Dulbecco s modified eagle s medium dmem

Manufactured by Sartorius

Sourced in Israel, United States, China

Dulbecco's modified Eagle's medium (DMEM) is a cell culture medium widely used in the laboratory for the growth and maintenance of various cell types. It provides essential nutrients, vitamins, and salts required for cell proliferation and survival. DMEM is a well-established and commonly used medium in the field of cell biology and biotechnology.

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Lab products found in correlation

Fetal bovine serum (fbs), by Sartorius (28 mentions) L glutamine, by Sartorius (11 mentions) Streptomycin, by Sartorius (6 mentions) Penicillin streptomycin, by Sartorius (6 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions) Penicillin, by Sartorius (5 mentions) Rpmi 1640 medium, by Sartorius (4 mentions) Fetal calf serum (fcs), by Sartorius (4 mentions) Bovine serum albumin (bsa), by Merck Group (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Pen strep, by Sartorius (3 mentions) Sodium pyruvate, by Sartorius (3 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Sk mel 5, by American Type Culture Collection (2 mentions) Sk mel 28, by American Type Culture Collection (2 mentions) Rpmi medium, by Sartorius (2 mentions) Ldh cytotoxicity assay kit, by Beyotime (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions)

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DMEM medium (58 citations)

55 protocols using dulbecco s modified eagle s medium dmem

Cell Culture Conditions for Cancer Cell Lines

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HepG2, FOCUS, Hep3B, Mahlavu, Huh7, Hep40,
and PLC-PRF-5 hepatocellular carcinoma cells; MCF-7, MDA-MB-231, MDA-MB-468,
SKBR3, and ZR-75 breast cancer cells; and normal-like epithelial MCF-10A
breast cells were cultured in low-glucose Dulbecco’s modified
Eagle’s medium (DMEM) (Biological Industries-BI) supplemented
with 10% fetal bovine serum (FBS) (Gibco/Thermo Fisher Scientific),
2 mM l-Glutamine (Gibco/Thermo Fisher Scientific), 100 Units/mL
Penicillin/Streptomycin, and 0.1 mM non-essential
amino acid (Gibco/Thermo Fisher Scientific). SNU475 hepatocellular
carcinoma cells were maintained in RPMI (Biological Industries-BI)
supplemented with 10% fetal bovine serum (FBS) (Gibco/Thermo Fisher
Scientific), l-Glutamine (Gibco/Thermo Fisher Scientific),
and 100 Units/mL Penicillin/Streptomycin, while ZR-75 breast cancer
cells were cultured RPMI (Biological Industries-BI) supplemented with
10% fetal bovine serum (FBS) (Gibco/Thermo Fisher Scientific), 1×
sodium pyruvate, and %4.5 glucose. Normal-like MCF-10A breast cells
were sustained in DMEM/HAM’S F12 (Hyclone) supplemented with
10% fetal bovine serum (FBS) (Gibco/Thermo Fisher Scientific), EGF
(20 ng/mL), Hydrocortisone (0.5 mg/mL), cholera toxin (100 ng/mL),
insulin (10 μg/mL), and 100 Units/mL Penicillin/Streptomycin.
The cells were cultivated at 37 °C in a humidified incubator
under 5% CO₂.

Turanlı S., Nalbat E., Lengerli D., İbiş K., Güntekin Ergün S., Akhan Güzelcan E., Muyan M., Cetin-Atalay R., Çalışkan B, & Banoglu E. (2022). Vicinal Diaryl-Substituted Isoxazole and Pyrazole Derivatives with In Vitro Growth Inhibitory and In Vivo Antitumor Activity. ACS Omega, 7(41), 36206-36226.

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Visualizing Microtubule Dynamics in NIH3T3 Cells

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NIH3T3 cells were grown in Dulbecco's modified Eagle's medium (DMEM) (Biological Industries, Israel) supplemented with 12% fetal bovine serum (FBS) (Biological Industries, USA). Regions of interest from tmf/ara160 were amplified by PCR reaction as described before [3 (link)] using the appropriate primers described in S1 Table. These amplified DNA sequences were cloned into pIRES-EGFP plasmids. Plasmids containing the tmf/ara160 sequences fused to gfp were transiently transfected into NIH3T3 cells using the LipofectAMINE 2000 reagent, according to the manufacturer's instructions (Invitrogen, USA), and as previously described [30 (link)]. The transfected cells were treated or untreated with 10μM Colchicine (Sigma-Aldrich, Israel) to allow microtubules disturbance [18 (link)] and were taken for immunocytochemistry analysis, as described above. The presented images represent one out of four different transfection and immuno-staining experiments which yielded similar results.

