For three-dimensional printing of the cement pastes, a 3D printer (3D Discovery, regenHU, Villaz-St-Pierre, Switzerland) was used. A print head based on a “time-pressure” principle (DD-135N, 900 002 772, regen HU, Villaz-St-Pierre, Switzerland) in combination with 5 mL and 10 mL cartridges (Nordson EFD, Erkrath, Germany) was chosen to conduct the experiments. For dispensing, compressed air up to 5 bar was utilized to adjust the desired material flow. Conical dispensing tips (cone TT, Nordson EFD, Erkrath, Germany) with different diameters (18, 20, 22, and 27 gauge) were used for extrusion-based printing. Printing was performed at room temperature; no heating or cooling was involved.
3d discovery
Manufactured by RegenHU
Sourced in Switzerland
The 3D Discovery is a bioprinting system developed by RegenHU. It is a precision instrument designed for the fabrication of complex three-dimensional structures using bioinks, hydrogels, and other materials. The system offers high-resolution printing capabilities and is suitable for a range of applications in the fields of tissue engineering, regenerative medicine, and biofabrication.
Automatically generated - may contain errors
Lab products found in correlation
Ha nanoparticles, by Merck Group (2 mentions)
Biocad software, by RegenHU (2 mentions)
Osteoink, by RegenHU (2 mentions)
β tcp microparticles, by Merck Group (1 mentions)
Stemi 508, by Zeiss (1 mentions)
Capa 6500, by Perstorp (1 mentions)
Biocad, by RegenHU (1 mentions)
Geomagic control x, by 3D Systems (1 mentions)
Glass slide, by Carl Roth (1 mentions)
21 protocols using 3d discovery
1
3D Printing of Cement Pastes
2
Colloidal Ink 3D Printing on Glass
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The colloidal inks were loaded into syringes and centrifuged at 2000 rpm for 1 min to remove bubbles prior to printing. Syringes loaded with the inks were mounted in a commercial extrusion-based 3D printer (3D Discovery, RegenHU Ltd., Switzerland). During printing, the ink was pneumatically extruded through a micronozzle onto hydrophobized glass substrates under an applied pressure of 2–3 bars. Nozzle diameters of 410 µm or 580 µm were used to achieve reasonable resolution, while preventing clogging during extrusion (Supplementary Note 7 ). After printing, the materials were dried under a cover at ambient temperature. Samples were also heat treated at 200 °C for 1 h for complete removal of water. During this heating step, the temperature was increased slowly using a ramping period of 3 h to minimize cracking. Note that all the printed structures shown in this work include carbon black.
3
3D Printed Composite Scaffolds for Bone Regeneration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Composite scaffolds were 3D printed, as reported [25 (link)]. Briefly, PCL pellets (Perstorp Caprolactones, Cheshire, UK) were heated up to 90 °C for 20 min followed by the addition of CNTs, and were mixed for more 30 min to ensure homogenous dispersion, before adding HA nanoparticles (Sigma-Aldrich, St. Louis, MO, USA) and/or β-TCP microparticles (Sigma-Aldrich, St. Louis, MO, USA) in order to produce homogeneous mixtures. The specific compositions are shown in Table 1 . The composite scaffolds were fabricated using a screw-assisted extrusion-based 3D printer (3D Discovery, REGENHU, Villaz-Saint-Pierre, Switzerland), considering the protocol used before [25 (link)]. Scaffolds were designed with a 0/90° lay-down pattern, 330 μm filament diameter, and 350 μm pore size [25 (link)]. The dimensions of the final produced scaffolds were 30 mm × 30 mm × 2.5 mm, but to fit into the calvaria of the animals, these scaffolds were further cut within the dimensions of 5 mm × 5 mm × 2.5 mm. Finally, the scaffolds were sterilized in 70% ethanol for 4 h, and then dried overnight.
4
Macroporous Scaffolds for Cell-Laden Microspheres
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The design of macroporous cylindrical scaffold for loading with cell-laden microspheres was performed on BioCAD software (BioCAD 1.1, RegenHU Ltd. Switzerland). The diameter and the height of the scaffolds were 12 mm and 5 mm, respectively. For the initial printed layer, a line space of 200 μm between struts, lower than the average diameter of the microspheres, was printed to prevent microsphere leak out from the interspace. All the other layers had a line space of 800 μm. The layer-by-layer deposition was performed in a crisscross fashion forming an open columnar porosity from bottom to top. A 3D printer (3D Discovery, RegenHU Ltd.) equipped with a screw-based extruder was used for printing the PCL (Sigma–Aldrich, USA, Mn 45′000 g/mol) at a temperature of 75 °C and 70 °C for the printing head and polymer reservoir, respectively. The inner diameter of the printing head was 0.33 mm with a length of 11.2 mm, the screw rotation speed was set at 15 rpm and the printing speed was set up to 6 mm/s.
