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Facsaria fusion sorp flow cytometer

Manufactured by BD
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The BD FACSAria FUSION SORP is a flow cytometer designed for high-performance cell sorting and analysis. It features advanced optics, fluidics, and electronics to deliver accurate and reliable data. The core function of the instrument is to detect, identify, and sort individual cells or particles within a sample based on their physical and fluorescent properties.

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Lab products found in correlation

Multitest 6 colour tbnk reagent, by BD (1 mentions) Trucount tube, by BD (1 mentions)

Close spelling variants of this product

FACSAria SORP flow cytometer BD FACSAria Fusion flow cytometer FACSAria Fusion SORP FACSAria flow cytometer BD Fusion Flow Cytometer FACSAria SORP FACSAria Fusion FACSAria cytometer FACSAria

3 protocols using facsaria fusion sorp flow cytometer

1

Prostate Cell Isolation and Characterization

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Viable human prostate cells were isolated by fluorescence activated cell sorting (FACS) for bulk and single cell sequencing in the UT Southwestern CRI Flow Cytometry Core on a BD FACSAria FUSION SORP flow cytometer and analyzed with FlowJo software as previously published (Henry et al., 2017 (link)). Improved antibody panels based on single cell data were built using titration and fluorescence minus one experiments. The Key Resources Table displays information on antibodies used for flow cytometry.
Henry G.H., Malewska A., Joseph D.B., Malladi V.S., Lee J., Torrealba J., Mauck R.J., Gahan J.C., Raj G.V., Roehrborn C.G., Hon G.C., MacConmara M.P., Reese J.C., Hutchinson R.C., Vezina C.M, & Strand D.W. (2018). A Cellular Anatomy of the Normal Adult Human Prostate and Prostatic Urethra. Cell reports, 25(12), 3530-3542.e5.
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2

Prostate Cell Isolation and Characterization

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Viable human prostate cells were isolated by fluorescence activated cell sorting (FACS) for bulk and single cell sequencing in the UT Southwestern CRI Flow Cytometry Core on a BD FACSAria FUSION SORP flow cytometer and analyzed with FlowJo software as previously published (Henry et al., 2017 (link)). Improved antibody panels based on single cell data were built using titration and fluorescence minus one experiments. The Key Resources Table displays information on antibodies used for flow cytometry.
Henry G.H., Malewska A., Joseph D.B., Malladi V.S., Lee J., Torrealba J., Mauck R.J., Gahan J.C., Raj G.V., Roehrborn C.G., Hon G.C., MacConmara M.P., Reese J.C., Hutchinson R.C., Vezina C.M, & Strand D.W. (2018). A Cellular Anatomy of the Normal Adult Human Prostate and Prostatic Urethra. Cell reports, 25(12), 3530-3542.e5.
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3

Multiparametric Immune Cell Enumeration

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BD multitest 6 colour TBNK reagent and BD Trucount TM tubes were used to enumerate absolute count of T cells, CD4 T cells, CD8 T cells, B cells, and Natural killer (NK) cells using 50 μl of fresh EDTA stabilised blood following stain/lyse/no-wash protocol. Data acquisition was performed on BD FACS Aria Fusion (SORP) Flow cytometer (BD Biosciences) where 5000 events gated on beads count were acquired. Data analysis was carried out using FlowJo software (BD biosciences)
Singh A.K., Kasarpalkar N., Bhowmick S., Paradkar G., Talreja M., Shah K., Tiwari A., Palav H., Kaginkar S., Kulkarni R., Patil A., Kalsurkar V., Agrawal S., Shastri J., Dere R., Bharmal R., Mahale S.D., Bhor V.M., & Patel V. (2021). IL-15 and sMAdCAM: Novel roles in COVID-19 pathogenesis.
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