Elkis Y., Bel S., Rahimi R., Lerer-Goldstein T., Levin-Zaidman S., Babushkin T., Shpungin S, & Nir U. (2015). TMF/ARA160 Governs the Dynamic Spatial Orientation of the Golgi Apparatus during Sperm Development. PLoS ONE, 10(12), e0145277.

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Lung Cancer Cell Line Cultivation and Inhibitor Treatment

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The human LUAD cell lines A549 and H1299 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and authenticated by short tandem repeat (STR) profiling. The HEK293T cell line was acquired from the Integrated Hospital of Traditional Chinese Medicine (TCM-Integrated Hospital) of Southern Medical University (Guangzhou, P.R. China). These cell lines were analyzed for mycoplasma and were verified to be mycoplasma-free. A549 and H1299 cells were cultured in RPMI 1640 medium (Biological Industries, Bet Haemek, Israel) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO, USA). HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Biological Industries) supplemented with 10% FBS. All cells were incubated in a humidified incubator at 37°C with 5% CO₂. The AKT1 inhibitor MK-2206 2HCl and the MAPK3/1 inhibitor SCH772984 were purchased from Selleck Chemicals (Houston, TX, USA). For inhibitor treatment, 5 mmol/L MK-2206 2HCl and/or 5 mmol/L SCH772984, or dimethyl sulfoxide (DMSO; Sigma-Aldrich) alone for control, were freshly added to the cell culture every 24 h.

He T., Shen H., Wang S., Wang Y., He Z., Zhu L., Du X., Wang D., Li J., Zhong S., Huang W, & Yang H. (2020). MicroRNA-3613-5p Promotes Lung Adenocarcinoma Cell Proliferation through a RELA and AKT/MAPK Positive Feedback Loop. Molecular Therapy. Nucleic Acids, 22, 572-583.

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NSCLC Cell Lines Culture Protocol

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The NSCLC lines, PC-9 and PC9/GR with EGFR exon 19 deletion [delE746-A750], without T790M mutations and MET gene amplification (kind gifts from Dr. Zhou Caicun, Shanghai pulmonary hospital, Shanghai, China), HCC827 (a gift from Peking Union Medical College), H1975 with EGFR L858R/T790M mutation (a kind gift from 3D Medicines, Shanghai, China), and H1650 with EGFR exon 19 deletion and PTEN loss (a kind gift from 3D Medicines, Shanghai, China) [46 (link)] were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Biological Industries, Kibbutz Beit-Haemek, Israel) or RPMI 1640 (Biological Industries) supplemented with 10% fetal bovine serum (FBS) (Biological Industries), 100 μg/mL streptomycin and 100 U/mL penicillin (KeyGEN BioTECH, Nanjing, China) in a humidified cell incubator at 37 °C with an atmosphere of 5% CO₂.

Jia Z., Wang K., Duan Y., Hu K., Zhang Y., Wang M., Xiao K., Liu S., Pan Z, & Ding X. (2022). Claudin1 decrease induced by 1,25-dihydroxy-vitamin D3 potentiates gefitinib resistance therapy through inhibiting AKT activation-mediated cancer stem-like properties in NSCLC cells. Cell Death Discovery, 8, 122.

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Molecular Mechanisms of Lipid Metabolism

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DMSO, α-linolenic acid, and BSA were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Biological Industries (Kibbutz Beit-Haemek, Israel). Antibodies against FASN (C2065), GAPDH (#5174), Phosoho-Akt (Ser473#4060), and Akt (#4691) were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Antibodies against Bax (ab32503), Bcl-2 (ab32124), IRE1 (ab124945), PERK (ab229912), and ATF6 (ab227830) were purchased from Abcam (Boston, MA, USA).