5
Bioink Printing with Cell-Friendly System
6
3D Printed PCL-Based Scaffolds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PCL, PCL/HA (10 and 20 wt % of HA) and PCL/TCP (10 and 20 wt % of TCP) scaffolds were fabricated using the 3D Discovery (regenHU, Villaz-Saint-Pierre, Switzerland), which is a screw-assisted additive manufacturing system. Scaffolds were fabricated by applying a 0/90° lay-down pattern (see Figure 12 ) and using a melting temperature of 90 °C, a feed rate of 20 mm/s and a screw rotational velocity of 22 rpm.
7
3D Bioprinting of Alginate-Based Inks
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The printability of alginate-based inks was evaluated using a 3D Discovery™ bioprinter (RegenHU, Villaz-St-Pierre, Switzerland) within a biosafety cabinet equipped with a pneumatic extrusion printhead and 25 gauge blunt-end straight needle. Alg1 or Alg1CNC1 pre-gel formulations, each containing red food colouring to aid visualisation, were transferred into 3 mL printing cartridges with a piston. BioCAD™ software was used to design single-layered horizontal lines which were then coded (i.e., G-code) and printed directly inside a 6-well tissue culture plate containing a suspension of agarose fluid gel. Applied air pressure to extrude the ink was varied between 0.018 MPa and 0.050 MPa for printing feed rates of 10, 35, 50, and 60 mm s−1. Images were taken from above the print using a Dino-Lite USB digital microscope and were imported into ImageJ software (National Institute of Health, Bethesda, MA, USA; version 1.52e) to measure filament diameters from six different areas. The resultant values are reported as their mean ± standard deviation (SD).
8
Fabrication and Characterization of Xerogel/Alginate Scaffolds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For extrusion, Xerogel/Alg pastes were filled in 10 mL cartridges with conical needles with 410–840 µm in inner diameter (Globaco GmbH, Rödermark, Germany). Extrusion was performed via compressed air (70–540 kPa air pressure). Scaffolds (6 × 6 mm2) with alternating layer pattern (0°/90°, ABAB) were fabricated with a 3DDiscovery (RegenHU, Villaz-St-Pierre, Swiss), working with 0.5–4 mm/s print head speed. Plotting parameters are summarized in Table 1 . After plotting, scaffolds were immersed in CaCl2 solution (1 M) for 5 min, to allow crosslinking, and dried at 37 °C (Xerogel/Alg scaffold). To estimate the dependency of the thickness of a plotted strand and the nozzle size, photographs of as-printed scaffolds, as well as of SEM images of dried scaffolds, were analyzed, using ImageJ (Vers. 1.53e, W. Rasband, National Institutes of Health, Bethesda, MD, USA). Strand diameter was measured at least 6 times each in horizontal and vertical orientation for calculating mean and standard deviation as a function of printing parameters.
9
GelMA Scaffold 3D Printing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A 3D Discovery™ printing machine (RegenHU Ltd., Villaz-St-Pierre, Switzerland) was used to print the GelMA-based inks. The additive manufacturing process was performed using a direct dispensing print head.
Using BioCAD, square scaffolds were drawn. Scaffolds were printed at room temperature. In order to establish the suitable printing parameters, different printing speeds, ranging from 3 mm/s to 11 mm/s, and pressures in the range of 150–300 kPa, were explored. The scaffolds were UV cured at 360 nm.
Using BioCAD, square scaffolds were drawn. Scaffolds were printed at room temperature. In order to establish the suitable printing parameters, different printing speeds, ranging from 3 mm/s to 11 mm/s, and pressures in the range of 150–300 kPa, were explored. The scaffolds were UV cured at 360 nm.
10
Additive Fabrication of Bone Bricks
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bone bricks with different material compositions (PCL, PCL containing 20 wt% of HA, PCL containing 20 wt% of TCP, and PCL containing 20 wt% of bioglass), were fabricated using the screw-assisted additive manufacturing 3D Discovery (RegenHU, Villaz-St-Pierre, Switzerland) and a continuous path algorithm based on 38 zig-zag double filaments and 14 spiral filaments (Figure 1 ). Similar material compositions were considered for the fabrication of rectangular scaffolds with uniform pore sizes. A total of 3 different pore sizes were considered: 200 μm, 300 μm and 500 μm (Figure 1 ). The overall dimensions of the bone bricks were 31 mm × 26.7 mm × 10 mm. Both bone bricks and rectangular scaffolds were printed using the following processing parameters: 90 °C melting temperature, 20 mm/s deposition velocity and 12 rpm screw rotational velocity. The filaments were extruded using a 0.33 mm diameter needle.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!