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Mouse anti dic

Manufactured by Merck Group

Mouse anti-DIC is a primary antibody that recognizes the Dynein Intermediate Chain (DIC), a component of the dynein motor complex. It can be used for the detection and localization of DIC in various applications, such as immunoblotting and immunocytochemistry.

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2 protocols using mouse anti dic

1

Antibody Procurement for Protein Analysis

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The mouse anti-APP, mouse anti-KIF3B and rabbit anti-ULK1 were purchased from Cell Signaling. Rabbit anti-Atg16L1 and rabbit anti-Atg5 were purchased from ABclonal (China). Rabbit anti-β-actin, rabbit anti-LC3B-Specific, rabbit anti-P50, rabbit anti-DHC1, rabbit anti-DlC3 and rabbit anti-P62/SQSTM1 were purchased from Proteintech (US). Mouse anti-DIC was bought from Millipore. Rabbit anti-RILP and rabbit anti-Lamp2 were purchased from Abcam. Mouse anti-Huntingtin and mouse anti-BICD2 were purchased from Santa Cruz. All the secondary antibodies were purchased from Bioworld Technology.
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2

AAV-based Protein Trafficking Assay

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pAAV-SYN-MCS-EGFP-3FLAG-miR30shRNA(Dync1i2), pAAV-SYN-Dync1i2-2A-EGFP-3FLAG, pAOV-SYN-MCS-EGFP-3FLAG and pAAV-SYN-MCS-EGFP-3FLAG were from Obio Technology (Shanghai, China). The plasmids si-m-Dync1i2_001(CGAAGAGAATGATAGCAAA), si-m-Dync1i2_002(GGTGCTAAGCTGTCATTAA), si-m-Dync1i2_003(GGACAACTAAGAATAACAA), si-m-NC were constructed by Ribo Biotechnology Company(Guangzhou, China). Rabbit anti-β-actin, rabbit anti-LC3B-Specific, rabbit anti- P50, rabbit anti-KIF5B and rabbit anti-P62/SQSTM1 were purchased from Proteintech (US). Mouse anti-P150 was tought from BD. Mouse anti-DIC was bought from Millipore. Mouse anti-Rab7 and rabbit anti-Lamp2 were purchased from Abcam. Mouse anti-4G8(β-Amyloid, 17-24) was bought from Biolegend. All the secondary antibodies were purchased from Bioworld Technology.
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