Emem media

Manufactured by American Type Culture Collection

Sourced in United States

EMEM (Eagle's Minimum Essential Medium) is a cell culture media formulation developed by Harry Eagle. It provides a balanced salt solution and essential nutrients required for the maintenance of various cell lines in vitro. The core function of EMEM is to support the growth and proliferation of cells in a controlled laboratory environment.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

EMEM culture media (2 citations) EMEM growth media (2 citations) EMEM cell culture media (2 citations)

27 protocols using emem media

Investigating TGFβ-VHL signaling in renal cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

In this study, we used the ACHN and A498 human renal cell carcinoma cell lines, to investigate the biological function of TGFβ-signaling in correlation to VHL. All cell lines were authenticated by STR profiling (IdentiCell, Denmark). ACHN and A498 cell lines were purchased from ATCC (Wesel, Germany). ACHN cells were cultured in EMEM media (ATCC) supplemented with 10% FBS. A498 cells were cultured in RPMI media (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% FBS.
These cell lines were selected based on their responsiveness to TGF-β [13,24], their expression of VHL: ACHN (VHL^+/+) [47] and A498 (VHL ^−/-) [47,48], and that they should express HIF-1α and HIF-2α [49]. It has previously been reported that ACHN is of papillary type [50–52]. However, early studies have classified it as poorly differentiated clear cell type [53], and gene expression analysis have revealed similarities to clear cell tumors, especially when concerning the MYC pathway [54]. The A498 cell line is known to harbor a VHL mutation, which is causing the loss of expression of VHL [47,48]. Thus, A498 cell has frequently been used as a model for ccRCC [48,55,56] even if one study has described it as papillary type [57].

Mallikarjuna P., Raviprakash T.S., Aripaka K., Ljungberg B, & Landström M. (2019). Interactions between TGF-β type I receptor and hypoxia-inducible factor-α mediates a synergistic crosstalk leading to poor prognosis for patients with clear cell renal cell carcinoma. Cell Cycle, 18(17), 2141-2156.

+ Open protocol

+ Expand

Establishment of Human Medulloblastoma Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human MB cell lines Daoy, D-283 and D-341and were obtained from American Type Culture Collection (ATCC, Manassas, VA). HD-MB03 MB cell line was obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Germany). The cell lines were authenticated by their respective supplier. These cell lines were cultured in EMEM media (ATCC) containing 10% FBS, 1% penicillin, and 1% streptomycin (Invitrogen, CA). The cultures were maintained in a humidified incubator at 5% CO2 and 95% air atmosphere at 37°C. All cultures were passaged at 80-90% confluence.

Chaturvedi N.K., Kling M.J., Coulter D.W., McGuire T.R., Ray S., Kesherwani V., Joshi S.S, & Sharp J.G. (2018). Improved therapy for medulloblastoma: targeting hedgehog and PI3K-mTOR signaling pathways in combination with chemotherapy. Oncotarget, 9(24), 16619-16633.

+ Open protocol

+ Expand

Cell Culture Protocols for HMC3 and hAD-MSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

HMC3 cells were purchased from ATCC (ATCC^® CRL-3304™) and grown in EMEM Media (ATCC, VA, USA), supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin/streptomycin (Gibco-Life Technologies, USA) in multilayer flasks (Nest Scientific, USA) at 37 °C and 5% CO₂ in a forced air incubator. hAD-MSCs were kindly donated by Dr. Ricordi’s Lab (Diabetes Research Institute, University of Miami, Miami, FL, USA); details of their isolation and characterization have been previously described^{68 (link),69}. These cells were grown in Prime-XV expansion serum-free Media (91135, Fujifilm Irvine Scientific, CA, USA) and 1% penicillin/streptomycin (Gibco-Life Technologies, USA) at 37 °C and 5% CO₂ in a forced air incubator. For all experiments, hAD-MSCs were used up to passage number 5.

Garcia-Contreras M, & Thakor A.S. (2021). Human adipose tissue-derived mesenchymal stem cells and their extracellular vesicles modulate lipopolysaccharide activated human microglia. Cell Death Discovery, 7, 98.

