Mouse monoclonal anti flag m2 hrp conjugate

Standard immunoblot protocols were used with the following primary antibodies at specified dilutions: mouse monoclonal anti-Flag M2-HRP conjugate (Sigma; A8592; 1:20,000), mouse monoclonal anti-β-actin antibody (Sigma; A5441; 1:10,000), and mouse monoclonal anti-AGO2 antibody (11A9) (43 (link)), a kind gift from Dr. Gunter Meister, Center for Integrated Protein Science Munich (CIPSM), Germany. Secondary horseradish peroxidase-conjugated goat antibodies against rabbit IgG (Santa Cruz Biotechnology; 1:10,000) and mouse IgG (Santa Cruz Biotechnology; 1:10,000) were used to recognize the primary antibodies and allow for detection of the protein-of-interest using chemiluminescence detection technology (Pierce ECL Western Blotting Substrate; Thermo Scientific). The data were visualized using a gel-documentation system (ChemiDoc MP System; BioRad).

Immunoblotting was performed using standard procedures with the following antibodies: mouse monoclonal anti-FLAG M2-HRP conjugate (Sigma; A8592; 1:10,000), mouse monoclonal anti-c-Myc (9E10) HRP conjugate (Santa Cruz; sc-40 HRP; 1:10,000), mouse monoclonal anti-β-actin antibody (Sigma; A5441; 1:10,000), and rabbit polyclonal TPP1 antibody (Bethyl Laboratories; A303-069A; 1:2,500). Secondary horseradish peroxidase-conjugated goat antibodies against rabbit or mouse IgG (Santa Cruz Biotechnology; 1:10,000) were used for detection with chemiluminescence by ECL plus reagents (Pierce ECL Western Blotting Substrate; Thermo Scientific). The data were visualized and quantified using a gel-documentation system (ChemiDoc MP System; BioRad).