Mrc 5 cells

H2291 cells were obtained from the American Type Culture Collection and MRC-5 cells were obtained from Korean Cell Line Bank. PC9 cells were provided by J.C. Lee (Korea Institute of Radiological and Medical Science, Seoul, Korea). H2291, PC9, and all PDCs were cultured in R10 medium. MRC-5 cells were cultured in MEM supplemented with 10% fetal bovine serum, 5% penicillin/streptomycin, and 1% primocin. All cells were replenished with medium every three days and maintained in a 5% CO2 incubator at 37 °C. Cells were passaged at ratios of 1:2 to 1:6 using TrypLE (Invitrogen).

Human embryonic kidney cell HEK293, human non-small-cell lung cancer NCI-H1993, and NCI-H1975 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). Human normal lung fibroblast MRC-5 cells and human non-small-cell lung cancer A549 cells were purchased from the Korean Cell Line Bank (Seoul, Korea). Human non-small-cell lung cancer PC-9 cells were provided by Drs. Jin Kyung Rho and Jae Cheol Lee (Asan Medical Center, Seoul, Korea). The A549, PC9, NCI-H1993, and NCI-H1975 cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (10% FBS, Gibco, Grand Island, NY, USA) and antibiotics-antimycotics (100 units/mL penicillin G sodium, 100 μg/mL streptomycin, and 250 ng/mL amphotericin B). MRC-5 and HEK293 cells were cultured in DMEM medium supplemented with 10% FBS and antibiotics-antimycotics. Cells were incubated at 37 °C with 5% CO₂ in a humidified atmosphere.

Human lung carcinoma H292 cells were obtained from the American Type Culture Collection (Manassas, VA, USA). Embryonic lung fibroblast (MRC-5) cells were provided from the Korean Cell Line Bank (Seoul, Korea). H292 cells were cultured in RPMI 1640 medium, and MRC-5 cells were cultured in MEM medium supplemented with 10% FBS and antibiotics-antimycotics (PSF; 100 units/mL penicillin G sodium, 100 μg/mL streptomycin, and 250 ng/mL amphotericin B). Cells were incubated at 37 °C with 5% CO₂ in a humidified atmosphere. Gefitinib-resistant H292 cells (H292-Gef) were developed from the parental H292 cells through continuous exposure to gradually increasing concentrations of Gefitinib (Selleckchem, Houston, TX, USA) and were maintained in RPMI 1640 medium containing 1 μM Gefitinib.