Wiesner reagent (phloroglucinol/HCl) staining of plant tissue for 5 min was used to visualize lignification [5 (link)]. Two grams of phloroglucinol were dissolved in 100 mL of 95% alcohol and then filtered into 40 mL of concentrated hydrochloric acid. A razor blade was used to dissect the leaf tissue prior to observation. Lignified structures appeared pink or fuchsia in color. Auto-fluorescence within the stem sections was observed with the aid of an EVOS smart fluorescence microscope (Thermo Fisher, Waltham, MA, USA).
Evos smart fluorescence microscope
Manufactured by Thermo Fisher Scientific
Sourced in United States
The EVOS smart fluorescence microscope is a compact, digital imaging system designed for fluorescence microscopy. It features LED illumination and high-resolution image capture capabilities. The core function of the EVOS smart fluorescence microscope is to enable visualization and analysis of fluorescently-labeled samples.
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Lab products found in correlation
2 protocols using evos smart fluorescence microscope
1
Visualization of Plant Lignification
2
Lignification Analysis of Leaf Veins and Stems
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The transverse section of the leaf veins and stem were used by Weisner staining and microscopy. Weisner reagent (phloroglucinol/HCl) was used to stain the plant tissue for 5 min before it was visualized for lignification under a microscope [48 (link)]. The lignified structures appeared pink or fuchsia in the bright field images. Auto-fluorescence within the leaf and stem sections was also observed with the aid of an EVOS smart fluorescence microscope (Thermo Fisher, Waltham, MA, America).
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