+ Open protocol

+ Expand

Stable Fluorescent Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

MDA-MB-231 and HT-1080 cancer cells (ATCC) were grown to 85% confluence in 25 cm² tissue culture flasks in complete growth media (EMEM media [ATCC], 10% fetal bovine serum, and 1% penicillin/streptomycin) at 37°C in a 5% CO₂ incubator. Media from each flask was replaced with 5 ml of high glucose DMEM media supplemented with 5 mM sodium butyrate, 0.1 mM PEI, and 40 µl of the triple reporter lentivirus. After 12 hours, the viral media was replaced with complete growth media, and the cell lines were expanded in culture for 3 weeks. A FACS Vantage SE Diva was used to collect the top 1.5% brightest cells from each cell line based on E2-Crimson fluorescence. The MDA-MB-231 and HT-1080 FACS populations were expanded in culture for 3 weeks before use in all subsequent experiments.

Levin R.A., Felsen C.N., Yang J., Lin J.Y., Whitney M.A., Nguyen Q.T, & Tsien R.Y. (2014). An Optimized Triple Modality Reporter for Quantitative In Vivo Tumor Imaging and Therapy Evaluation. PLoS ONE, 9(5), e97415.

+ Open protocol

+ Expand

Culture Methods for Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Culture methods and target cells are summarized in Table 1. BEAS-2B (No. CRL-9609; ATCC, Manassas, VA) immortalized human lung epithelial cells were cultured in BEGM media with a Single Quot supplement kit (No. CC-3170; Lonza, Basel, Switzerland) at 37°C in 5% CO₂. LNCaP (No. CRL-1740; ATCC) human prostate adenocarcinoma cells were cultured using phenol-red free RPMI-1640 (No. 11835; Life Technologies, Grand Island, NY) with 10% fetal bovine serum (FBS) and penicillin/streptomycin at 37°C in 5% CO₂. PLHC-1 (No. CRL-2406; ATCC) fish (Poeciliopsis lucida) hepatoma cells were cultured using EMEM media (No. 30–2003; ATCC) with 5% FBS and penicillin/streptomycin at 30°C in 5% CO₂.

Kabadi P.K., Vantangoli M.M., Rodd A.L., Leary E., Madnick S.J., Morgan J.R., Kane A, & Boekelheide K. (2015). Into the depths: Techniques for in vitro three-dimensional microtissue visualization. BioTechniques, 59(5), 279-286.

+ Open protocol

+ Expand

Sunitinib Dissolution and Breast Cancer Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sunitinib (Selleckchem, Houston, TX) was dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO) and diluted in cell culture media or PBS for in vitro use. Human breast cancer cell lines MDA-MB-231, MDA-MB-468, SKBR-3, MCF-7, and HCC-1419 were obtained from American Type Culture Collection (ATCC, Manassas, VA). The MDA-MB-231, HCC-1419, and MDA-MB-468 cell lines were cultured in RPMI media (Sigma-Aldrich). MCF-7 cell lines were cultured in EMEM media (ATCC). SKBR-3 cells were cultured in McCoy's 5A media (Gibo; Rockville, MD). In all media, 10% fetal bovine serum (Atlantic Biologicals, Miami, FL), 1% Pen/strep (Gibco), 1% sodium pyruvate, 1% NEAA, and 1% glutamine (Gibco) were added and cultured cells were maintained at 37°C and 5% CO₂ humidified atmosphere.

Ghimirey N., Steele C., Czerniecki B.J., Koski G.K, & Showalter L.E. (2021). Sunitinib Combined with Th1 Cytokines Potentiates Apoptosis in Human Breast Cancer Cells and Suppresses Tumor Growth in a Murine Model of HER-2pos Breast Cancer. International Journal of Breast Cancer, 2021, 8818393.

+ Open protocol

+ Expand

Primary Rodent Fibroblast Culture Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary rodent fibroblasts were from our established collection (Seluanov et al., 2008). Cells were cultured as described (Seluanov et al., 2010). Briefly, fibroblasts were cultured at 37 °C, 5% CO₂, and 3% O₂ incubator, except the NMR fibroblasts that were cultured at 32 °C. All cells were cultured on polystyrene‐treated plastic dishes (Corning) with EMEM media (ATCC) supplied with 15% FBS (Gibco), 100 units/mL penstrep.

Ke Z., Mallik P., Johnson A.B., Luna F., Nevo E., Zhang Z.D., Gladyshev V.N., Seluanov A, & Gorbunova V. (2017). Translation fidelity coevolves with longevity. Aging Cell, 16(5), 988-993.

