA molecule that serves as a carrier for other DNA samples during various experimental procedures, such as DNA purification or transformation. The primary function of SS carrier DNA is to enhance the efficiency of these processes by providing a substrate for the binding and transport of the target DNA molecules.
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Lab products found in correlation
2 protocols using ss carrier dna
1
Standard Yeast Transformation Protocol
2
Yeast Transformation and Dilution Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were grown and selected for using standard molecular biology techniques. Yeast transformations were performed using lithium acetate–based methods (Gietz et al., 1995 (link)). In brief, log phase 5-ml cultures were transformed for 45 min at 42°C with the plasmid/PCR product in a mixture containing ~40% wt/vol PEG-3350 (Sigma-Aldrich), 0.1 M lithium acetate (Sigma-Aldrich), and 0.28 mg/ml SS carrier DNA (Sigma-Aldrich). Dilution assays were spotted on appropriate media by 1:10 serial dilutions and incubated at the indicated temperature.
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