Fan H., Huang W., Guo Y., Ma X, & Yang J. (2022). α-Linolenic Acid Suppresses Proliferation and Invasion in Osteosarcoma Cells via Inhibiting Fatty Acid Synthase. Molecules, 27(9), 2741.

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Lung Cancer Cell Culture Protocol

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Human cell lines SW900 and H520 (squamous lung carcinoma), A549 (lung adenocarcinoma), DMS53 (small cell lung carcinoma) and HFL-1 (lung fibroblasts), were obtained from the American Type Culture Collection (ATCC). SQCLC, adenocarcinoma and lung fibroblasts cells were cultured (passage number 10–25) in Roswell Park Memorial Institute medium (RPMI, Cat# 01-104), Dulbecco’s modified Eagle’s medium (DMEM, Cat#01-055) and Ham’s F-12 (Cat#01-095) (Biological Industries), respectively. All of them were supplemented with 10% heat-inactivated fetal bovine serum (FBS Gibco™; Cat#10270106, Life Technologies), 100 units/mL penicillin, 100 μg/mL streptomycin, and 2 mM L-glutamine (all from Biological Industries). Non-essential amino acids (NEAA; Cat#X0557, Biowest; 1:100) were also used for HFL-1 culture (Biological Industries). 15 mM of HEPES buffer solution (Cat#03-025, Biological Industries) was also used for H520 culture. Cells were grown at 37 °C in a humidified incubator (Thermo Fisher Scientific Inc.) with 5% CO₂ atmosphere. The cells were mycoplasma tested using a standard PCR technique after thawing.

Martínez-García D., Pérez-Hernández M., Korrodi-Gregório L., Quesada R., Ramos R., Baixeras N., Pérez-Tomás R, & Soto-Cerrato V. (2019). The Natural-Based Antitumor Compound T21 Decreases Survivin Levels through Potent STAT3 Inhibition in Lung Cancer Models. Biomolecules, 9(8), 361.

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Lysosomal Dysfunction and Cell Death

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Coffee beans were bought from Yunli Coffee Company (Yunnan, China). Z-VAD-FMK (S7023), RIP (D94C12) XP (3493S) and RIP3 (D8J3L) (15828S) have been reported in previous literature [20 (link)]. The Lysosensor Green DND-189 probe was purchased from Thermo Fisher Scientific Co. Ltd. (Waltham, MA, USA). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were obtained from Biological Industries (BioInd; Kibutz Beit Haemek, Israel). Anti-GAPDH antibody (ZB002) and propidium iodide (PI)-Annexin V/fluorescein isothiocyanate (FITC) apoptosis detection kit (556547) were obtained from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China). Goat anti-mouse IgG (1036-05) and goat anti-rabbit (4050-05) were obtained from Southern Biotechnology Associates, Inc. (Birmingham, AL, USA). DQ^TM red bovine serum albumin (BSA) (D12051) was purchased from Thermo Fisher Scientific Co., Ltd. (Waltham, MA, USA).

Chu L., Zhang Y., He L., Shen Q., Tan M, & Wu Y. (2023). Carbon Quantum Dots from Roasted Coffee Beans: Their Degree and Mechanism of Cytotoxicity and Their Rapid Removal Using a Pulsed Electric Field. Foods, 12(12), 2353.

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Cell Culture Conditions for Cancer Cell Lines

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Human cervical cancer HeLa cell, mouse fibroblast L929 cell, human thyroid cancer BHT101 cell, and human breast cancer Mcf-7 cells were obtained from the Shanghai Cell Bank (Shanghai, China). Human liver carcinoma HepG2, Sk-Hep-1, and Bel-7402 cells were obtained from Xiguang Chen laboratory at the Ocean University of China (Qingdao, China), Medical College of Qingdao University (Qingdao, China), and Baoqin Han laboratory at the Ocean University of China (Qingdao, China), respectively. The HepG-2 cells, HeLa cells, BHT-101 cells, Mcf-7 cells, Sk-Hep-1 cells, and Bel-7402 cells were cultured in Dulbecco‘s modified eagle’s medium (DMEM) (Biological Industries, Kibbutz Beit, Israel), supplemented with 15% fetal bovine serum (FBS) (Biological Industries, Israel) and 1% penicillin-streptomycin (Biological Industries, Israel). The L929 cells were cultured in DMEM supplemented with 10% FBS (Gibco, Invitrogen, Carlsbad, CA, USA) and 1% penicillin-streptomycin. All cell lines were cultured at 37 °C with 5% CO₂ incubator (ThermoFisher, Waltham, MA, USA).