+ Open protocol

+ Expand

Cell Culture Protocol for HEK293, SH-SY5Y, and Daoy Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293 cells and SH-SY5Y cells were cultured in 4.5 g/L glucose DMEM
media (Gibco #11965) supplemented with 10% FBS and 1%
pen/strep at 37°C and 5% CO₂. Daoy cells were
cultured in 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium
bicarbonate EMEM media (ATCC #30–2003) supplemented with
10% FBS and 1% pen/strep at 37°C and 5%
CO₂.

Jiang Y., Gaudin A., Zhang J., Agarwal T., Song E., Kauffman A.C., Tietjen G.T., Wang Y., Jiang Z., Cheng C.J, & Saltzman W.M. (2018). A "top-down" approach to actuate poly(amine-co-ester) terpolymers for potent and safe mRNA delivery. Biomaterials, 176, 122-130.

+ Open protocol

+ Expand

Cultivation of HUH7 and Vero Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell lines were maintained in a humidified 5% CO2 environment at 37 °C. HUH7 and HUH7.5 cells (Human hepatocyte derived cells from carcinoma, Apath LLC, new York, NY, USA) were cultured in DMEM (4500 mg/L “high glucose,” + L-glutamine, + 25 mM HEPES, - Sodium Pyruvate; Life Technologies 12430-062) supplemented with fetal bovine serum (FBS, Gibco; 10% FBS was used for cytotoxicity assays and 2% was used in antiviral plaque assays), penicillin (100 I.U./mL), and streptomycin (100 μg/mL, ATCC) and 1% non-essential amino acids (NEAA; Sigma Aldrich M7145-100ML) at a density of 2×10⁵ – 2×10⁶ cells/mL. Vero cells (ATCC, CCL-81) were cultured in EMEM media (ATCC) supplemented with FBS (10% FBS was used for cytotoxicity assays and 2% was used in antiviral plaque assays), penicillin (100 I.U./mL), and streptomycin (100 μg/mL) at a density of 2×10⁵ – 2×10⁶ cells/mL. HUH7 Cells were verified by short tandem repeat (STR) profiling by Creative Bioarray.

Passow K.T., Caldwell H.S., Ngo K.A., Arnold J.J., Antczak N.M., Narayanan A., Jose J., Sturla S.J., Cameron C.E., Ciota A.T, & Harki D.A. (2021). A Chemical Strategy for Intracellular Arming of an Endogenous Broad-Spectrum Antiviral Nucleotide. Journal of medicinal chemistry, 64(20), 15429-15439.

+ Open protocol

+ Expand

Malignant Melanoma Cell Lines and Growth Conditions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human malignant melanoma cell lines (part of NCI-60 panel of human cancer cells) - SK-MEL-5, SK-MEL-28, MALME-3M, MDA-MB-435S and normal human skin fibroblast cells MALME-3 cells were obtained from American Type Culture Collection (ATCC; Manassas, VA). SK-MEL-5 and SK-MEL-28 were grown and maintained in EMEM media (ATCC), while MALME-3M and MDA-MB-435S were grown in IMDM (ATCC) and RPMI-1640 (ATCC) respectively, as indicated by ATCC protocols. Complete growth media was supplemented with 10% fetal bovine serum (FBS; ATCC) and 1X antibiotic-antimycotic (Thermo Fisher Scientific, Waltham, MA). MALME-3 cells were grown in McCoy's medium (ATCC) supplemented with 15% FBS and 1X antibiotic-antimycotic. Cells were grown at 37C / 5% CO₂ in a humidified incubator. Half the media was replaced every 48 hr. No hGH was present in the media or added externally unless specifically mentioned. For hGH treatment, 16 hr. after seeding (or 24 hr. post-transfection), the cells were serum-starved for 2 hr. in serum free growth media and hGH (PBS as control) was added at the mentioned concentrations (5, 50, 150 ng/mL). Cells were subsequently incubated for 24 hr. before RNA extraction. Recombinant hGH was purchased from Antibodies Online (Atlanta, GA).

Basu R., Wu S, & Kopchick J.J. (2017). Targeting growth hormone receptor in human melanoma cells attenuates tumor progression and epithelial mesenchymal transition via suppression of multiple oncogenic pathways. Oncotarget, 8(13), 21579-21598.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!