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Fruiting bodies of the artificially cultivated Lentinus edodes were collected from the Taian Academy of Agricultural Sciences (Taian, China), identified by Professor Zhang Jianjun of Shandong Agricultural University, and air-dried and ground into a fine powder with a mill before extraction. The diagnostic kits used for investigating the activities of superoxide dismutase (SOD, A001-3-2), catalase (CAT, A007-2-1), and malondialdehyde (MDA, A003-1-2) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The kits for the tumor necrosis factor-α (TNF-α, MB-2868A), inter-leukin-1β (IL-1β, MB-2776A), and interleukin-6 (IL-6, MB-2899A) investigations were supplied by Jiangsu Meibiao Biological Technology Company Limited (Jiangsu, China). Standard monosaccharides including rhamnose (Rha), ribose (Rib), arabinose (Ara), xylose (Xyl), glucose (Glc), fucose (Fuc), mannose (Man), galactose (Gal), glucuronic acid (Glca), and galacturonic acid (Gala) were from Sigma Chemical Company (St. Louis, USA). All the other reagents and chemicals used in the present work were analytical grade provided by local chemical suppliers in China.

Zearalenone (ZEA, Z2125) and lipopolysaccharide (LPS, SMB00610) were obtained from Sigma (St Louis, Missouri, USA). Quercetin (B20527, purity ≥ 98%) was purchased from Shanghai Yuanye (Shanghai, China). DMEM/F12 (SH30069.03), fetal bovine serum (FBS, SH30406.05), and penicillin-streptomycin (SV30010) were provided by HyClone (Logan, UT, USA). ROS assay kit (S0033S), BCA protein assay kit (P0012), and Annexin V-FITC Apoptosis Detection Kit (C1062S) were purchased from Beyotime Biotechnology (Shanghai, China). Assay kits for superoxide dismutase (SOD, A001-3-2), lactate dehydrogenase (LDH, A020-2-2), total antioxidant capacity (T-AOC, A015-2-1), glutathione (GSH, A006-2-1), and malondialdehyde (MDA, A003-4-1) were purchased from Nanjing Jiancheng (Nanjing, China). The primary antibody against Nrf2 (1:1000, ab137550) as purchased from Abcam (Cambridge, MA, USA). The primary antibodies against HO-1 (1:1000, 10701-1-AP), NQO1 (1:1000, 11451-1-AP), ZO-1 (1:5000, 21773-1-AP), Occludin (1:5000, 27260-1-AP), Claudin 1 (1:1000, 28674-1-AP), β-actin (1:2000, 20536-1-AP), and HRP-conjugated Affinipure Goat AntiRabbit IgG (H+L) (1:4000, SA00001-2) were purchased from Proteintech (Wuhan, China). TRIzol (Cat No. 15596026) and PVDF membranes (Cat No. 24585) were purchased from Thermo Fisher Scientific (Waltham, Massachusetts, USA).

Hepatic total cholesterol (TC, #A111-1-1), triglycerides (TG, #A110-1-1), superoxide dismutase (SOD, #A001-3-2), malondialdehyde (MDA, #A003-1-2), and hydroxyproline (HYP, #A030-2-1) were measured according to the instruction, and all kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Serum TC, TG, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine transaminase (ALT), and aspartate aminotransferase (AST) were measured using automated clinical chemistry analyzer (7020, Hitachi, Japan) in the Center for Drug Safety Evaluation and Research, Shanghai University of Traditional Chinese Medicine (Shanghai, China).

Myocardial tissues (125 mm³) from MIRI rat or normal rats were collected from each experimental group and centrifuged after the addition of 1 mL PBS at 10,000g and 4 °C for 10 min, after which the supernatant was harvested. The protein concentration was measured using a bicinchoninic acid kit (P0011, Beyotime, Shanghai, China). The contents of MDA, SOD and GSH in the myocardial tissues were determined using MDA (A003-1-2), SOD (A001-3-2), and GSH (A006-2-1) assay kits (Nanjing JianCheng Bioengineering Institute, Nanjing, China), respectively.