Li J., Han S., Zhu Y, & Dong B. (2023). Halorotetin A: A Novel Terpenoid Compound Isolated from Ascidian Halocynthia rotetzi Exhibits the Inhibition Activity on Tumor Cell Proliferation. Marine Drugs, 21(1), 51.

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Tomato and Rosemary Extract Bioactivity

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Tomato extract (Lycomato^TM) and rosemary extract were a gift of Lycored Ltd., Beer Sheva, Israel). The tomato extract, prepared by ethyl acetate extraction of tomato pulp, contained 6% lycopene, other tomato carotenoids (phytoene and phytofluene above 1%, beta-carotene above 0.2%), and additional fat-soluble tomato components such as natural tocopherols (above 1.5%) and phytosterols (1.1–2.5%). The remainder were triacylglycerols (70–72%), monoacylglycerols (8–9%), and phospholipids (7–8%). The rosemary extract was prepared by extraction with 80% ethanol. Its composition was only partially determined to contain carnosic acid (20.2%) and carnosol (2.5%) as the main polyphenols. 17β-estradiol was purchased from Sigma-Aldrich (Rehovot, Israel). Carotenoids were dissolved in tetrahydrofuran (THF), solubilized in cell culture medium, and their final concentration was measured as described previously [28 (link),29 ,30 (link)]. Rosemary extract and estradiol were dissolved in ethanol. H₂O₂ 30% and THF, containing 0.025% butylated hydroxytoluene as an antioxidant, were purchased from Sigma-Aldrich. Dulbecco’s modified Eagle’s medium (DMEM), dextran-coated charcoal-treated fetal bovine serum (DCC-FBS), Hanks’ solution, and 1M HEPES buffer were purchased from Biological Industries (Beth Haemek, Israel).

Darawsha A., Trachtenberg A., Levy J, & Sharoni Y. (2021). The Protective Effect of Carotenoids, Polyphenols, and Estradiol on Dermal Fibroblasts under Oxidative Stress. Antioxidants, 10(12), 2023.

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Comprehensive Cysteine Labeling Protocol

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Iodoacetamide (IAA), glutathione (GSH), DL-dithiothreitol (DTT), tris(2-chloroethyl) phosphate (TCEP), copper sulfate (CuSO₄), S-methyl methanethiosulfonate (MMTS) and calcium chloride (CaCl₂) were purchased from Sigma-Aldrich unless otherwise specified. Fmoc-Cys and N-ethylmaleimide (NEM) was obtained from Heowns and J&K Scientific, respectively. Biotin-PEG3-azide, DADPS-Biotin-Azide and click chemistry auxiliary reagents (TBTA and BTTAA) was purchased from Click chemistry tools. Cy3-Azide and desthioBiotin-PEG3-azide was obtained from Okeanos and Confluore Biotechnology, respectively. Four peptides containing one cysteine, GCSWDYKN was synthesized from GL Biochem and the others were purchased from SciLight Biotechnology. All cell culture related reagents, such as fetal bovine serum, Dulbecco’s modified Eagle’s medium (DMEM) and penicillin-streptomycin were purchased from Biological Industries (BI). Three kinds of proteins containing one free cysteine residues, β-lactoglobulin A, bovine serum albumins and papain were obtained from Sigma-Aldrich. All antibodies used for immunoblotting were purchased from Abcam except HRP-labeled Streptavidin (Beyotime Biotechnology), and other chemical or biological reagents were obtained from commercial suppliers without any manipulation.

Jiang Z., Tang Y., Lu J., Xu C., Niu Y., Zhang G., Yang Y., Cheng X., Tong L., Chen Z, & Tang B. (2023). Identification of sulfhydryl-containing proteins and further evaluation of the selenium-tagged redox homeostasis-regulating proteins. Redox Biology, 69, 102969.

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