T p70s6k

Manufactured by Cell Signaling Technology

Sourced in United States

T-p70S6K is a laboratory product that detects the phosphorylation of the ribosomal protein S6 kinase, p70S6K, a key downstream target of the mTOR signaling pathway. This product can be used to monitor cellular signaling events related to cell growth, proliferation, and metabolism.

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P70S6K

4 protocols using t p70s6k

Galangin-Modulated Apoptotic Signaling

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Galangin was purchased from Shanghai Yuanye Bio-Technology (Shanghai, China). The primary antibodies against Bcl-2, p-Akt, t-Akt, p-p70S6K, t-p70S6K, Bax, p21, p53, DR5, caspase-3, -7, -8 and -9 were purchased from Cell Signaling Technology, Inc. (Dancers, MA, USA). The primary antibodies against Gapdh, cmyc and p53 were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA).

Huang H., Chen A.Y., Ye X., Guan R., Rankin G.O, & Chen Y.C. (2020). Galangin, a Flavonoid from Lesser Galangal, Induced Apoptosis via p53-Dependent Pathway in Ovarian Cancer Cells. Molecules, 25(7), 1579.

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Apoptosis Pathway Regulation Assay

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WST-1 assay kit was purchased from Daeillab (Daeillab, Korea). and dihydrotestosterone (DHT) were purchased from Sigma Aldrich (Sigma Aldrich, USA). Specific antibodies such as Bcl-2, Bax, APAF-1 was purchased from Santa Cruz Biotechnology (Santa Cruz Biotechnology, USA). Specific antibodies such as t-mTOR, p-mTOR, LC3, p62 Cyclin E1, p-cdk2, t-p70 S6K, p-p70 S6K1, t-4E-BP1, p-4E-BP1 were obtained from Cell Signaling Technology (Beverly, USA). and Specific antibodies such as β-actin and Active-caspase-3 antibodies were purchased from Abcam (Cambridge, USA). Muse Cell Cycle Kit (MCH100106) and Muse Cell Analyzer (PB4455ENEU) were purchased from Millipore (EMD Millipore Corporation, Germany).

Nam G.H., Jo K.J., Park Y.S., Kawk H.W., Yoo J.G., Jang J.D., Kang S.M., Kim S.Y, & Kim Y.M. (2019). The peptide AC 2 isolated from Bacillus-treated Trapa japonica fruit extract rescues DHT (dihydrotestosterone)-treated human dermal papilla cells and mediates mTORC1 signaling for autophagy and apoptosis suppression. Scientific Reports, 9, 16903.

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Adenylyl Cyclase Protein Expression Analysis

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Expression of adenylyl cyclase related proteins were analyzed using
Agilent Gene Expression Microarray technique by PhalanxBio Inc. (CA, San Diego).
C1C2 protein was detected by anti-AU1 antibody (Fitzgerald, 1:2,000) or
anti-AC5/6 antibody (Santa Cruz, 1:200). Additional antibodies included: GAPDH
(Fitzgerald, 1:20,000); cMLCK (Abgent, 1:1,000); MLC2v (Synaptic Systems,
1:1,000) PKA catalytic subunit (BD Transduction, 1:1,000); p-ERK1/2, p38,
p-PKARII α and β (Santa Cruz Biotech, 1:200); PLB (Affinity
Bioreagents, 1:5,000); phospho-16-PLB (Badrilla, 1:3,000); S100A1 (Epiyomics,
1:1,000); SERCA2a antibody (Enzo, 1:1,000); phospho-αB-crystallin
(CryAB) and total CryAB antibodies (Enzo, 1:1,000); P-308-Akt, P-473-Akt, T-Akt,
p-GSK3a/b, p-MDM2, P-p70S6K, T-p70S6K, and phospho-S22/23 troponin I (TnI) (Cell
Signaling, 1:1,000 each); Vinculin (Sigma, 1:100,1000).

Gao M.H., Lai N.C., Giamouridis D., Kim Y.C., Tan Z., Guo T., Dillmann W.H., Suarez J, & Hammond H.K. (2016). Cardiac-Directed Expression of Adenylyl Cyclase Catalytic Domain Reverses Cardiac Dysfunction Caused by Sustained Beta-Adrenergic Receptor Stimulation. JACC: Basic to Translational Science, 1(7), 617-629.

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Protein Expression Analysis in Pancreatic Cancer Cell Lines

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CFPAC-1 and AsPC-1 cells were harvested, washed, and lysed with RIPA buffer (Beyotime) containing protease inhibitor cocktail (Roche) for 30 min on ice. The total protein was extracted and quantified using a BCA kit (Beyotime) according to the manufacturer’s instructions. The protein samples were separated by electrophoresis using a 4%~20% gel and then transferred onto PVDF membranes (Millipore). The blotted membranes were blocked and incubated with primary antibodies overnight at 4°C. After 3 washes with TBST and a 1-h incubation with an HRP-conjugated secondary antibody (Proteintech) at room temperature, the membranes were developed using an enhanced chemiluminescence detection system (Bio-Rad Laboratories) to detect the protein. The primary antibodies against PC4 were obtained from Sigma, while the primary antibodies against β-actin, p-mTOR, t-mTOR, p-p70s6k, t-p70s6k, p-4EBP1, t-4EBP1, CDK4, CDK6, Cyclin D and Cyclin E were obtained from Cell Signaling Technology.

Su X., Yang Y., Ma L., Luo P., Shen K., Dai H., Jiang Y., Shuai L., Liu Z., You J., Min K., Shi C, & Chen Z. (2020). Human Positive Coactivator 4 Affects the Progression and Prognosis of Pancreatic Ductal Adenocarcinoma via the mTOR/P70s6k Signaling Pathway. OncoTargets and therapy, 13, 12213-12223.

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